Il laboratorio Vondriska è supportato anche da finanziamenti del National Institute Heart, Lung e il Sangue del NIH e la Fondazione Laubisch alla UCLA. EM è destinatario del S. Jennifer Buchwald Graduate Fellowship in Fisiologia presso la UCLA, HC è il destinatario di un American Heart Association Pre-dottorato, MP è il destinatario di un Ruth NIH Kirschstein post-dottorato, e SF è il destinatario di un NIH K99 Award.

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K., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Post-translational+regulation+of+calsarcin-1+during+pressure+overload-induced+cardiac+hypertrophy.">Post-translational regulation of calsarcin-1 during pressure overload-induced cardiac hypertrophy.</a> Journal of molecular and cellular cardiology. 48, 1206-1214 (2010).</li><li>Franklin, S., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Quantitative+analysis+of+the+chromatin+proteome+in+disease+reveals+remodeling+principles+and+identifies+high+mobility+group+protein+b2+as+a+regulator+of+hypertrophic+growth.">Quantitative analysis of the chromatin proteome in disease reveals remodeling principles and identifies high mobility group protein b2 as a regulator of hypertrophic growth.</a> Mol Cell Proteomics. 11, (2012).</li><li>Gramolini, A. O., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Comparative+proteomics+profiling+of+a+phospholamban+mutant+mouse+model+of+dilated+cardiomyopathy+reveals+progressive+intracellular+stress+responses.">Comparative proteomics profiling of a phospholamban mutant mouse model of dilated cardiomyopathy reveals progressive intracellular stress responses.</a> Mol Cell Proteomics. 7, 519-533 (2008).</li><li>Singh, H., Warburton, S., Vondriska, T. M., Khakh, B. S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Proteomics+to+Identify+Proteins+Interacting+with+P2X2+Ligand-Gated+Cation+Channels.">Proteomics to Identify Proteins Interacting with P2X2 Ligand-Gated Cation Channels.</a> J. Vis. Exp. (27), e1178 (2009).</li><li>Park, S. K., Venable, J. D., Xu, T., Yates, J. 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Due metodi principali per isolamento nucleare sono stati esaminati in precedenza: 27 uno è la tecnica di omogeneizzazione Behrens tessuto liofilizzato in un solvente non acquoso e la seconda, una modifica che usiamo qui, omogeneizzazione di tessuto in una soluzione acquosa di saccarosio / soluzione salina seguita dal differenziale o centrifugazione in gradiente di densità. Subfractionation dei nuclei mediante estrazione acido su campioni di tessuto è uno strumento importante pe...

<table border="1"><tbody><tr><td> Nome del reagente </td><td> Azienda </td><td> Numero di catalogo </td></tr><tr><td> Dulbeco Eagle modificato Media </td><td> Invitrogen </td><td> 11965 </td></tr><tr><td> Proteasi pellet </td><td> Roche </td><td> 04 693 159 001 </td></tr><tr><td> 100 um filtro </td><td> BD Falcon </td><td> 352360 </td></tr><tr><td> Ultracut ultramicrotomo </td><td> Reichert </td><td></td></tr><tr><td> 100CX elettronica a trasmissione Microscopio </td><td> JEOL USA, Inc. </td><td></td></tr><tr><td> Rigogolo </td><td> BioRad </td><td> 161-0496 </td></tr><tr><td> Istone H2A anticorpo </td><td> Santa Cruz </td><td> sc-8648 </td></tr><tr><td> Nucleoporin anticorpo p62 </td><td> BD Biosciences </td><td> 610498 </td></tr><tr><td> Adenina nucleomarea trasportatore anticorpo </td><td> Santa Cruz </td><td> sc-9299 </td></tr><tr><td> BiP anticorpo </td><td> Santa Cruz </td><td> sc-1050 </td></tr><tr><td> Tubulina </td><td> Sigma </td><td> T1568 </td></tr><tr><td> Istone H3 anticorpo </td><td> Abcam </td><td> ab1791 </td></tr><tr><td> Fibrillarina anticorpo </td><td> Segnalazione cellulare </td><td> C12C3 </td></tr><tr><td> SNRP70 anticorpo </td><td> Abcam </td><td> ab51266 </td></tr><tr><td> E2F-1 anticorpo </td><td> Thermo Fisher </td><td> MS-879 </td></tr><tr><td> Retinoblastoma anticorpo </td><td> BD Biosciences </td><td> 554136 </td></tr><tr><td> Ipossia inducibile fattore-1 anticorpo </td><td> Novus Biologicals </td><td> NB100-469 </td></tr><tr><td> BCA proteina test </td><td> Thermo Scientific </td><td> 23227 </td></tr><tr><td> Colonna a fase inversa </td><td> Obj Nuovocace </td><td> PFC7515-B14-10 </td></tr><tr><td> Bioworks Browser </td><td> Thermo Scientific </td><td></td></tr></tbody></table>

quantitative analysis of chromatin proteomes in disease

Nel nucleo risiedono le proteomi le cui funzioni sono più intimamente collegati con regolazione genica. Adulto nuclei cardiomiociti mammiferi sono unici a causa della elevata percentuale di cellule binucleated, 1 lo stato prevalentemente eterocromatico del DNA, e la non divisione natura del cardiomiociti che rende nuclei adulto in uno stato permanente di interfase. 2 Regolazione trascrizionale durante lo sviluppo e la malattia sono stati ben studiati in questo organo, 3-5 ma ciò che rimane relativamente inesplorato è il ruolo svolto dalle proteine ​​nucleari responsabili per il confezionamento del DNA e di espressione, e di come queste proteine ​​controllare i cambiamenti nei programmi trascrizionali che si verificano durante la malattia. 6 Nella sviluppato mondo, la malattia di cuore è la causa numero uno di mortalità sia per gli uomini e le donne. 7 Insight su come proteine ​​nucleari cooperano per regolare la progressione di questa malattia è fondamentale per far progredire l&#39;attuale trattamento opzioni. La spettrometria di massa è lo strumento ideale per affrontare queste domande in quanto permette una annotazione imparziale della quantificazione nucleare proteoma e relativo per come l&#39;abbondanza di queste proteine ​​cambiamenti con la malattia. Mentre ci sono stati diversi studi di proteomica per mammiferi complessi proteici nucleari, 8-13 fino a poco tempo 14 vi è stato un solo studio che esamina la proteoma cardiaco nucleare, ed è considerato il nucleo intero, piuttosto che esplorare il proteoma a livello di compartimenti sub nucleari . 15 In gran parte, questa carenza di lavoro è dovuta alla difficoltà di isolare nuclei cardiaca. Nuclei cardiaca avvenire entro un rigido e denso actina-miosina dell&#39;apparecchio a cui sono collegati tramite estensioni multiple dal reticolo endoplasmatico, nella misura in cui altera contrazione miociti loro forma complessiva. 16 Inoltre, cardiomiociti sono il 40% in volume mitocondri 17 che necessitates arricchimento del nucleo parte le altri organelli. Qui si descrive un protocollo per cardiaca arricchimento nucleare e ulteriore frazionamento in compartimenti biologicamente rilevanti. Inoltre, i metodi di dettaglio per l&#39;etichetta senza dissezione massa quantitativa spettrometria di queste frazioni-tecniche suscettibili di sperimentazione in vivo in vari modelli animali e sistemi di organi in cui marcatura metabolica non è fattibile.

Figura 4 mette in evidenza l&#39;utilità di questa forma di quantificazione relativa. Mostrata nel pannello di sinistra sono i singoli picchi peptide monoisotopico (sovrapposto da diversi tipi di mouse), che sono state designate come appartenenti alla proteina HMGB1 (identificato tramite ricerca nel database). Ogni picco, essenzialmente un cromatografo ionico estratta per il dato peptide, proviene da un mouse diverso. I gruppi rappresentano tre diversi stati fisiologici: basale, l&#39;ipertrofia cardia...

Watch this Scientific Journal Video about Quantitative Analysis of Chromatin Proteomes in Disease at JoVE.com

Quantitative Analysis of Chromatin Proteomes in Disease

I progressi nella spettrometria di massa hanno permesso l&#39;analisi ad alta velocità di espressione della proteina e la modifica in una miriade di tessuti. In combinazione con frazionamento subcellulare e modelli di malattia, di massa quantitativa spettrometria e bioinformatica può rivelare nuove proprietà nei sistemi biologici. Il metodo qui descritto analizza cromatina proteine ​​associate alla regolazione di malattie cardiache ed è facilmente applicabile ad altre<em&gt; In vivo</em&gt; Modelli di malattie umane.

Analisi quantitativa di proteomi cromatina nella malattia

In the nucleus reside the proteomes whose functions are most intimately linked with gene regulation. Adult mammalian cardiomyocyte nuclei are unique due ...

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13.0K Views.  David Geffen School of Medicine at UCLA. I progressi nella spettrometria di massa hanno permesso l'analisi ad alta velocità di espressione della proteina e la modifica in una miriade di tessuti. In combinazione con frazionamento subcellulare e modelli di malattia, di massa quantitativa spettrometria e bioinformatica può rivelare nuove proprietà nei sistemi biologici. Il metodo qui descritto analizza cromatina proteine ​​associate alla regolazione di malattie cardiache ed è facilmente applicabile ad a...

Video: Quantitative Analysis of Chromatin Proteomes in Disease

Quantitative Analysis of Cancer Metastasis using an Avian Embryo Model

During metastasis cancer cells disseminate from the primary tumor, invade into surrounding tissues, and spread to distant organs. Metastasis is a complex process that can involve many tissue types, span variable time periods, and often occur deep within organs, making it difficult to investigate and quantify. In addition, the efficacy of the metastatic process is influenced by multiple steps in the metastatic cascade making it difficult to evaluate the contribution of a single aspect of tumor cell behavior. As a consequence, metastasis assays are frequently performed in experimental animals to provide a necessarily realistic context in which to study metastasis. Unfortunately, these models are further complicated by their complex physiology. The chick embryo is a unique in vivo model that overcomes many limitations to studying metastasis, due to the accessibility of the chorioallantoic membrane (CAM), a well-vascularized extra-embryonic tissue located underneath the eggshell that is receptive to the xenografting of tumor cells (figure 1). Moreover, since the chick embryo is naturally immunodeficient, the CAM readily supports the engraftment of both normal and tumor tissues. Most importantly, the avian CAM successfully supports most cancer cell characteristics including growth, invasion, angiogenesis, and remodeling of the microenvironment. This makes the model exceptionally useful for the investigation of the pathways that lead to cancer metastasis and to predict the response of metastatic cancer to new potential therapeutics. The detection of disseminated cells by species-specific Alu PCR makes it possible to quantitatively assess metastasis in organs that are colonized by as few as 25 cells. Using the Human Epidermoid Carcinoma cell line (HEp3) we use this model to analyze spontaneous metastasis of cancer cells to distant organs, including the chick liver and lung. Furthermore, using the Alu-PCR protocol we demonstrate the sensitivity and reproducibility of the assay as a tool to analyze and quantitate intravasation, arrest, extravasation, and colonization as individual elements of metastasis.

Using quantitative PCR, we demonstrate how the well-established chick CAM model can be used to quantitatively analyze the metastasis of human tumor cells to distant organs.

L&#39;analisi quantitativa delle metastasi del cancro utilizzando un modello di aviaria Embryo

During metastasis cancer cells disseminate from the primary tumor, invade into surrounding tissues, and spread to distant organs. Metastasis is a complex ...

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Medicina

A Simple Method of Mouse Lung Intubation

A simple procedure to intubate mice for pulmonary function measurements would have several advantages in longitudinal studies with limited numbers or expensive animal. One of the reasons that this is not done more routinely is that it is relatively difficult, despite there being several published studies that describe ways to achieve it. In this paper we demonstrate a procedure that eliminates one of the major hurdles associated with this intubation, that of visualizing the trachea during the entire time of intubation. The approach uses a 0.5 mm fiberoptic light source that serves as an introducer to direct the intubation cannula into the mouse trachea. We show that it is possible to use this procedure to measure lung mechanics in individual mice over a time course of at least several weeks. The technique can be set up with relatively little expense and expertise, and it can be routinely accomplished with relatively little training. This should make it possible for any laboratory to routinely carry out this intubation, thereby allowing longitudinal studies in individual mice, thereby minimizing the number of mice needed and increasing the statistical power by using each mouse as its own control.

This paper describes a striaghforward and efficient method of intubating mice for pulmonary function measurements or pulmonary instillation, that allows the mice to recover and be studied at later times. The procedure involves an inexpensive fiberoptic light source that directly illuminates the trachea.

Un metodo semplice di intubazione mouse polmone

A  simple procedure to intubate mice for pulmonary function measurements would  have several advantages in longitudinal studies with limited numbers or  ...

Quantitative Analysis and Characterization of Atherosclerotic Lesions in the Murine Aortic Sinus

Atherosclerosis is a disease of the large arteries and a major underlying cause of myocardial infarction and stroke. Several different mouse models have been developed to facilitate the study of the molecular and cellular pathophysiology of this disease. In this manuscript we describe specific techniques for the quantification and characterization of atherosclerotic lesions in the murine aortic sinus and ascending aorta. The advantage of this procedure is that it provides an accurate measurement of the cross-sectional area and total volume of the lesion, which can be used to compare atherosclerotic progression across different treatment groups. This is possible through the use of the valve leaflets as an anatomical landmark, together with careful adjustment of the sectioning angle. We also describe basic staining methods that can be used to begin to characterize atherosclerotic progression. These can be further modified to investigate antigens of specific interest to the researcher. The described techniques are generally applicable to a wide variety of existing and newly created dietary and genetically-induced models of atherogenesis.

We describe procedures to quantify and characterize atherosclerotic lesions in mouse models using precision sectioning of the aortic sinus and ascending aorta combined with histochemical and immunohistochemical analysis.

Atherosclerosis is a disease of the large arteries and a major underlying cause of myocardial infarction and stroke. Several different mouse models have ...

Quantitative Magnetic Resonance Imaging of Skeletal Muscle Disease

Quantitative magnetic resonance imaging (qMRI) describes the development and use of MRI to quantify physical, chemical, and/or biological properties of living systems. Neuromuscular diseases often exhibit a temporally varying, spatially heterogeneous, and multi-faceted pathology. The goal of this protocol is to characterize this pathology using qMRI methods. The MRI acquisition protocol begins with localizer images (used to locate the position of the body and tissue of interest within the MRI system), quality control measurements of relevant magnetic field distributions, and structural imaging for general anatomical characterization. The qMRI portion of the protocol includes measurements of the longitudinal and transverse relaxation time constants (T1 and T2, respectively). Also acquired are diffusion-tensor MRI data, in which water diffusivity is measured and used to infer pathological processes such as edema. Quantitative magnetization transfer imaging is used to characterize the relative tissue content of macromolecular and free water protons. Lastly, fat-water MRI methods are used to characterize fibro-adipose tissue replacement of muscle. In addition to describing the data acquisition and analysis procedures, this paper also discusses the potential problems associated with these methods, the analysis and interpretation of the data, MRI safety, and strategies for artifact reduction and protocol optimization.

Neuromuscular diseases often exhibit a temporally varying, spatially heterogeneous, and multi-faceted pathology. The goal of this protocol is to characterize this pathology using non-invasive magnetic resonance imaging methods.

Quantitative Magnetic Resonance Imaging di malattia muscolare scheletrico

Quantitative magnetic resonance imaging (qMRI) describes the development and use of MRI to quantify physical, chemical, and/or biological properties of ...

Quantitative Mass Spectrometric Profiling of Cancer-cell Proteomes Derived From Liquid and Solid Tumors

In-depth analyses of cancer cell proteomes are needed to elucidate oncogenic pathomechanisms, as well as to identify potential drug targets and diagnostic biomarkers. However, methods for quantitative proteomic characterization of patient-derived tumors and in particular their cellular subpopulations are largely lacking. Here we describe an experimental set-up that allows quantitative analysis of proteomes of cancer cell subpopulations derived from either liquid or solid tumors. This is achieved by combining cellular enrichment strategies with quantitative Super-SILAC-based mass spectrometry followed by bioinformatic data analysis. To enrich specific cellular subsets, liquid tumors are first immunophenotyped by flow cytometry followed by FACS-sorting; for solid tumors, laser-capture microdissection is used to purify specific cellular subpopulations. In a second step, proteins are extracted from the purified cells and subsequently combined with a tumor-specific, SILAC-labeled spike-in standard that enables protein quantification. The resulting protein mixture is subjected to either gel electrophoresis or Filter Aided Sample Preparation (FASP) followed by tryptic digestion. Finally, tryptic peptides are analyzed using a hybrid quadrupole-orbitrap mass spectrometer, and the data obtained are processed with bioinformatic software suites including MaxQuant. By means of the workflow presented here, up to 8,000 proteins can be identified and quantified in patient-derived samples, and the resulting protein expression profiles can be compared among patients to identify diagnostic proteomic signatures or potential drug targets.

In-depth analyses of cancer cell proteomes facilitate identification of novel drug targets and diagnostic biomarkers. We describe an experimental workflow for quantitative analysis of (phospho-)proteomes in cancer cell subpopulations derived from liquid and solid tumors. This is achieved by combining cellular enrichment strategies with quantitative Super-SILAC-based mass spectrometry.

Mass quantitativa spettrometrica Profiling di proteomi cancro-cellulari derivate da liquido e tumori solidi

In-depth analyses of cancer cell proteomes are needed to elucidate oncogenic pathomechanisms, as well as to identify potential drug targets and diagnostic ...

Quantitative 3D In Silico Modeling (q3DISM) of Cerebral Amyloid-beta Phagocytosis in Rodent Models of Alzheimer's Disease

Neuroinflammation is now recognized as a major etiological factor in neurodegenerative disease. Mononuclear phagocytes are innate immune cells responsible for phagocytosis and clearance of debris and detritus. These cells include CNS-resident macrophages known as microglia, and mononuclear phagocytes infiltrating from the periphery. Light microscopy has generally been used to visualize phagocytosis in rodent or human brain specimens. However, qualitative methods have not provided definitive evidence of in vivo phagocytosis. Here, we describe quantitative 3D in silico modeling (q3DISM), a robust method allowing for true 3D quantitation of amyloid-&#946; (A&#946;) phagocytosis by mononuclear phagocytes in rodent Alzheimer&#39;s Disease (AD) models. The method involves fluorescently visualizing A&#946; encapsulated within phagolysosomes in rodent brain sections. Large z-dimensional confocal datasets are then 3D reconstructed for quantitation of A&#946; spatially colocalized within the phagolysosome. We demonstrate the successful application of q3DISM to mouse and rat brains, but this methodology can be extended to virtually any phagocytic event in any tissue.

Quantitative 3D In Silico Modeling q3DISM of Cerebral Amyloid-beta Phagocytosis in Rodent Models of Alzheimer&#039;s Disease

We developed a methodology for quantitative 3D in silico modeling (q3DISM) of cerebral amyloid-&#946; (A&#946;) phagocytosis by mononuclear phagocytes in rodent models of Alzheimer&#39;s disease. This method can be generalized for the quantitation of virtually any phagocytic event in vivo.

3D Quantitative<em&gt; In Silico</em&gt; Modeling (q3DISM) di cerebrale amiloide-beta fagocitosi in roditori modelli di malattia di Alzheimer

Neuroinflammation is now recognized as a major etiological factor in neurodegenerative disease. Mononuclear phagocytes are innate immune cells responsible ...

Application of Granger Causality Analysis of the Directed Functional Connection in Alzheimer's Disease and Mild Cognitive Impairment

Impaired functional connectivity in the Default Mode Network (DMN) may be involved in the progression of Alzheimer&#39;s Disease (AD). The Posterior Cingulate Cortex (PCC) is a potential imaging marker for monitoring the progression of AD. Previous studies did not focus on the functional connectivity between the PCC and nodes in regions outside the DMN, but our study is an effort to explore these overlooked functional connections. For collecting data, we used functional Magnetic Resonance Imaging (fMRI) and Granger Causality Analysis (GCA). fMRI provides a non-invasive method for studying the dynamic interactions between the different brain regions. GCA is a statistical hypothesis test for determining whether one-time series is useful in forecasting another. In simple terms, it is judged by comparing the &#34;Known all the information on the last moment, the distribution of the probability of X at this time&#34; and the &#34;Known all the information on the last moment except Y, the distribution of the probability of X at this time&#34;, to determine whether there is a causal relationship between Y and X. This definition is based on the complete information source and stationary chronological sequence. The main step of this analysis is to use X and Y to establish the regression equation and draw a causal relationship by a hypothetical test. Since GCA can measure causal effects, we used it to investigate the anisotropy of the functional connectivity and explore the hub function of the PCC. Here, we screened 116 participants for MRI scanning, and after preprocessing the data obtained from neuroimaging, we used GCA to derive the causal relationship of each node. Finally, we concluded that the directed connection is significantly different between the Mild Cognitive Impairment (MCI) and AD groups, both from the PCC to the whole brain and from the whole brain to the PCC.

Application of Granger Causality Analysis of the Directed Functional Connection in Alzheimer&#039;s Disease and Mild Cognitive Impairment

Based on resting-state functional magnetic resonance imaging with Granger causality analysis, we investigated the alterations in the directed functional connectivity between the posterior cingulate cortex and whole brain in patients with Alzheimer&#39;s Disease (AD), patients with Mild Cognitive Impairment (MCI), and healthy controls.

Applicazione dell'analisi di causalità di Granger del diretto collegamento funzionale nella malattia di Alzheimer e Mild Cognitive Impairment

Impaired functional connectivity in the Default Mode Network (DMN) may be involved in the progression of Alzheimer&#39;s Disease (AD). The Posterior ...

In Vivo Morphometric Analysis of Human Cranial Nerves Using Magnetic Resonance Imaging in Meni&#232;re's Disease Ears and Normal Hearing Ears

Analysis of neural structures in Meni&#232;re's Disease (MD) is of importance, since a loss of such structures has previously been proposed for this patient group but has yet to be confirmed. This protocol describes a method of in vivo evaluation of neural changes especially well suitable for cranial nerve analysis using magnetic resonance imaging (MRI). MD-patients and normal hearing persons were examined in a 3-T MR-scanner using a scan protocol including strongly T2-weighted 3D gradient-echo-sequence (3D-CISS). In the patient group, MD was additionally confirmed using MRI-based assessment of endolymphatic hydrops. Morphometric analysis was performed using a freeware DICOM viewer. Evaluation of cranial nerves included measurements of cross-sectional areas (CSAs) of the nerves at different levels as well as orthogonal diametric measurements.

&lt;em&gt;In Vivo&lt;/em&gt; Morphometric Analysis of Human Cranial Nerves Using Magnetic Resonance Imaging in Meni&amp;#232;re&#039;s Disease Ears and Normal Hearing Ears

To evaluate morphological changes of cranial nerves such as loss of neural structures or swelling of cranial nerves in Meni&#232;re&#39;s Disease (MD) or in healthy persons in vivo, a protocol of evaluation has been developed using magnetic resonance imaging (MRI). Additional MRI-based confirmation of MD was performed.

In Vivo Analisi morfometrica dei nervi cranici umano usando la formazione immagine a risonanza magnetica in orecchie di malattia di Menière e orecchie udito normale

Analysis of neural structures in Meni&#232;re's Disease (MD) is of importance, since a loss of such structures has previously been proposed for this ...

Whole-brain Segmentation and Change-point Analysis of Anatomical Brain MRI&#8212;Application in Premanifest Huntington's Disease

Recent advances in MRI offer a variety of useful markers to identify neurodegenerative diseases. In Huntington&#39;s disease (HD), regional brain atrophy begins many years prior to the motor onset (during the &#34;premanifest&#34; period), but the spatiotemporal pattern of regional atrophy across the brain has not been fully characterized. Here we demonstrate an online cloud-computing platform, &#34;MRICloud&#34;, which provides atlas-based whole-brain segmentation of T1-weighted images at multiple granularity levels, and thereby, enables us to access the regional features of brain anatomy. We then describe a regression model that detects statistically significant inflection points, at which regional brain atrophy starts to be noticeable, i.e. the &#34;change-point&#34;, with respect to a disease progression index. We used the CAG-age product (CAP) score to index the disease progression in HD patients. Change-point analysis of the volumetric measurements from the segmentation pipeline, therefore, provides important information of the order and pattern of structural atrophy across the brain. The paper illustrates the use of these techniques on T1-weighted MRI data of premanifest HD subjects from a large multicenter PREDICT-HD study. This design potentially has wide applications in a range of neurodegenerative diseases to investigate the dynamic changes of brain anatomy.

Whole-brain Segmentation and Change-point Analysis of Anatomical Brain MRI&amp;#8212;Application in Premanifest Huntington&#039;s Disease

This paper describes a statistical model for volumetric MRI data analysis, which identifies the &#34;change-point&#34; when brain atrophy begins in premanifest Huntington&#39;s disease. Whole-brain mapping of the change-points is achieved based on brain volumes obtained using an atlas-based segmentation pipeline of T1-weighted images.

Segmentazione del intero-cervello e analisi del punto di svolta di anatomico del cervello MRI — applicazione in premanifesti sulla malattia di Huntington

Recent advances in MRI offer a variety of useful markers to identify neurodegenerative diseases. In Huntington&#39;s disease (HD), regional brain atrophy ...

Quantitative [18F]-Naf-PET-MRI Analysis for the Evaluation of Dynamic Bone Turnover in a Patient with Facetogenic Low Back Pain

Imaging techniques that reflect dynamic bone turnover may aid in characterizing a wide range of bone pathologies. Bone is a dynamic tissue undergoing continuous remodeling with the competing activity of osteoblasts, which produce the new bone matrix, and osteoclasts, whose function is to eliminate mineralized bone. [18F]-NaF is a positron emission tomography (PET) radiotracer that enables visualization of bone metabolism. [18F]-NaF is chemically absorbed into hydroxyapatite in the bone matrix by osteoblasts and can thus noninvasively detect osteoblastic activity, which is occult to conventional imaging techniques. Kinetic modeling of dynamic [18F]-NaF-PET data provides detailed quantitative measures of bone metabolism. Conventional semi-quantitative PET data, which utilizes standardized uptake values (SUVs) as a measure of radiotracer activity, is referred to as a static technique due to its snapshot of tracer uptake in time.&#160; Kinetic modeling, however, utilizes dynamic image data where tracer levels are continuously acquired providing tracer uptake temporal resolution. From the kinetic modeling of dynamic data, quantitative values like blood flow and metabolic rate (i.e., potentially informative metrics of tracer dynamics) can be extracted, all with respect to the measured activity in the image data. When combined with dual modality PET-MRI, region-specific kinetic data can be correlated with anatomically registered high-resolution structural and pathologic information afforded by MRI. The goal of this methodological manuscript is to outline detailed techniques for performing and analyzing dynamic [18F]-NaF-PET-MRI data. The lumbar facet joint is a common site of degenerative arthritis disease and a common cause for axial low back pain.&#160; Recent studies suggest [18F]-NaF-PET may serve as a useful biomarker of painful facetogenic disease.&#160; The human lumbar facet joint will, therefore, be used as a prototypical region of interest for dynamic [18F]-NaF-PET-MRI analysis in this manuscript.

Quantitative [18F]-Naf-PET-MRI Analysis for the Evaluation of Dynamic Bone Turnover in a Patient with Facetogenic Low Back Pain

Imaging techniques that reflect dynamic bone turnover may aid in characterizing a wide range of bone pathologies. We present detailed methodologies for performing and analyzing dynamic [18F]-NaF-PET-MRI data in a patient with facetogenic low back pain using the lumbar facet joints as a prototypical region of interest.

Quantitativo [18F]-Naf-PET-MRI Analisi per la valutazione del fatturato osseo dinamico in un paziente con mal di schiena facetogenico

Imaging techniques that reflect dynamic bone turnover may aid in characterizing a wide range of bone pathologies. Bone is a dynamic tissue undergoing ...