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AFM-based Mapping of the Elastic Properties of Cell Walls: at Tissue, Cellular, and Subcellular Resolutions

12.8K Views

10:26 min

July 24th, 2014

July 24th, 2014

12,765 Views

0:05

Title

1:17

Preparing Glass Slides for Mounting Samples

2:06

Dissecting and Mounting Samples

4:54

AFM Preparation and Sensitivity Calibration

6:44

Data Acquisition: Apparent Young's Modulus Cartography

7:49

Data Analysis: Apparent Young's Modulus Calculations

8:42

Results: Mechanical Properties of Plant Tissues Measured by AFM Micro/Nano-indentations

10:06

Conclusion

We describe a method to map mechanical properties of plant tissues using an atomic force microscope (AFM). We focus on how to record mechanical changes that take place in cell walls during plant development at wide-field mesoscale, enabling these changes to be correlated with growth and morphogenesis.

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