Sample Processing for Analysis of Hematopoietic Cell Populations in Total Bone Marrow
3:19
Sample Processing for the Analysis of Stromal Cell Populations in Total Bone Marrow
5:13
Sample Processing for the Analysis of Hematopoietic Cell Populations in Crushed and Flushed Bone Marrow
6:28
Results: Phenotypic Analysis of Murine Bone Marrow Hematopoietic Stem and Progenitor Cells and Stromal Niche Cells by Flow Cytometry
7:40
Conclusion
Transcription
The described protocol allows for the phenotypic analysis of bone marrow hematopoietic stem and progenitor cells and bone marrow stromal cells. It can be used to study perturbations to these populations in different settings. Compared with other p
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Here, we describe a simple protocol for the isolation and staining of murine bone marrow cells to phenotype hemopoietic stem and progenitor cells along with the supporting niche endothelial and mesenchymal stem cells. A method to enrich cells located in endosteal and central bone marrow areas is also included.