1。マウスの麻酔の手順<ol><li>動物は、毎分0.5リットル（気化器制御）の流量で、自分のケージから取り出し、5％イソフルランおよび100％酸素でガスの誘導のための麻酔術前誘導のチャンバー内に配置されます。 </li><li>マウスは、麻酔の誘導チャンバ内鎮静チャンバーから除去し、髪の毛の脱毛は、（1分）使用されている場合、腹側腹部を電気バリカンで剃毛、またはチャンバー内に保持されています。 </li><li>まだ鎮静下で、彼らの重量を測定している間、目がイソフルランと酸素を使用して、ノーズコーンと、滅菌眼軟膏が塗布してから、全身麻酔下での加熱装置を循環する暖かい水で背recumbancyに配置されます。この時点で、酸素の割合は、毎分0.2リットルに削減されます。 </li><li>腹部はクロルヘキシジン液（クロルヘキシジン二酢酸は急速にbacterialcidalと持続性がある）を噴霧し、滅菌ガーゼで拭いて三回か手術部位はきれいになるまでさ。 </li></ol> 2。マウスマイクロ外科および回復手順<ol><li>腹側正中切開（2cmは）腹部を露出皮膚や腹壁を通して虹彩をハサミで作られています。滅菌生理食塩水に浸した2x2inのガーゼは、動物の左側に腸を反映するために使用されます。マウスの制止する人は、下大静脈（IVC）の可視化を可能にする場所に置かれている。 </li><li>この時点で、0.2リットル/分の2％isofluranceと100％の酸素率のメンテナンスレベルは、外科的処置の残りのために使用されます。 </li><li>鈍的切開は、滅菌アプリケーター綿棒や余分な繊細な虹彩の半分の湾曲した組織鉗子を用いて促進される。ケアは、彼らは非常に壊れやすいために、マウス組織の取り扱いには注意が必要です。腹腔内に入ると、このようにIVCの可視化を可能にする、滅菌ドレープの上に腸を反映して体外に出すために無菌の生理食塩水に浸した4x4inのガーゼを使用してください。 </li><li>側枝が7から0、非反応性prolene縫合糸で連結している間に腎静脈から腸骨分岐へのすべてのIVCバック枝は、、焼灼されています。 </li><li> IVCは、腎静脈にだけ劣っ、大動脈から分離されている。 7から0 proleneはIVCを連結するために使用されます。 </li><li>開腹部位は、2層方式で閉じられます。当研究室では、腹壁を閉じるには、連続的なパターンで5〜0 vicryl、合成縫合糸を、使用しています。接着剤の皮膚接着剤は、皮膚を閉じるために使用されます。除去するための外付縫合糸はありません。 </li><li>マウスはその後、個々のマウスのケージ中に回収され、加熱ランプ（最小距離 - 24インチ離れてケージから）下（2時間まで45分）手術後に観察された、その後、元の住宅に戻った。それは研究目的に干渉していない場合の鎮痛薬と抗炎症剤を用いることができる。科学的な鎮痛剤を差し控えることを正当化、獣医、そしてICUCAの承認が必要とされています。当研究室では、ブプレノルフィン、0.05〜0.1 mg / kgのSQは、マウスのための優秀なプリと術後の鎮痛剤であることを見出した。全身鎮痛薬は、実験結果を混乱させる可能性がある場合に加えて、4mg/kgでのリドカインは、（0.4 mlの1％溶液の/ kg）の切開部位に注入することができます。 </li><li> IVCあるいは排水枝を結紮または焼灼されていない場合を除き、各実験群から偽の動物は、外科的アプローチのために使用される上記の解剖​​を受ける。 </li><li>安楽死は、げっ歯類のための安楽死についてアメリカ獣医学協会のガイドラインで定められている勧告に従って行われます。 </li></ol>

<ol>
	<li>Naess, I. A., Christiansen, S. C., Romundstad, P., Cannegieter, S. C., Rosendaal, F. R., Hammerstrom, J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Incidence+and+mortality+of+venous+thrombosis:+a+population-based+study.">Incidence and mortality of venous thrombosis: a population-based study.</a> J Thromb Haemost. 5, 692-699 (2007).</li><li>White, R. H., Keenan, C. R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Effects+of+race+and+ethnicity+on+the+incidence+of+venous+thromboembolism.">Effects of race and ethnicity on the incidence of venous thromboembolism.</a> Thromb Res. 123, S11-S17 (2009).</li><li>Heit, J. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+epidemiology+of+venous+thromboembolism+in+the+community.">The epidemiology of venous thromboembolism in the community.</a> Arterioscler Thromb Vasc Biol. 28, 370-372 (2008).</li><li>Henke, P. K., Varga, A., De, S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Deep+vein+thrombosis+resolution+is+modulated+by+monocyte+CXCR2-mediated+activity+in+a+mouse+model.">Deep vein thrombosis resolution is modulated by monocyte CXCR2-mediated activity in a mouse model.</a> Arterioscler Thromb Vasc Biol. 24, 1130-1137 (2004).</li><li>McDonald, A. P., Meier, T. R., Hawley, A. E. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Aging+is+associated+with+impaired+thrombus+resolution+in+a+mouse+model+of+stasis+induced+thrombosis.">Aging is associated with impaired thrombus resolution in a mouse model of stasis induced thrombosis.</a> Thromb Res. 125, 72-78 (2010).</li><li>Myers, D., Farris, D., Hawley, A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Selectins+influence+thrombosis+in+a+mouse+model+of+experimental+deep+venous+thrombosis.">Selectins influence thrombosis in a mouse model of experimental deep venous thrombosis.</a> J Surg Res. 108, 212-221 (2002).</li><li>Myers, D. D., Hawley, A. E., Farris, D. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=P-selectin+and+leukocyte+microparticles+are+associated+with+venous+thrombogenesis.">P-selectin and leukocyte microparticles are associated with venous thrombogenesis.</a> J Vasc Surg. 38, 1075-1089 (2003).</li><li>Myers, D. D. J. r., Rectenwald, J. E., Bedard, P. W. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Decreased+venous+thrombosis+with+an+oral+inhibitor+of+P+selectin.">Decreased venous thrombosis with an oral inhibitor of P selectin.</a> J Vasc Surg. 42, 329-336 (2005).</li></ol>

利害の衝突は宣言されません。

下大静脈血栓症のマウス完全な停滞のモデルは、さまざまな年齢のマウスの複数の株に行うことができます。モデルは、最も重量の少なくとも20グラムのマウスに適しています。静脈血栓症のこの動物モデルは、研究者が静脈の壁と閉塞性静脈血栓の間に炎症性の相互作用を研究することができます。我々の経験から、我々は各群10匹以上の動物が血栓重み、静脈壁の炎症細胞数、静脈壁タン...

<table border="1"><tbody><tr><td> 消耗品と備品 </td></tr><tr><td>イソフルランガス</td></tr><tr><td> Prolene 7から0縫合</td></tr><tr><td> Vicryl 5から0縫合</td></tr><tr><td>皮膚接着剤</td></tr><tr><td>眼潤滑</td></tr><tr><td> Nolvasanソリューションまたは静菌ソリューション</td></tr><tr><td>実体顕微鏡スコープ</td></tr><tr><td> MediCapture </td></tr><tr><td>コットンアプリケーター</td></tr><tr><td> 2 × 2ガーゼ</td></tr><tr><td>暖房パッド</td></tr><tr><td>麻酔器</td></tr><tr><td>ガラスビーズ器具消毒器</td></tr><tr><td>マイクロ手術器具</td></tr><tr><td>腹部Restrainers </td></tr></tbody></table>

mouse complete stasis model of inferior vena cava thrombosis

静脈血栓塞栓症（VTE）は、深部静脈血栓症（DVT）と肺塞栓症（PE）の両方が含まれています。米国（US）、高い罹患率と死亡率に率は深刻な健康上の懸念1-2 VTE行う。心臓病と脳卒中の後に、VTEは番目の最も一般的な血管疾患の3です。単独で米国では、毎年影響を推定90万人は30万の死亡は毎年3を発生すると、そこにあります。この病気のメカニズムを研究するためのin vivo動物モデルでの信頼性が必要です。 下大静脈血栓症のマウス完全なうっ滞モデルを使用する利点はいくつかある。マウスモデルでは、劇的にコストを削減し、限られた可用性のテストエージェントの非常に小さなボリュームの管理が可能になります。最も有望な特定の炎症と凝固因子の機能が線引きされるように遺伝子ノックアウトによるマウスの可能性があります。現在の分子アッセイは、静脈の壁、血栓、全血、及びアッセイのためのプラズマの定量を可能にする。しかし、このモデルを含む主要な関心事は、手術のサイズの制約と血管のもろさです。また、小さなIVCのサンプル重量（0.005グラムを意味する）に起因するそれは、このようなリアルタイムポリメラーゼ連鎖反応（RT - PCR）、ウェスタンブロットなどの動物の組織のための正確な統計分析のための番号、血栓、および血アッセイを大きくする必要がある酵素結合免疫吸着法（ELISA）、ザイモグラフィー、静脈壁および血栓細胞の解析、および全血と血漿アッセイ4-8。 うっ滞モデルの大きな欠点は、血流の不足が血栓と静脈の壁に投与する全身治療薬の最大効果を阻害することです。

Watch this Scientific Journal Video about Mouse Complete Stasis Model of Inferior Vena Cava Thrombosis at JoVE.com

Mouse Complete Stasis Model of Inferior Vena Cava Thrombosis

下大静脈血栓症のマウス完全なうっ滞モデルは、静脈壁の組織と血栓の定量化可能な量が得られます。それは静脈壁と閉塞性血栓の間に、急性から慢性の炎症への進行を評価する上での相互作用を評価するために役立っています。

下大静脈血栓症のマウス完全な停滞のモデル

Venous thromboembolism (VTE) includes both deep vein thrombosis (DVT) and pulmonary embolism (PE).  In the United States (U.S.), the high morbidity and ...

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Research

JoVE Journal

Medicine

18.1K ビュー.  University of Michigan. 下大静脈血栓症のマウス完全なうっ滞モデルは、静脈壁の組織と血栓の定量化可能な量が得られます。それは静脈壁と閉塞性血栓の間に、急性から慢性の炎症への進行を評価する上での相互作用を評価するために役立っています。

ビデオ: Mouse Complete Stasis Model of Inferior Vena Cava Thrombosis

Electrolytic Inferior Vena Cava Model (EIM) of Venous Thrombosis

Animal models serve a vital role in deep venous thrombosis (DVT) research in order to study thrombus formation, thrombus resolution and to test potential therapeutic compounds (1). New compounds to be utilized in the treatment and prevention of DVT are currently being developed. The delivery of potential therapeutic antagonist compounds to an affected thrombosed vein has been problematic. In the context of therapeutic applications, a model that uses partial stasis and consistently generates thrombi within a major vein has been recently established. The Electrolytic Inferior vena cava Model (EIM) is mouse model of DVT that permits thrombus formation in the presence of continuous blood flow. This model allows therapeutic agents to be in contact with the thrombus in a dynamic fashion, and is more sensitive than other models of DVT (1). In addition, this thrombosis model closely simulates clinical situations of thrombus formation and is ideal to study venous endothelial cell activation, leukocyte migration, venous thrombogenesis, and to test therapeutic applications (1). The EIM model is technically simple, easily reproducible, creates consistent thrombi sizes and allows for a large sample (i.e. thrombus and vein wall) which is required for analytical purposes.

Electrolytic Inferior Vena Cava Model EIM of Venous Thrombosis

The electrolytic induction of endothelial activation to the internal surface of the Inferior Vena Cava results in venous type thrombus formation due to endothelial activation and partial blood stasis, two components of Virchow's triad.

静脈血栓症の電解下大静脈モデル（EIM）

Animal models serve a vital role in deep venous thrombosis (DVT) research in order to study thrombus formation, thrombus resolution and to test potential ...

医学

Quantitative Analysis and Characterization of Atherosclerotic Lesions in the Murine Aortic Sinus

Atherosclerosis is a disease of the large arteries and a major underlying cause of myocardial infarction and stroke. Several different mouse models have been developed to facilitate the study of the molecular and cellular pathophysiology of this disease. In this manuscript we describe specific techniques for the quantification and characterization of atherosclerotic lesions in the murine aortic sinus and ascending aorta. The advantage of this procedure is that it provides an accurate measurement of the cross-sectional area and total volume of the lesion, which can be used to compare atherosclerotic progression across different treatment groups. This is possible through the use of the valve leaflets as an anatomical landmark, together with careful adjustment of the sectioning angle. We also describe basic staining methods that can be used to begin to characterize atherosclerotic progression. These can be further modified to investigate antigens of specific interest to the researcher. The described techniques are generally applicable to a wide variety of existing and newly created dietary and genetically-induced models of atherogenesis.

We describe procedures to quantify and characterize atherosclerotic lesions in mouse models using precision sectioning of the aortic sinus and ascending aorta combined with histochemical and immunohistochemical analysis.

マウス大動脈洞におけるアテローム性動脈硬化症の病変の定量分析とキャラクタリゼーション

Atherosclerosis is a disease of the large arteries and a major underlying cause of myocardial infarction and stroke. Several different mouse models have ...

Implantation of Inferior Vena Cava Interposition Graft in Mouse Model

Biodegradable scaffolds seeded with bone marrow mononuclear cells (BMCs) are often used for reconstructive surgery to treat congenital cardiac anomalies. The long-term clinical results showed excellent patency rates, however, with significant incidence of stenosis. To investigate the cellular and molecular mechanisms of vascular neotissue formation and prevent stenosis development in tissue engineered vascular grafts (TEVGs), we developed a mouse model of the graft with approximately 1 mm internal diameter. First, the TEVGs were assembled from biodegradable tubular scaffolds fabricated from a polyglycolic acid nonwoven felt mesh coated with &#949;-caprolactone and L-lactide copolymer. The scaffolds were then placed in a lyophilizer, vacuumed for 24 hr, and stored in a desiccator until cell seeding. Second, bone marrow was collected from donor mice and mononuclear cells were isolated by density gradient centrifugation. Third, approximately one million cells were seeded on a scaffold and incubated O/N. Finally, the seeded scaffolds were then implanted as infrarenal vena cava interposition grafts in C57BL/6 mice. The implanted grafts demonstrated excellent patency (&#62;90%) without evidence of thromboembolic complications or aneurysmal formation. This murine model will aid us in understanding and quantifying the cellular and molecular mechanisms of neotissue formation in the TEVG.

To improve our knowledge of cellular and molecular neotissue formation, a murine model of the TEVG was recently developed. The grafts were implanted as infrarenal vena cava interposition grafts in C57BL/6 mice. This model achieves similar results to those achieved in our clinical investigation, but over a far shortened time-course.

マウスモデルにおける下大静脈介在移植の移植

Biodegradable scaffolds seeded with bone marrow mononuclear cells (BMCs) are often used for reconstructive surgery to treat congenital cardiac anomalies. ...

Orthotopic Injection of Breast Cancer Cells into the Mammary Fat Pad of Mice to Study Tumor Growth.

Breast cancer growth can be studied in mice using a plethora of models. Genetic manipulation of breast cancer cells may provide insights into the functions of proteins involved in oncogenic progression or help to discover new tumor suppressors. In addition, injecting cancer cells into mice with different genotypes might provide a better understanding of the importance of the stromal compartment. Many models may be useful to investigate certain aspects of disease progression but do not recapitulate the entire cancerous process. In contrast, breast cancer cells engraftment to the mammary fat pad of mice better recapitulates the location of the disease and presence of the proper stromal compartment and therefore better mimics human cancerous disease. In this article, we describe how to implant breast cancer cells into mice orthotopically and explain how to collect tissues to analyse the tumor milieu and metastasis to distant organs. Using this model, many aspects (growth, angiogenesis, and metastasis) of cancer can be investigated simply by providing a proper environment for tumor cells to grow.

Cancer is a complex disease that is influenced by the tissue surrounding the tumor as well as local pro- and anti-inflammatory mediators. Therefore, orthotropic injection models, rather than subcutaneous models may be useful to study cancer progression in a manner that better mimics human pathology.

腫瘍増殖を研究するためにマウスの乳房脂肪パッド中に乳癌細胞の同所注入。

Breast cancer growth can be studied in mice using a plethora of models. Genetic manipulation of breast cancer cells may provide insights into the ...

Ferric Chloride-induced Thrombosis Mouse Model on Carotid Artery and Mesentery Vessel

Severe thrombosis and its ischemic consequences such as myocardial infarction, pulmonary embolism and stroke are major worldwide health issues. The ferric chloride injury is now a well-established technique to rapidly and accurately induce the formation of thrombi in exposed veins or artery of small and large diameter. This model has played a key role in the study of the pathophysiology of thrombosis, in the discovery and validation of novel antithrombotic drugs and in the understanding of the mechanism of action of these new agents. Here, the implementation of this technique on a mesenteric vessel and carotid artery in mice is presented. The method describes how to label circulating leukocytes and platelets with a fluorescent dye and to observe, by intravital microscopy on the exposed mesentery, their accumulation at the injured vessel wall which leads to the formation of a thrombus. On the carotid artery, the occlusion caused by the clot formation is measured by monitoring the blood flow with a Doppler probe.

The FeCl3 induced thrombosis model in mice is described herein. A method to monitor thrombus growth by intravital microscopy observation on a mesenteric vessel and by blood flow measurement in the carotid artery is presented.

塩化第二鉄誘発性頚動脈および腸間膜血管に血栓症のマウスモデル

Severe thrombosis and its ischemic consequences such as myocardial infarction, pulmonary embolism and stroke are major worldwide health issues. The ferric ...

Ferric Chloride-induced Murine Thrombosis Models

Arterial thrombosis (blood clot) is a common complication of many systemic diseases associated with chronic inflammation, including atherosclerosis, diabetes, obesity, cancer and chronic autoimmune rheumatologic disorders. Thrombi are the cause of most heart attacks, strokes and extremity loss, making thrombosis an extremely important public health problem. Since these thrombi stem from inappropriate platelet activation and subsequent coagulation, targeting these systems therapeutically has important clinical significance for developing safer treatments. Due to the complexities of the hemostatic system, in vitro experiments cannot replicate the blood-to-vessel wall interactions; therefore, in vivo studies are critical to understand pathological mechanisms of thrombus formation. To this end, various thrombosis models have been developed in mice. Among them, ferric chloride (FeCl3) induced vascular injury is a widely used model of occlusive thrombosis that reports platelet activation and aggregation in the context of an aseptic closed vascular system. This model is based on redox-induced endothelial cell injury, which is simple and sensitive to both anticoagulant and anti-platelets drugs. The time required for the development of a thrombus that occludes blood flow gives a quantitative measure of vascular injury, platelet activation and aggregation that is relevant to thrombotic diseases. We have significantly refined this FeCl3-induced vascular thrombosis model, which makes the data highly reproducible with minimal variation. Here we describe the model and present representative data from several experimental set-ups that demonstrate the utility of this model in thrombosis research.

We report a refined procedure of the ferric chloride (FeCl3)-induced thrombosis models on carotid and mesenteric artery as well as vein, characterized efficiently using intravital microscopy to monitor time to occlusive thrombi formation.

塩化第二鉄誘発性マウス血栓症モデル

Arterial thrombosis (blood clot) is a common complication of many systemic diseases associated with chronic inflammation, including atherosclerosis, ...

Patch Angioplasty in the Rat Aorta or Inferior Vena Cava

Pericardial patches are commonly used in vascular surgery to close vessels. To facilitate studies of the neointimal hyperplasia that forms on the patch, we developed a rat model of patch angioplasty that can be used in either a vein or an artery, creating a patch venoplasty or a patch arterioplasty, respectively. Technical aspects of this model are discussed. The infra-renal IVC or aorta are dissected and then clamped proximally and distally. A 3 mm venotomy or arteriotomy is performed in the infrarenal inferior vena cava or aorta of 6 to 8 week-old Wistar rats. A bovine pericardial patch (3 mm x 1.5 mm x 0.6 mm) is then used to close the site using a 10-0 nylon suture. Compared to arterial patches, venous patches show increased neointimal thickness on postoperative day 7. This novel model of pericardial patch angioplasty can be used to examine neointimal hyperplasia on vascular biomaterials, as well as to compare the differences between the arterial and venous environments.

We have established a model of pericardial patch angioplasty that can be used in either small-diameter veins or arteries. This model can be used to compare venous and arterial neointimal hyperplasia formation.

ラット大動脈または下大静脈内のパッチ血管形成術

Pericardial patches are commonly used in vascular surgery to close vessels. To facilitate studies of the neointimal hyperplasia that forms on the patch, ...

A Rat Model of Orthotopic Liver Transplantation Using a Novel Magnetic Anastomosis Technique for Suprahepatic Vena Cava Reconstruction

The rat model of orthotopic liver transplantation (OLT) is essential for transplant research. It is a very sophisticated animal model and requires a steep learning curve. The introduction of the cuff technique for anastomosis of the portal vein (PV) and infrahepatic vena cava (IHVC) has significantly simplified the transplant procedure in rats. However, due to the short anterior wall of the recipients' suprahepatic vena cava (SHVC), the cuff technique is very difficult to use for the reconstruction of the SHVC. Most researchers in this field still use the hand-suture technique for SHVC reconstruction, which makes it the bottleneck step in rat orthotopic liver transplantation. The magnetic anastomosis technique (i.e., magnamosis) is a method of connecting two vessels using the attractive force between two magnets. Our recent study has shown that the magnetic anastomosis technique is superior to the hand-suture technique for SHVC reconstruction in rats. In this article, we show a step-by-step protocol for SHVC reconstruction in rats using the novel magnetic anastomosis technique. In this model, the reconstruction of the PV and IHVC was performed by the standard cuff technique, while the reconstruction of the bile duct (BD) was performed by a stent technique. The hepatic re-arterialization was not performed. The magnetic anastomosis technique made SHVC reconstruction much easier and significantly shortened the anphepatic phase. After a reasonable learning curve, even researchers without advanced microsurgical skills can produce reliable and reproducible results using this rat model of OLT.

The reconstruction of the suprahepatic vena cava (SHVC) remains a difficult step in rat orthotopic liver transplantation. In this article, we show a step-by-step protocol for SHVC reconstruction in rats using a novel magnetic anastomosis technique.

同所性同種のモデルラット Suprahepatic 上大静脈再建のため新規磁気吻合法を用いた肝移植

The rat model of orthotopic liver transplantation (OLT) is essential for transplant research. It is a very sophisticated animal model and requires a steep ...

Ferric Chloride-induced Canine Carotid Artery Thrombosis: A Large Animal Model of Vascular Injury

Occlusive arterial thrombosis leading to cerebral ischemic stroke and myocardial infarction contributes to ~13 million deaths every year globally. Here, we have translated a vascular injury model from a small animal into a large animal (canine), with slight modifications that can be used for pre-clinical screening of prophylactic and thrombolytic agents. In addition to the surgical methods, the modified protocol describes the step-by-step methods to assess carotid artery canalization by angiography, detailed instructions to process both the brain and carotid artery for histological analysis to verify carotid canalization and cerebral hemorrhage, and specific parameters to complete an assessment of downstream thromboembolic events by utilizing magnetic resonance imaging (MRI). In addition, specific procedural changes from the previously well-established small animal model necessary to translate into a large animal (canine) vascular injury are discussed.

Here, we present the modifications necessary to a well characterized and commonly used small animal ferric chloride-induced (FeCl3) carotid artery injury model for use in a large animal vascular injury model. The resulting model can be utilized for pre-clinical trial assessment of both prophylactic and thrombolytic pharmacological and mechanical interventions.

塩化第二鉄によるイヌ総頚動脈血栓症: 血管損傷の大動物モデル

Occlusive arterial thrombosis leading to cerebral ischemic stroke and myocardial infarction contributes to ~13 million deaths every year globally. Here, ...

Isometric Contractility Measurement of the Mouse Mesenteric Artery Using Wire Myography

The wire myograph technique is used to assess the contractility of vascular smooth muscles in response to depolarization, GPCR agonists/inhibitors and drugs. It is widely used in many studies on the physiological functions of vascular smooth muscle, the pathogenesis of vascular diseases such as hypertension, and the development of smooth muscle relaxant drugs. The mouse is a widely used model animal with a large pool of disease models and genetically modified strains. We introduced this method to measure the isometric contraction of mouse mesenteric artery in detail. A 1.4-mm segment of mouse mesenteric resistance artery was isolated and mounted on a myograph chamber by passing two steel wires through its lumen. After equilibration and normalization steps, the vessel segment was potentiated by a high-K+ solution twice prior to the contraction assay. As an example of the application of this method in drug development, we measured the relaxant effect of a novel natural substance, neoliensinine, isolated from a Chinese herb, embryos of the lotus seed (Nelumbo nucifera Gaertn.) on mouse mesenteric arteries. The vessel segments mounted on the myograph chamber were stimulated with a high-K+ solution. When the force tension reached a stable sustained phase, cumulative doses of neoliensinine were added to the chamber. We found that neoliensinine had a dose-dependent relaxant effect on smooth muscle contraction, thus suggesting that it bears potential activity against hypertension. In addition, as the vessel segment can survive at least 4 hours after mounting and maintain contractility induced by the high-K+ solution for many times, we suggest that the wire myograph system may be used for the time-consuming process of drug screening.

The wire myograph technique is used to investigate vascular smooth muscle functions and screen new drugs. We report a detailed protocol for measuring the isometric contractility of the mouse mesenteric artery and for screening new relaxants of vascular smooth muscle.

ワイヤー図法を使用してマウスの腸間膜動脈の等尺性収縮力測定

The wire myograph technique is used to assess the contractility of vascular smooth muscles in response to depolarization, GPCR agonists/inhibitors and ...