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Isolation and Flow Cytometric Analysis of Human Endocervical Gamma Delta T Cells

DOI :

10.3791/55038-v

February 6th, 2017

February 6th, 2017

8,579 Views

1Department of Microbiology and Immunology, Miller School of Medicine, University of Miami, 2Division of Infectious Diseases, Miller School of Medicine, University of Miami

We describe here an isolation method to obtain human endocervical intraepithelial lymphocytes for the analysis of intraepithelial gamma delta T cells. This protocol can be extended for the purification of endocervical gamma delta T cells by magnetic beads or by cell sorting.

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