Name: acquisition of high-quality digital video of drosophila larval and adult behaviors from a lateral perspective
Uploaded: 2014-10-04T15:00:00.000+00:00
Duration: 7 min
Description: Watch this Scientific Journal Video about Acquisition of High-Quality Digital Video of Drosophila Larval and Adult Behaviors from a Lateral Perspective at JoVE.com

著者らは、ビデオのナレーションを提供するための技術支援やサポート、ジェームズ·バートンのためのアレクサンドラオピーを承認したい、付属のビデオに登場するためのラモナFlatzとJoellenスウィーニー。この作品は、MJマードック公益信託（JEDに認可番号2012205）によってサポートされていました。

1ステレオ顕微鏡システム注：このプロトコルは、簡単にビデオを取得する機能を備えたデジタルカメラに接続された事実上すべての実体顕微鏡システムに適用可能であるが、詳細は（材料/機器の表）の研究室で使用されるシステム上に設けられている。 <ol><li>商業デジタルカメラに接続された三眼実体顕微鏡を使用してデジタルビデオを取得します。 </li><li>結合するために実体顕微鏡の三眼ポートへの商用デジタルカメラ、実体顕微鏡の光電管ポートの½xのCマウントを取り外し、1X Cマウントと交換します。 </li><li> 1X Cマウントデジタルカメラカプラー（43ミリメートルスレッド）をマウントします。 </li><li>カメラカプラーにマウント2のステップダウンリング、48ミリメートル、43ミリメートルと48ミリメートル58ミリメートル、デジタルカメラ用レンズアダプターキットデジタルカメラカプラからの接続をブリッジする。 </li><li>レンズアダプターキットにデジタルカメラをマウントします。 </li><li>顕微鏡の倍率は約12倍（毎秒30フレーム、640×480ピクセル）を合わせた倍率デジタルカメラセットの光学ズームでビデオを取得します。注：実体顕微鏡の倍率は三眼港の新たに再構成1X Cマウントに合わせて補償しなければならない。 </li></ol> 2イメージングショウジョウバエ三齢幼虫<ol><li>マーカーキャップの側面には、カメラの液晶モニターで観察された垂直視野の4分の1に⅓約占めるようにデジタルカメラに接続された実体顕微鏡の黒ステージプレートにテープ油性マジックを。それらはカラーコードを使用して画像化されている幼虫の遺伝子型を区別することができる色の品揃えに来るので、幼虫の撮影を行うためにステージとして、マーカートップを使用してください。 </li><li>細かい点でのマーカートップの表面にデジタルカメラの液晶モニターで観察視野を画定マーカー。 </li><li>画像への3齢幼虫を選択します。 3齢幼虫を選択するための基準は、体長、ライフサイクルの幼虫期の間の食物源から出現、前方および後方気門の存在、と口装置11の下顎のフックの構造だった。幼虫は水中で徹底的に洗浄することにより、クリーンであることを確認してください。 </li><li>光ファイバー照明システムからの光を上方から永久マーカー上部ステージを照らす。最適な照明を提供するために入射光の角度を調整する。 </li><li>油性ペン上部の端に顕微鏡を焦点。デジタルビデオを取得開始します。 </li><li>視野（ 図1）の方を向いている幼虫の前で、ちょうど視野外、約75°離れて垂直軸からのマーカーのキャップの側面に幼虫を置きます。注：マーカーキャップ側の幼虫の配置は、番目の記録的動きにカメラを可能にする横方向の視点からの電子幼虫。それは彼らが、マーカーキャップの側面から落下しないように、水で湿った幼虫を維持するのに役立ちます。ケアは、それが視野にわたってクロールのように過剰な量を幼虫に付着するようにあまりにも多くの水を使用しないように、しかし、注意しなければならない。 </li><li>そっと突くと、視野全体にクロールすることを強制するために、小さな絵筆で幼虫をprod。幼虫はほとんど協力しておらず、多くの場合、彼らはまっすぐに、フィールド全体にクロールする前に、開始点に何度も返されなければならないように我慢して。 </li><li>録画中断のデジタルビデオ映像と作物の約10〜15分であり、デジタルビデオ編集ソフトウェアを使用して、すべての不要な映像の取得後を削除します。 </li></ol> 3イメージング大人ショウジョウバエ <ol><li>使い捨て1.5ミリリットル分光ポリスチレンキュベット中で、単一の大人のショウジョウバエを置きます。 注：大人のショウジョウバエのCO 2 anaesthetization直ちにbehav前にioral分析プロトコルは、結果が12を危うくする可能性がある。大人のショウジョウバエは、行動試験13で実行する前に、CO 2 anaesthetizationから回復するために、24時間の期間が与えられることをお勧めします。 </li><li>小さなコットンボールとキュベットの端を差し込みます。コットンボールは、大きなキャップスペースを占有するのに十分タイトなパックされていることを確認し、キュベットの減少容積区画にフライを閉じ込める。 </li><li>実体顕微鏡の白い舞台板にキュベットを置き、適切にデジタルカメラの液晶モニターで観察視野を持つキュベットの減少容積区画を合わせます。 </li><li>光ファイバー照明システムからの光を上方からキュベットを照射する。最適な照明を提供するために入射光の角度を調整する。 </li><li>顕微鏡の焦点を、デジタルビデオの収集を開始。 </li><li>録画中断のデジタルビデオ映像と作物の約30〜45分であり、すべての不要なを削除デジタルビデオ編集ソフトウェアを使用してフッテージ取得後。 </li></ol>

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著者らは競合する利益が存在しないことを宣言した。

神経系の機能を研究するためのモデル系としてキイロショウジョウバエの強みは主に利用可能な強力な遺伝子ツールの収束と開発された堅牢な行動アッセイ幅広い由来する。ここでは、 ショウジョウバエの成体および横方向の視点から幼虫機関車の表現型の高品質のデジタルビデオを取得するためのシンプルな、そして幅広いアクセスができる顕微鏡技術を提示する。私たちは...

一般的な果物飛ぶキイロショウジョウバエは、神経系1-3の機能を研究するための強力な実験モデル系である。進化人間と神経系の構造と機能の保全だけでなく、遺伝子操作の容易性と遺伝子ツールの広大な配列は、ヒトの神経変性疾患4をモデル化するために、ショウジョウバエの初演生物を作る。神経系の機能障害を引き起こす遺伝子突然変異は、多くの場合、障害のある歩行を実現可能変異体幼虫と大人のショウジョウバエにつながる。神経系欠損変異株で観察された表現型は、歩行、異常な調整、および成人における痙性運動の速度だけでなく、体壁筋系の蠕動収縮の欠損、および幼虫の部分的な麻痺の減少、。これらの表現型は、ハイスループット遺伝子スクリーニングおよび突然変異体の幼虫5の移動アッセイの開発に利用されている、運動機能障害を定量化し、神経系の機能に必要な遺伝子を同定することを目的と6および成人7-10 ショウジョウバエ 。これらのアプローチは、幼虫と大人の機関車の挙動を定量化するために極めて有用であるが、これらはそれぞれ、特定の異常な行動についての定性的な情報を伝えることができない。変異型3齢幼虫が行動アッセイで変更された歩行パラメータを示すことができるしながら、これはクロール·サイクル中にリズミカルな蠕動性収縮、協調の一般的な不足、または後部胴体の部分的な麻痺の変化の結果である場合、それは不明かもしれ壁の筋肉組織。ここでは、 ショウジョウバエの成体および横方向の視点から幼虫機関車の表現型の高品質のデジタルビデオを取得するためのシンプルな、そして幅広いアクセスができる顕微鏡技術が記載されている。横視点から取得されたデジタルビデオは、locomotivの微妙な違いを直接観察や分析を可能にするより有益なサイドビューの方向から電子の振る舞い​​。

<table><tbody><tr><td>Trinocular Stereozoom Microscope</td><td>Olympus Corporation</td><td>SZ6145TR</td><td>&amp;frac12; C-mount was removed and replaced with 1X C-mount</td></tr><tr><td>1X C-mount</td><td>Leeds Precision Instruments</td><td>LSZ-1XCMT2</td><td></td></tr><tr><td>Digital Camera Coupler (43 mm thread)</td><td>Qioptiq Imaging Solutions</td><td>25-70-10-02</td><td></td></tr><tr><td>58 mm to 48 mm Step Down Ring</td><td>B&amp;amp;H Video</td><td>GBSDR5848</td><td></td></tr><tr><td>48 mm to 43 mm Step Down Ring</td><td>B&amp;amp;H Video</td><td>GBSDR4843</td><td></td></tr><tr><td>Lensmate Adapter Kit for Canon G10</td><td>LensMateOnline.com</td><td></td><td></td></tr><tr><td>Canon PowerShot G10 Digital Camera</td><td>Canon U.S.A., Inc.</td><td></td><td></td></tr><tr><td>1.5 ml Spectroscopic Polysterene Cuvette</td><td>Denville Scientific</td><td>U8650-4</td><td></td></tr></tbody></table>

acquisition of high-quality digital video of drosophila larval and adult behaviors from a lateral perspective

キイロショウジョウバエは、神経系の機能を研究するための強力な実験モデル系である。神経系の機能障害を引き起こす遺伝子突然変異は、多くの場合、実行可能な幼虫と適切にテキストを記述するか、完全に単一の写真画像では表現が困難運動の欠陥表現型を持っている大人を作り出す。科学的な出版の現在のモードが、しかし、原稿を伴うために補足資料などのデジタル映像メディアの提出をサポートしています。ここでは、横方向の両方の観点から、ショウジョウバエの幼虫と大人の表現型の高品質のデジタルビデオを取得するためのシンプルな、そして幅広いアクセスができる顕微鏡技術が記載されている。それは異常な機関車の挙動に微妙な違いや変化の観察と分析を可能にするため、サイドビューから幼虫と大人の運動のビデオが有利である。私たちは正常に視覚化し、aberran定量化するために技術を使用していたtはグルーミングを含む成人の変異体の表現型と行動に加えて、3齢幼虫に行動をクロール。

私たちは、 スタスミン遺伝子 （ 図2）14の機能の喪失に関連した幼虫の行動表現型を獲得し、定量化するためにこの技術を正常に使用しています。 スタスミン遺伝子は、可溶性チューブリンのプールからチューブリン二量体を分割し、微小管を結合し、その分解を促進する微小管15,16調節タンパク質をコードしている。スタスミン機能が末?...

Watch this Scientific Journal Video about Acquisition of High-Quality Digital Video of Drosophila Larval and Adult Behaviors from a Lateral Perspective at JoVE.com

Acquisition of High-Quality Digital Video of Drosophila Larval and Adult Behaviors from a Lateral Perspective

ここでは、 ショウジョウバエの成体および横方向の視点から幼虫の変異体の表現型の高品質のデジタルビデオを取得するためのシンプルで広くアクセス顕微鏡技術が記載されている。

の高品質のデジタルビデオを買収<em&gt;ショウジョウバエ</em横から見た&gt;幼虫と大人の行動

Drosophila melanogaster is a powerful experimental model system for studying the function of the nervous system. Gene mutations that cause dysfunction of ...

acquisition-high-quality-digital-video-drosophila-larval-adult

Research

JoVE Journal

Neuroscience

Acquisition of High-Quality Digital Video of Drosophila Larval and Adult Behaviors from a Lateral Perspective

8.8K ビュー.  Willamette University. ここでは、 ショウジョウバエの成体および横方向の視点から幼虫の変異体の表現型の高品質のデジタルビデオを取得するためのシンプルで広くアクセス顕微鏡技術が記載されている。 

ビデオ: Acquisition of High-Quality Digital Video of Drosophila Larval and Adult Behaviors from a Lateral Perspective

Live-imaging of the Drosophila Pupal Eye

Inherent processes of Drosophila pupal development can shift and distort the eye epithelium in ways that make individual cell behavior difficult to track during live cell imaging. These processes include: retinal rotation, cell growth and organismal movement. Additionally, irregularities in the topology of the epithelium, including subtle bumps and folds often introduced as the pupa is prepared for imaging, make it challenging to acquire in-focus images of more than a few ommatidia in a single focal plane. The workflow outlined here remedies these issues, allowing easy analysis of cellular processes during Drosophila pupal eye development. Appropriately-staged pupae are arranged in an imaging rig that can be easily assembled in most laboratories. Ubiquitin-DE-Cadherin:GFP and GMR-GAL4&#8211;driven UAS-&#945;-catenin:GFP are used to visualize cell boundaries in the eye epithelium 1-3. After deconvolution is applied to fluorescent images captured at multiple focal planes, maximum projection images are generated for each time point and enhanced using image editing software. Alignment algorithms are used to quickly stabilize superfluous motion, making individual cell behavior easier to track.

Live-imaging of the Drosophila Pupal Eye

This protocol presents an efficient method for imaging the live Drosophila pupal eye neuroepithelium. This method compensates for tissue movement and uneven topology, enhances visualization of cell boundaries through the use of multiple GFP-tagged junction proteins, and uses an easily-assembled imaging rig.

のライブイメージング<em&gt;ショウジョウバエ</em&gt;蛹アイ

Inherent processes of Drosophila pupal development can shift and distort the eye epithelium in ways that make individual cell behavior difficult to track ...

発生生物学

High-Resolution Video Tracking of Locomotion in Adult Drosophila Melanogaster

Flies provide an important model for studying complex behavior due to the plethora of genetic tools available to researchers in this field. Studying locomotor behavior in Drosophila melanogaster relies on the ability to be able to quantify changes in motion during or in response to a given task. For this reason, a high-resolution video tracking system, such as the one we describe in this paper, is a valuable tool for measuring locomotion in real-time. Our protocol involves the use of an initial air pulse to break the flies momentum, followed by a thirty second filming period in a square chamber. A tracking program is then used to calculate the instantaneous speed of each fly within the chamber in 10 msec increments. Analysis software then compiles this data, and outputs a variety of parameters such as average speed, max speed, time spent in motion, acceleration, etc. This protocol will discuss proper feeding and management of flies for behavioral tasks, handling flies without anesthetization or immobilization, setting up a controlled environment, and running the assay from start to finish.

The study of complex locomotor behavior in Drosophila melanogaster is dependent upon the ability to quantify changes in a given fly's movement. This article demonstrates how to do this using a high-resolution tracking system.

アダルトキイロショウジョウバエのロコモーションの高解像度のビデオトラッキング

Flies provide an important model for studying complex behavior due to the plethora of genetic tools available to researchers in this field.  Studying ...

生物学

An Adjustable High-Definition Imaging System for Behavioral Studies of Drosophila Adults

Drosophila melanogaster is a very powerful model in biological research, but a bad model for photography or videography. This paper describes a simple but effective method to observe and document the behavior or morphology of flies. Flies were placed in a translucent observation chamber c.a. &#216;15 x 5mm (no food inside) or &#216;15 x 12 mm (with an 8 mm-high piece of food inside). After covering with an ultraviolet (UV)/clear filter with high light transmittance, the chamber was placed under a 5-50x zoom stereo microscope, and mini light-emitting diode (LED) video lights were placed on both sides of the microscope to illuminate the chamber to obtain uniform, soft, bright, and nearly shadow-free light. Then, a compact digital camera with 3-5x optical zoom, which can record 1080 P high-definition or higher resolution video (at a frame rate of &#8805;30 fps), was connected to the eyepiece of microscope through a bracket, and photographs or videos were taken through the eyepiece. By adjusting the zoom knob of the zoom stereo microscope, it was very easy to track the flies and take panoramic or detailed close-up images as needed, while the camera recorded everything under the microscope. Because the flies can stay at any position in the chamber, they can be observed and recorded from all directions. The photographs or videos taken are of good image quality. This method can be used both for scientific research and teaching.

An Adjustable High-Definition Imaging System for Behavioral Studies of Drosophila Adults

This protocol describes how to make a simple adult Drosophila behavior observation chamber, and how to take high-definition photographs/videos of the morphology or behavior of different types of fruit flies in the observation chamber through relatively simple and affordable methods.

ショウジョウバエ成人行動研究のための調整可能な高精細画像化システム

Drosophila melanogaster is a very powerful model in biological research, but a bad model for photography or videography. This paper describes a simple but ...

行動学

Imaging and Analysis of Tissue Orientation and Growth Dynamics in the Developing Drosophila Epithelia During Pupal Stages

Within multicellular organisms, mature tissues and organs display high degrees of order in the spatial arrangements of their constituent cells. A remarkable example is given by sensory epithelia, where cells of the same or distinct identities are brought together via cell-cell adhesion showing highly organized planar patterns. Cells align to one another in the same direction and display equivalent polarity over large distances. This organization of the mature epithelia is established over the course of morphogenesis. To understand how the planar arrangement of the mature epithelia is achieved, it is crucial to track cell orientation and growth dynamics with high spatiotemporal fidelity during development in vivo. Robust analytical tools are also essential to identify and characterize local-to-global transitions. The Drosophila pupa is an ideal system to evaluate oriented cell shape changes underlying epithelial morphogenesis. The pupal developing epithelium constitutes the external surface of the immobile body, allowing long-term imaging of intact animals. The protocol described here is designed to image and analyze cell behaviors at both global and local levels in the pupal abdominal epidermis as it grows. The methodology described can be easily adapted to the imaging of cell behaviors at other developmental stages, tissues, subcellular structures, or model organisms.

Imaging and Analysis of Tissue Orientation and Growth Dynamics in the Developing Drosophila Epithelia During Pupal Stages

This protocol is designed for the imaging and analysis of the dynamics of cell orientation and tissue growth in the Drosophila abdominal epithelia as the fruit fly undergoes metamorphosis. The methodology described here can be applied to the study of different developmental stages, tissues, and subcellular structures in Drosophila or other model organisms.

プパル期のショウジョウバエ上皮の組織オリエンテーションと成長ダイナミクスのイメージングと解析

Within multicellular organisms, mature tissues and organs display high degrees of order in the spatial arrangements of their constituent cells. A ...

A Simple Technique to Assay Locomotor Activity in Drosophila

Drosophila melanogaster is an ideal model organism for studying various diseases due to its abundance of advanced genetic manipulation techniques and diverse behavioral features. Identifying behavioral deficiency in animal models is a crucial measure of disease severity, for example, in neurodegenerative diseases where patients often experience impairments in motor function. However, with the availability of various systems to track and assess motor deficits in fly models, such as drug-treated or transgenic individuals, an economical and user-friendly system for precise evaluation from multiple angles is still lacking. A method based on the AnimalTracker application programming interface (API) is developed here, which is compatible with the Fiji image processing program, to systematically evaluate the movement activities of both adult and larval individuals from recorded video, thus allowing for the analysis of their tracking behavior. This method requires only a high-definition camera and a computer peripheral hardware integration to record and analyze behavior, making it an affordable and effective approach for screening fly models with transgenic or environmental behavioral deficiencies. Examples of behavioral tests using pharmacologically treated flies are given to show how the techniques can detect behavioral changes in both adult flies and larvae in a highly repeatable manner.

A Simple Technique to Assay Locomotor Activity in Drosophila

The present protocol assesses the locomotor activity of Drosophila by tracking and analyzing the movement of flies in a hand-made arena using open-source software Fiji, compatible with plugins to segment pixels of each frame based on high-definition video recording to calculate parameters of speed, distance, etc.

ショウジョウバエの自発運動活性をアッセイする簡単な技術

Drosophila melanogaster is an ideal model organism for studying various diseases due to its abundance of advanced genetic manipulation techniques and ...

In vivo Imaging of Intact Drosophila Larvae at Sub-cellular Resolution

Recent improvements in optical imaging, genetically encoded fluorophores and genetic tools allowing efficient establishment of desired transgenic animal lines have enabled biological processes to be studied in the context of a living, and in some instances even behaving, organism. In this protocol we will describe how to anesthetize intact Drosophila larvae, using the volatile anesthetic desflurane, to follow the development and plasticity of synaptic populations at sub-cellular resolution1-3. While other useful methods to anesthetize Drosophila melanogaster larvae have been previously described4,5,6,7,8, the protocol presented herein demonstrates significant improvements due to the following combined key features: (1) A very high degree of anesthetization; even the heart beat is arrested allowing for lateral resolution of up to 150 nm1, (2) a high survival rate of &gt; 90% per anesthetization cycle, permitting the recording of more than five time-points over a period of hours to days2 and (3) a high sensitivity enabling us in 2 instances to study the dynamics of proteins expressed at physiological levels. In detail, we were able to visualize the postsynaptic glutamate receptor subunit GluR-IIA expressed via the endogenous promoter1 in stable transgenic lines and the exon trap line FasII-GFP1. (4) In contrast to other methods4,7 the larvae can be imaged not only alive, but also intact (i.e. non-dissected) allowing observation to occur over a number of days1. The accompanying video details the function of individual parts of the in vivo imaging chamber2,3, the correct mounting of the larvae, the anesthetization procedure, how to re-identify specific positions within a larva and the safe removal of the larvae from the imaging chamber.

In vivo Imaging of Intact Drosophila Larvae at Sub-cellular Resolution

This protocol describes a reliable method for anesthetization and imaging of intact Drosophila melanogaster larvae. We have utilized the volatile anesthetic desflurane to allow for repetitive imaging at sub-cellular resolution and re-identification of structures for up to a few days1.

サブセルラー解像度で手つかずのショウジョウバエの幼虫の in vivoイメージングで

Recent improvements in optical imaging, genetically encoded fluorophores and genetic tools allowing efficient establishment of desired transgenic animal ...

<meta charset="utf8"></head><body>蛹を伴わない ショウジョウバエ の蛹脚形態形成の拡張ライブイメージング

Long-Term Live Imaging of Drosophila Pupal Leg Development After Puparium Removal

Over the past decades, significant progress has been made in understanding the mechanisms of cell fate determination. However, the process by which fate-determined cells form three-dimensional organismal shapes remains unclear. Recent advances in confocal microscopy have facilitated efforts to observe cell dynamics during development through live imaging. The Drosophila melanogaster pupa is ideal for live imaging due to its immobility, transparent pupal cuticle, and the availability of fluorescent reporter lines. A primary challenge for imaging is the puparium, the cuticle surrounding the pupa, which obstructs optical imaging. While previous methods involved either partial or complete removal of the puparium, maintaining pupal viability for extended periods after this procedure has remained challenging. Here, a simple method is presented for days-long live imaging of the Drosophila leg during the pupal stage, involving complete puparium removal. The method includes removing the puparium from a pupa adhered to double-sided tape, followed by assembling a small chamber on a glass-bottom dish to enclose the pupa and a drop of water. This setup is straightforward, reliable, and supports extended pupal survival by preventing desiccation. Long-term live imaging of the Drosophila pupa has significantly contributed to capturing how the adult leg undergoes dramatic three-dimensional structural changes over 2-3 days. These changes include the transient formation of an intriguing structure (the Parthenon-like structure) by epithelial cells, rapid tissue narrowing, joint formation, and bristle elongation. This method is applicable to the observation of various tissues and can potentially be combined with other techniques, such as optical gene induction, to advance the understanding of cell dynamics during the final shape formation of tissues in the pupal stage.

<meta charset="utf8"></head><body>著者スポットライト: ショウジョウバエ の発生における動的上皮構造の解明

Here, a protocol is presented for long-term live imaging of the Drosophila leg during the pupal stage, involving complete removal of the puparium. This protocol can also be applied to other tissues.

蛹摘出後の ショウジョウバエ の蛹の脚の発達の長期ライブイメージング

Over the past decades, significant progress has been made in understanding the mechanisms of cell fate determination. However, the process by which ...

Time-lapse Live Imaging and Quantification of Fast Dendritic Branch Dynamics in Developing Drosophila Neurons

Highly motile dendritic filopodia are widely present in neurons at early developmental stages. These exploratory dynamic branches sample the surrounding environment and initiate contacts with potential synaptic partners. Although the connection between dendritic branch dynamics and synaptogenesis is well established, how developmental and activity-dependent processes regulate dendritic branch dynamics is not well understood. This is partly due to the technical difficulties associated with the live imaging and quantitative analyses of these fine structures using an in vivo system. We established a method to study dendrite dynamics using Drosophila larval ventral lateral neurons (LNvs), which can be individually labeled using genetic approaches and are accessible for live imaging. Taking advantage of this system, we developed protocols to capture branch dynamics of the whole dendritic arbor of a single labeled LNv through time-lapse live imaging. We then performed post-processing to improve image quality through drift correction and deconvolution, followed by analyzing branch dynamics at the single-branch level by annotating spatial positions of all branch terminals. Lastly, we developed R scripts (Supplementary File) and specific parameters to quantify branch dynamics using the coordinate information generated by the terminal tracing. Collectively, this protocol allows us to achieve a detailed quantitative description of branch dynamics of the neuronal dendritic arbor with high temporal and spatial resolution. The methods we developed are generally applicable to sparsely labeled neurons in both in vitro and in vivo conditions.

Time-lapse Live Imaging and Quantification of Fast Dendritic Branch Dynamics in Developing Drosophila Neurons

Here, we describe the method we employed to image highly motile dendritic filopodia in a live preparation of the Drosophila larval brain, and the protocol we developed to quantify time-lapse 3D imaging datasets for quantitative assessments of dendrite dynamics in developing neurons.

ショウジョウバエニューロンの開発における高速樹状分岐ダイナミクスのタイムラプスライブイメージングと定量化

Highly motile dendritic filopodia are widely present in neurons at early developmental stages. These exploratory dynamic branches sample the surrounding ...

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のライブイメージング

アダルトキイロショウジョウバエのロコモーションの高解像度のビデオトラッキング

ショウジョウバエ成人行動研究のための調整可能な高精細画像化システム

プパル期のショウジョウバエ上皮の組織オリエンテーションと成長ダイナミクスのイメージングと解析

ショウジョウバエの自発運動活性をアッセイする簡単な技術

サブセルラー解像度で手つかずのショウジョウバエの幼虫の in vivoイメージングで

蛹摘出後のショウジョウバエの蛹の脚の発達の長期ライブイメージング

蛹摘出後のショウジョウバエの蛹の脚の発達の長期ライブイメージング

蛹摘出後のショウジョウバエの蛹の脚の発達の長期ライブイメージング

ショウジョウバエニューロンの開発における高速樹状分岐ダイナミクスのタイムラプスライブイメージングと定量化

の高品質のデジタルビデオを買収

要約

さらに動画を探す

この動画の章

のライブイメージング

アダルトキイロショウジョウバエのロコモーションの高解像度のビデオトラッキング

ショウジョウバエ成人行動研究のための調整可能な高精細画像化システム

プパル期のショウジョウバエ上皮の組織オリエンテーションと成長ダイナミクスのイメージングと解析

ショウジョウバエの自発運動活性をアッセイする簡単な技術

サブセルラー解像度で手つかずのショウジョウバエの幼虫の in vivoイメージングで

蛹摘出後の ショウジョウバエ の蛹の脚の発達の長期ライブイメージング

蛹摘出後の ショウジョウバエ の蛹の脚の発達の長期ライブイメージング

蛹摘出後の ショウジョウバエ の蛹の脚の発達の長期ライブイメージング

ショウジョウバエニューロンの開発における高速樹状分岐ダイナミクスのタイムラプスライブイメージングと定量化

蛹摘出後のショウジョウバエの蛹の脚の発達の長期ライブイメージング

蛹摘出後のショウジョウバエの蛹の脚の発達の長期ライブイメージング

蛹摘出後のショウジョウバエの蛹の脚の発達の長期ライブイメージング