Our challenge is to test ingredients'cytotoxicity prior to cosmetic or medical use. The commercial 3-D skin models limited to around three cell types don't reflect real skin complexity. And skin has diverse cell types with essential functions, posing a challenge in accurately assessing ingredient impact.
Our findings indicate that the limiting cell adhesion method produced more well-shaped spheres compared to the commonly-used methods. Additionally, it demands less manipulation, ensuring greater sphere stability. In our skin equivalent, we use up to four types of skin cells, unlike the commercial models that typically incorporate a maximum of three cell types.
Common to these cell models lack accuracy in representing real interaction. While commercial 3-D skin equivalents offer better tissue representation, their cost limits widespread use. Consequently, our self-established models allow for initial cost-effective experiments on a more realistic platform.
Our protocols outline affordable, straightforward ways to create customized 3-D skin models, encompassing spheres and equivalents. In contrast to commercial options, our models offer adjustable appearance and composition tailored to the study's requirements. The method is accessible, not demanding specialized skills or equipment, making them replicable even by novice researchers.
Our aim is to highlight the simplicity of creating various 3-D cellular models, while emphasizing the value of certified commercial models in research. We stress the significance of using realistic models, such as spheres and equivalents. Showcasing how the cells make models, can aid in pre-selecting the testing conditions before employing certified models.
