First Round of Biopanning: Screening Phage Clones that Bind to the Extracellular Domain of FGFR2
2:26
Titer Determination of the Eluted Phages
3:28
Phage Amplification
5:38
Results: Screening and Identification of Small Peptides Targeting Fibroblast Growth Factor Receptor2
6:38
Conclusion
Trascrizione
Compared with other methods, this protocol is easier to find out new antagonisters, agonisteres, and allosteric modulators quantitatively and qualitatively without knowing the hydro-dilution structure and formation of the target protein. This prot
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Herein, we present a detailed protocol for screening small peptides that bind to FGFR2 using a phage display peptide library. We further analyze the affinity of the selected peptides toward FGFR2 in vitro and its ability to suppress cell proliferation.