JoVE Logo
Faculty Resource Center

Sign In

Direct Agroinoculation of Maize Seedlings by Injection with Recombinant Foxtail Mosaic Virus and Sugarcane Mosaic Virus Infectious Clones

DOI :

10.3791/62277-v

5:56 min

February 27th, 2021

February 27th, 2021

3,750 Views

1Department of Plant Pathology and Microbiology, Iowa State University

An Agrobacterium-based injection (agroinjection) protocol is presented for the inoculation of foxtail mosaic virus and sugarcane mosaic virusclones into maize seedlings. Inoculation in this manner leads to viral infection, virus-induced gene silencing of marker genes, and viral overexpression of GFP.

Tags

Direct Agroinoculation

-- Views

Related Videos

article

Isolation and Culture of Primary Synovial Macrophages and Fibroblasts from Murine Arthritis Tissue

article

Improved Lipofuscin Models and Quantification of Outer Segment Phagocytosis Capacity in Highly Polarized Human Retinal Pigment Epithelial Cultures

article

Direct Synthesis of EM-Visible Gold Nanoparticles in Cells for Protein Localization Analysis with Well-Preserved Ultrastructure

article

Direct Infusion Device for Molecule Delivery in Plants

article

Generation and Manipulation of Rat Intestinal Organoids

article

Author Spotlight: Streamlining Composite Plant Production via Agrobacterium rhizogenes-Mediated Hairy Root Transformation

article

Author Spotlight: Decoding Corneal Neovascularization with Alkali Burn Model for Future Therapeutic Strategies

article

Author Spotlight: The Production of Recombinant Proteins

article

Optimizing the Rearing Procedure of Germ-Free Wasps

article

Author Spotlight: Investigating the Tolerance of Cabbage Butterflies to Urban Pollutants

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved