Methods Article

Detached Maize Sheaths for Live-Cell Imaging of Infection by Fungal Foliar Maize Pathogens

1.3K Views

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06:12 min

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September 15th, 2023

DOI :

10.3791/65755-v

September 15th, 2023

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Renata Belisário¹, Maria F. Torres¹^,², Ester A. S. Buiate¹^,³, Katia V. Xavier¹^,⁴, Etta M. Nuckles¹, Lisa J. Vaillancourt¹

¹Department of Plant Pathology, University of Kentucky, ²Department of Biological Sciences, University of Cincinnati, ³Bayer Crop Science, ⁴Everglades Research and Education Center, University of Florida

Transcript

Summary

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Maize Leaf Sheaths

Fungal Pathogenicity

Plant pathogen Interactions

Real time Imaging

Hemibiotrophic Fungi

Necrotrophic Fungi

Protein Secretion Regulation

Microscopy Protocol

Fungal Colonization

Fluorescent Proteins

Developmental Cytology

Plasmolysis

Nucleic Acids Characterization

Secondary Metabolites

Fungal Protein Effectors

Chapters in this video

0:00

Introduction

0:57

Inoculation of Maize Leaf Sheaths with Pathogenic Fungi for Assessing Fungal Pathogenicity

4:05

Preparation of Fungal Infected Detached Maize Leaf Sheaths for Live‐Cell Imaging

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