JoVE Logo
Faculty Resource Center

Sign In

Direct Agroinoculation of Maize Seedlings by Injection with Recombinant Foxtail Mosaic Virus and Sugarcane Mosaic Virus Infectious Clones

DOI :

10.3791/62277-v

5:56 min

February 27th, 2021

February 27th, 2021

3,872 Views

1Department of Plant Pathology and Microbiology, Iowa State University

An Agrobacterium-based injection (agroinjection) protocol is presented for the inoculation of foxtail mosaic virus and sugarcane mosaic virusclones into maize seedlings. Inoculation in this manner leads to viral infection, virus-induced gene silencing of marker genes, and viral overexpression of GFP.

Tags

Direct Agroinoculation

-- Views

Related Videos

article

Chemically-blocked Antibody Microarray for Multiplexed High-throughput Profiling of Specific Protein Glycosylation in Complex Samples

article

The MultiBac Protein Complex Production Platform at the EMBL

article

Direct Imaging of ER Calcium with Targeted-Esterase Induced Dye Loading (TED)

article

Efficient Agroinfiltration of Plants for High-level Transient Expression of Recombinant Proteins

article

The Tomato/GFP-FLP/FRT Method for Live Imaging of Mosaic Adult Drosophila Photoreceptor Cells

article

Efficient Generation Human Induced Pluripotent Stem Cells from Human Somatic Cells with Sendai-virus

article

Author Spotlight: A Cost-Effective Genomic Workflow for Advancing Rabies Control in Resource-Limited Settings

article

Author Spotlight: Investigating Fungal Pathogenicity Mechanisms in Maize

article

Author Spotlight: Advancements and Challenges in Hepatitis B Virus Detection

article

Sample Preparation, RNA Extraction, PCR Clean Up, and Quantification for Whole Genome Sequencing of Rabies Virus (RABV)

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved