Vondriska 연구소는 NIH와 UCLA에서 Laubisch 기금의 국립 심장 폐 혈액 연구소에서 보조금에 의해 지원됩니다. EM은 UCLA의 생리학에서 제니퍼 S. Buchwald 대학원 동지의 수상자이다, HC는 미국 심장 협회 사전 박사의 원정이 수상자이다, MP는 NIH 루스 Kirschstein 포스트 박사의 원정이받는 것입니다, 그리고 SF가의 수신자입니다 NIH K99 수상.

<ol>
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관심 없음 충돌이 선언 없습니다.

핵 절연을위한 두 가지 방법은 이미 검토 한 : 27 하나는 수성 자당 / 소금 용액에 조직을 homogenizing의 비 수성 용매에 동결 건조 된 조직을 homogenizing의 Behrens 기술과 두 번째, 우리가 사용하는의 수정입니다 다음 차동 또는 밀도 기울기 원심 분리에 의해. 조직 샘플에서 산 추출하여 핵의 Subfractionation은 histones을 분석되는 원본을 목표로 1960, 28 년부터 사용 된 ?...

<table border="1"><tbody><tr><td> 시약의 이름 </td><td> 회사 </td><td> 카탈로그 번호 </td></tr><tr><td> Dulbeco 수정일 독수리 중 </td><td> Invitrogen </td><td> 11,965 </td></tr><tr><td> 단백 분해 효소 펠릿 </td><td> 로슈 </td><td> 04 693 159 001 </td></tr><tr><td> 100 μm의 스트레이너 </td><td> BD 팔콘 </td><td> 352360 </td></tr><tr><td> Ultracut ultramicrotome </td><td> 라이 </td><td></td></tr><tr><td> 100CX 전송 전자 현미경 </td><td> JEOL USA 주식회사 </td><td></td></tr><tr><td> 꾀꼬리 </td><td> BioRad </td><td> 161-0496 </td></tr><tr><td> 히스톤 H2A 항체 </td><td> 산타 크루즈 (Santa Cruz) </td><td> SC-8648 </td></tr><tr><td> Nucleoporin p62 항체 </td><td> BD Biosciences </td><td> 610498 </td></tr><tr><td> 아데닌 nucleo조수 전송 항체 </td><td> 산타 크루즈 (Santa Cruz) </td><td> SC-9299 </td></tr><tr><td> bip (빨리) 항체 </td><td> 산타 크루즈 (Santa Cruz) </td><td> SC-1050 </td></tr><tr><td> Tubulin 항체 </td><td> 시그마 </td><td> T1568 </td></tr><tr><td> 히스톤 H3 항체 </td><td> Abcam </td><td> ab1791 </td></tr><tr><td> Fibrillarin 항체 </td><td> 셀 시그널링 </td><td> C12C3 </td></tr><tr><td> SNRP70 항체 </td><td> Abcam </td><td> ab51266 </td></tr><tr><td> E2F-1 항체 </td><td> 온도 피셔 </td><td> MS-879 </td></tr><tr><td> Retinoblastoma 항체 </td><td> BD Biosciences </td><td> 554136 </td></tr><tr><td> Hypoxia inducible 인자-1 항체 </td><td> Novus 체액 </td><td> NB100-469 </td></tr><tr><td> BCA 단백질 분석 </td><td> 열 과학 </td><td> 23,227 </td></tr><tr><td> 위상 열을 역방향 </td><td> 새로운 OBJective </td><td> PFC7515-B14-10 </td></tr><tr><td> BioWorks 브라우저 </td><td> 열 과학 </td><td></td></tr></tbody></table>

quantitative analysis of chromatin proteomes in disease

핵에서 누구 기능을 가장 밀접한 유전자 조절과 연결되어 proteomes을 거주. 성인 포유류의 cardiomyocyte의 핵은 binucleated 세포의 높은 비율 1 DNA의 주로 heterochromatic 상태에 따라 고유하며, 개발 중에 계면의 영구적 인 상태에 성인 핵을 렌더링 cardiomyocyte의 비 분리 자연 2. 전사 규제와 병은 잘이 단백질이 질병 중에 발생하는 전사 프로그램에 변경 사항을 관리하는 방법이 오르간, 3-5에서 공부하지만 상대적으로 미개척 남아는 DNA 포장과 표현에 대한 책임 핵 단백질에 의해 연주 역할이며,되었습니다. 6 개발에 세계 심장 질환은 남성과 여성 모두에 대한 사망률의 최고의 원인입니다. 핵 단백질이 질병의 진행을 조절하는 협력 방법에 대한 일곱 통계는 현재 치료 O를 발전을위한 중요합니다습니. 질량 분석법은 병 어떻게 이러한 단백질의 풍부한 변경을위한 핵 프로테옴와 상대 정량화의 편견 주석을 허용 이러한 문제에 대응을위한 이상적인 도구입니다. 최근까지 포유류의 핵 단백질 단지, 8-13 여러 proteomic 연구가되어있는 동안 14 심장 핵 프로테옴을 검토 한 연구가있다, 그것은 오히려 핵 하위 구획의 수준에서 프로테옴을 둘러보다, 전체 핵을 고려 . 많은 부분에서 15 일이 부족 심장 핵을 분리의 어려움 때문입니다. 심장 핵은 myocyte 수축 변하는 자신의 전반적인 모양. 16 또한, cardiomyocytes 볼륨 17으로 40% 미토콘드리아이라는 범위, 그들이 endoplasmic 소포체에서 여러 확장을 통해 연결되어 할 수있는 강성과 고밀도 고를 - 마이 오신 장치에서 발생하는 necessita다른 세포 소기관에서 떨어져 핵의 농축 tes. 여기 생물학적 관련성이 높은 구획에 심장 핵 농축 및 추가 분류를위한 프로토콜을 설명합니다. 또한, 신진 대사 라벨이 적합하지 않을 다양한 동물 모델 및 장기 시스템의 생체 실험의 의무 분수는 - 기술의 라벨이없는 정량 대량 spectrometric 해부에 대한 세부 방법.

그림 4는 상대적으로 정량화이 양식의 유틸리티를 강조 표시합니다. 왼쪽 패널에 표시되는 단백질 HMGB1 (데이터베이스 검색을 통해 확인)에 속하는 것으로 지정되어 개별 monoisotopic 펩타이드 피크는 (다른 마우스에서 중첩)입니다. 각 피크는 기본적으로 주어진 펩타이드에 대한 추출 이온 크로마토 그래프는 다른 마우스에서 제공합니다. 세 생물 각 그룹에 대한 복제와 함께 기초, 심?...

Watch this Scientific Journal Video about Quantitative Analysis of Chromatin Proteomes in Disease at JoVE.com

Quantitative Analysis of Chromatin Proteomes in Disease

질량 분광법의 발전은 조직의 호스트에 단백질 표현 및 수정의 높은 처리량 분석을 허용하고 있습니다. subcellular 분류 및 질병 모델, 분광 및 생물 정보학은 생물 시스템에 새로운 속성을 표시 할 수 있습니다 양적 질량과 결합. 여기에 설명 된 방법은 심장 질환의 설정에 염색질 - 관련 단백질을 분석하고 다른 사람에게 쉽게 적용 할 수 있습니다<em&gt; 생체 내</em&gt; 인간의 질병의 모델.

질병의 염색질 Proteomes의 정량 분석

In the nucleus reside the proteomes whose functions are most intimately linked with gene regulation. Adult mammalian cardiomyocyte nuclei are unique due ...

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13.0K 조회수.  David Geffen School of Medicine at UCLA. 질량 분광법의 발전은 조직의 호스트에 단백질 표현 및 수정의 높은 처리량 분석을 허용하고 있습니다. subcellular 분류 및 질병 모델, 분광 및 생물 정보학은 생물 시스템에 새로운 속성을 표시 할 수 있습니다 양적 질량과 결합. 여기에 설명 된 방법은 심장 질환의 설정에 염색질 - 관련 단백질을 분석하고 다른 사람에게 쉽게 적용 할 수 있습니다 생체 내

비디오: Quantitative Analysis of Chromatin Proteomes in Disease

Quantitative Analysis of Cancer Metastasis using an Avian Embryo Model

During metastasis cancer cells disseminate from the primary tumor, invade into surrounding tissues, and spread to distant organs. Metastasis is a complex process that can involve many tissue types, span variable time periods, and often occur deep within organs, making it difficult to investigate and quantify. In addition, the efficacy of the metastatic process is influenced by multiple steps in the metastatic cascade making it difficult to evaluate the contribution of a single aspect of tumor cell behavior. As a consequence, metastasis assays are frequently performed in experimental animals to provide a necessarily realistic context in which to study metastasis. Unfortunately, these models are further complicated by their complex physiology. The chick embryo is a unique in vivo model that overcomes many limitations to studying metastasis, due to the accessibility of the chorioallantoic membrane (CAM), a well-vascularized extra-embryonic tissue located underneath the eggshell that is receptive to the xenografting of tumor cells (figure 1). Moreover, since the chick embryo is naturally immunodeficient, the CAM readily supports the engraftment of both normal and tumor tissues. Most importantly, the avian CAM successfully supports most cancer cell characteristics including growth, invasion, angiogenesis, and remodeling of the microenvironment. This makes the model exceptionally useful for the investigation of the pathways that lead to cancer metastasis and to predict the response of metastatic cancer to new potential therapeutics. The detection of disseminated cells by species-specific Alu PCR makes it possible to quantitatively assess metastasis in organs that are colonized by as few as 25 cells. Using the Human Epidermoid Carcinoma cell line (HEp3) we use this model to analyze spontaneous metastasis of cancer cells to distant organs, including the chick liver and lung. Furthermore, using the Alu-PCR protocol we demonstrate the sensitivity and reproducibility of the assay as a tool to analyze and quantitate intravasation, arrest, extravasation, and colonization as individual elements of metastasis.

Using quantitative PCR, we demonstrate how the well-established chick CAM model can be used to quantitatively analyze the metastasis of human tumor cells to distant organs.

조류 엠브료 모델을 사용하여 암 전이의 정량 분석

During metastasis cancer cells disseminate from the primary tumor, invade into surrounding tissues, and spread to distant organs. Metastasis is a complex ...

연구

의학

A Simple Method of Mouse Lung Intubation

A simple procedure to intubate mice for pulmonary function measurements would have several advantages in longitudinal studies with limited numbers or expensive animal. One of the reasons that this is not done more routinely is that it is relatively difficult, despite there being several published studies that describe ways to achieve it. In this paper we demonstrate a procedure that eliminates one of the major hurdles associated with this intubation, that of visualizing the trachea during the entire time of intubation. The approach uses a 0.5 mm fiberoptic light source that serves as an introducer to direct the intubation cannula into the mouse trachea. We show that it is possible to use this procedure to measure lung mechanics in individual mice over a time course of at least several weeks. The technique can be set up with relatively little expense and expertise, and it can be routinely accomplished with relatively little training. This should make it possible for any laboratory to routinely carry out this intubation, thereby allowing longitudinal studies in individual mice, thereby minimizing the number of mice needed and increasing the statistical power by using each mouse as its own control.

This paper describes a striaghforward and efficient method of intubating mice for pulmonary function measurements or pulmonary instillation, that allows the mice to recover and be studied at later times. The procedure involves an inexpensive fiberoptic light source that directly illuminates the trachea.

마우스 폐 삽관 법의 간단한 방법

A  simple procedure to intubate mice for pulmonary function measurements would  have several advantages in longitudinal studies with limited numbers or  ...

Quantitative Analysis and Characterization of Atherosclerotic Lesions in the Murine Aortic Sinus

Atherosclerosis is a disease of the large arteries and a major underlying cause of myocardial infarction and stroke. Several different mouse models have been developed to facilitate the study of the molecular and cellular pathophysiology of this disease. In this manuscript we describe specific techniques for the quantification and characterization of atherosclerotic lesions in the murine aortic sinus and ascending aorta. The advantage of this procedure is that it provides an accurate measurement of the cross-sectional area and total volume of the lesion, which can be used to compare atherosclerotic progression across different treatment groups. This is possible through the use of the valve leaflets as an anatomical landmark, together with careful adjustment of the sectioning angle. We also describe basic staining methods that can be used to begin to characterize atherosclerotic progression. These can be further modified to investigate antigens of specific interest to the researcher. The described techniques are generally applicable to a wide variety of existing and newly created dietary and genetically-induced models of atherogenesis.

We describe procedures to quantify and characterize atherosclerotic lesions in mouse models using precision sectioning of the aortic sinus and ascending aorta combined with histochemical and immunohistochemical analysis.

쥐의 대동맥 부비동의 동맥 경화 병변의 정량 분석​​ 및 특성

Atherosclerosis is a disease of the large arteries and a major underlying cause of myocardial infarction and stroke. Several different mouse models have ...

Carotid Artery Infusions for Pharmacokinetic and Pharmacodynamic Analysis of Taxanes in Mice

When proposing the use of a drug, drug combination, or drug delivery into a novel system, one must assess the pharmacokinetics of the drug in the study model. As the use of mouse models are often a vital step in preclinical drug discovery and drug development1-8, it is necessary to design a system to introduce drugs into mice in a uniform, reproducible manner. Ideally, the system should permit the collection of blood samples at regular intervals over a set time course. The ability to measure drug concentrations by mass-spectrometry, has allowed investigators to follow the changes in plasma drug levels over time in individual mice1, 9, 10. In this study, paclitaxel was introduced into transgenic mice as a continuous arterial infusion over three hours, while blood samples were simultaneously taken by retro-orbital bleeds at set time points. Carotid artery infusions are a potential alternative to jugular vein infusions, when factors such as mammary tumors or other obstructions make jugular infusions impractical. Using this technique, paclitaxel concentrations in plasma and tissue achieved similar levels as compared to jugular infusion. In this tutorial, we will demonstrate how to successfully catheterize the carotid artery by preparing an optimized catheter for the individual mouse model, then show how to insert and secure the catheter into the mouse carotid artery, thread the end of the catheter out through the back of the mouse&#8217;s neck, and hook the mouse to a pump to deliver a controlled rate of drug influx. Multiple low volume retro-orbital bleeds allow for analysis of plasma drug concentrations over time.

This method was developed with the goal of delivering a steady drug solution via the carotid artery, to assess the pharmacokinetics of novel drugs in mouse models.

When proposing the use of a drug, drug combination, or drug delivery into a novel system, one must assess the pharmacokinetics of the drug in the study ...

Quantitative Magnetic Resonance Imaging of Skeletal Muscle Disease

Quantitative magnetic resonance imaging (qMRI) describes the development and use of MRI to quantify physical, chemical, and/or biological properties of living systems. Neuromuscular diseases often exhibit a temporally varying, spatially heterogeneous, and multi-faceted pathology. The goal of this protocol is to characterize this pathology using qMRI methods. The MRI acquisition protocol begins with localizer images (used to locate the position of the body and tissue of interest within the MRI system), quality control measurements of relevant magnetic field distributions, and structural imaging for general anatomical characterization. The qMRI portion of the protocol includes measurements of the longitudinal and transverse relaxation time constants (T1 and T2, respectively). Also acquired are diffusion-tensor MRI data, in which water diffusivity is measured and used to infer pathological processes such as edema. Quantitative magnetization transfer imaging is used to characterize the relative tissue content of macromolecular and free water protons. Lastly, fat-water MRI methods are used to characterize fibro-adipose tissue replacement of muscle. In addition to describing the data acquisition and analysis procedures, this paper also discusses the potential problems associated with these methods, the analysis and interpretation of the data, MRI safety, and strategies for artifact reduction and protocol optimization.

Neuromuscular diseases often exhibit a temporally varying, spatially heterogeneous, and multi-faceted pathology. The goal of this protocol is to characterize this pathology using non-invasive magnetic resonance imaging methods.

골격근 질환의 정량적 자기 공명 영상

Quantitative magnetic resonance imaging (qMRI) describes the development and use of MRI to quantify physical, chemical, and/or biological properties of ...

Quantitative Mass Spectrometric Profiling of Cancer-cell Proteomes Derived From Liquid and Solid Tumors

In-depth analyses of cancer cell proteomes are needed to elucidate oncogenic pathomechanisms, as well as to identify potential drug targets and diagnostic biomarkers. However, methods for quantitative proteomic characterization of patient-derived tumors and in particular their cellular subpopulations are largely lacking. Here we describe an experimental set-up that allows quantitative analysis of proteomes of cancer cell subpopulations derived from either liquid or solid tumors. This is achieved by combining cellular enrichment strategies with quantitative Super-SILAC-based mass spectrometry followed by bioinformatic data analysis. To enrich specific cellular subsets, liquid tumors are first immunophenotyped by flow cytometry followed by FACS-sorting; for solid tumors, laser-capture microdissection is used to purify specific cellular subpopulations. In a second step, proteins are extracted from the purified cells and subsequently combined with a tumor-specific, SILAC-labeled spike-in standard that enables protein quantification. The resulting protein mixture is subjected to either gel electrophoresis or Filter Aided Sample Preparation (FASP) followed by tryptic digestion. Finally, tryptic peptides are analyzed using a hybrid quadrupole-orbitrap mass spectrometer, and the data obtained are processed with bioinformatic software suites including MaxQuant. By means of the workflow presented here, up to 8,000 proteins can be identified and quantified in patient-derived samples, and the resulting protein expression profiles can be compared among patients to identify diagnostic proteomic signatures or potential drug targets.

In-depth analyses of cancer cell proteomes facilitate identification of novel drug targets and diagnostic biomarkers. We describe an experimental workflow for quantitative analysis of (phospho-)proteomes in cancer cell subpopulations derived from liquid and solid tumors. This is achieved by combining cellular enrichment strategies with quantitative Super-SILAC-based mass spectrometry.

액체 및 고체 종양 유래의 암세포 프로테옴 정량적 질량 분석 프로파일

In-depth analyses of cancer cell proteomes are needed to elucidate oncogenic pathomechanisms, as well as to identify potential drug targets and diagnostic ...

Quantitative 3D In Silico Modeling (q3DISM) of Cerebral Amyloid-beta Phagocytosis in Rodent Models of Alzheimer's Disease

Neuroinflammation is now recognized as a major etiological factor in neurodegenerative disease. Mononuclear phagocytes are innate immune cells responsible for phagocytosis and clearance of debris and detritus. These cells include CNS-resident macrophages known as microglia, and mononuclear phagocytes infiltrating from the periphery. Light microscopy has generally been used to visualize phagocytosis in rodent or human brain specimens. However, qualitative methods have not provided definitive evidence of in vivo phagocytosis. Here, we describe quantitative 3D in silico modeling (q3DISM), a robust method allowing for true 3D quantitation of amyloid-&#946; (A&#946;) phagocytosis by mononuclear phagocytes in rodent Alzheimer&#39;s Disease (AD) models. The method involves fluorescently visualizing A&#946; encapsulated within phagolysosomes in rodent brain sections. Large z-dimensional confocal datasets are then 3D reconstructed for quantitation of A&#946; spatially colocalized within the phagolysosome. We demonstrate the successful application of q3DISM to mouse and rat brains, but this methodology can be extended to virtually any phagocytic event in any tissue.

Quantitative 3D In Silico Modeling q3DISM of Cerebral Amyloid-beta Phagocytosis in Rodent Models of Alzheimer's Disease

We developed a methodology for quantitative 3D in silico modeling (q3DISM) of cerebral amyloid-&#946; (A&#946;) phagocytosis by mononuclear phagocytes in rodent models of Alzheimer&#39;s disease. This method can be generalized for the quantitation of virtually any phagocytic event in vivo.

양적 3D<em&gt; 인 실리코</em알츠하이머 병의 쥐 모델에서 뇌 아밀로이드 - 베타 탐식&gt; 모델링 (q3DISM)

Neuroinflammation is now recognized as a major etiological factor in neurodegenerative disease. Mononuclear phagocytes are innate immune cells responsible ...

Application of Granger Causality Analysis of the Directed Functional Connection in Alzheimer's Disease and Mild Cognitive Impairment

Impaired functional connectivity in the Default Mode Network (DMN) may be involved in the progression of Alzheimer&#39;s Disease (AD). The Posterior Cingulate Cortex (PCC) is a potential imaging marker for monitoring the progression of AD. Previous studies did not focus on the functional connectivity between the PCC and nodes in regions outside the DMN, but our study is an effort to explore these overlooked functional connections. For collecting data, we used functional Magnetic Resonance Imaging (fMRI) and Granger Causality Analysis (GCA). fMRI provides a non-invasive method for studying the dynamic interactions between the different brain regions. GCA is a statistical hypothesis test for determining whether one-time series is useful in forecasting another. In simple terms, it is judged by comparing the &#34;Known all the information on the last moment, the distribution of the probability of X at this time&#34; and the &#34;Known all the information on the last moment except Y, the distribution of the probability of X at this time&#34;, to determine whether there is a causal relationship between Y and X. This definition is based on the complete information source and stationary chronological sequence. The main step of this analysis is to use X and Y to establish the regression equation and draw a causal relationship by a hypothetical test. Since GCA can measure causal effects, we used it to investigate the anisotropy of the functional connectivity and explore the hub function of the PCC. Here, we screened 116 participants for MRI scanning, and after preprocessing the data obtained from neuroimaging, we used GCA to derive the causal relationship of each node. Finally, we concluded that the directed connection is significantly different between the Mild Cognitive Impairment (MCI) and AD groups, both from the PCC to the whole brain and from the whole brain to the PCC.

Based on resting-state functional magnetic resonance imaging with Granger causality analysis, we investigated the alterations in the directed functional connectivity between the posterior cingulate cortex and whole brain in patients with Alzheimer&#39;s Disease (AD), patients with Mild Cognitive Impairment (MCI), and healthy controls.

알 츠 하이 머 질환과 경미한 인지 장애에 감독된 기능 연결의 그 랜 저 인과관계 분석의 응용 프로그램

Impaired functional connectivity in the Default Mode Network (DMN) may be involved in the progression of Alzheimer&#39;s Disease (AD). The Posterior ...

In Vivo Morphometric Analysis of Human Cranial Nerves Using Magnetic Resonance Imaging in Meni&#232;re's Disease Ears and Normal Hearing Ears

Analysis of neural structures in Meni&#232;re's Disease (MD) is of importance, since a loss of such structures has previously been proposed for this patient group but has yet to be confirmed. This protocol describes a method of in vivo evaluation of neural changes especially well suitable for cranial nerve analysis using magnetic resonance imaging (MRI). MD-patients and normal hearing persons were examined in a 3-T MR-scanner using a scan protocol including strongly T2-weighted 3D gradient-echo-sequence (3D-CISS). In the patient group, MD was additionally confirmed using MRI-based assessment of endolymphatic hydrops. Morphometric analysis was performed using a freeware DICOM viewer. Evaluation of cranial nerves included measurements of cross-sectional areas (CSAs) of the nerves at different levels as well as orthogonal diametric measurements.

To evaluate morphological changes of cranial nerves such as loss of neural structures or swelling of cranial nerves in Meni&#232;re&#39;s Disease (MD) or in healthy persons in vivo, a protocol of evaluation has been developed using magnetic resonance imaging (MRI). Additional MRI-based confirmation of MD was performed.

Vivo에서 Menière의 질병 귀 그리고 정상 청력 귀에 자기 공명 화상 진 찰을 사용 하 여 인간의 두개골 신경의 형태학 분석

Analysis of neural structures in Meni&#232;re's Disease (MD) is of importance, since a loss of such structures has previously been proposed for this ...

Whole-brain Segmentation and Change-point Analysis of Anatomical Brain MRI&#8212;Application in Premanifest Huntington's Disease

Recent advances in MRI offer a variety of useful markers to identify neurodegenerative diseases. In Huntington&#39;s disease (HD), regional brain atrophy begins many years prior to the motor onset (during the &#34;premanifest&#34; period), but the spatiotemporal pattern of regional atrophy across the brain has not been fully characterized. Here we demonstrate an online cloud-computing platform, &#34;MRICloud&#34;, which provides atlas-based whole-brain segmentation of T1-weighted images at multiple granularity levels, and thereby, enables us to access the regional features of brain anatomy. We then describe a regression model that detects statistically significant inflection points, at which regional brain atrophy starts to be noticeable, i.e. the &#34;change-point&#34;, with respect to a disease progression index. We used the CAG-age product (CAP) score to index the disease progression in HD patients. Change-point analysis of the volumetric measurements from the segmentation pipeline, therefore, provides important information of the order and pattern of structural atrophy across the brain. The paper illustrates the use of these techniques on T1-weighted MRI data of premanifest HD subjects from a large multicenter PREDICT-HD study. This design potentially has wide applications in a range of neurodegenerative diseases to investigate the dynamic changes of brain anatomy.

This paper describes a statistical model for volumetric MRI data analysis, which identifies the &#34;change-point&#34; when brain atrophy begins in premanifest Huntington&#39;s disease. Whole-brain mapping of the change-points is achieved based on brain volumes obtained using an atlas-based segmentation pipeline of T1-weighted images.

전체 두뇌 세분화 및 해 부 뇌 MRI의 변화 포인트 분석-Premanifest Huntington의 질병에 응용

Recent advances in MRI offer a variety of useful markers to identify neurodegenerative diseases. In Huntington&#39;s disease (HD), regional brain atrophy ...