Preparation of Tissue Slices for Microdissection and Sample Lysis
3:20
Sample Processing
4:51
Quantification of the Peptides
5:38
Fractionation of the Lys-C and Tryptic Peptides
7:29
Results: Peptide Quantification
8:21
Conclusion
Archival formalin fixed and paraffin embedded (FFPE) clinical samples are valuable material for investigation of diseases. Here we demonstrate a sample preparation workflow allowing in-depth proteomic analysis of microdissected FFPE tissue.