JoVE Journal

Genetics

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Primordial Germ Cell Transplantation for CRISPR/Cas9-based Leapfrogging in Xenopus

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05:34 min

February 1st, 2018

February 1st, 2018

8,269 Views

0:04

Title

0:30

Transplantation of PGCs

3:36

Post-healing Care of Embryos

4:39

Results: CRISPR/Cas9 Leapfrogging in Xenopus

5:16

Conclusion

文字起こし

The overall goal of leapfrogging is to facilitate the creation of mutant lines for genes that are essential for embryogenesis. This method can help answer key questions in Developmental Genetics and other fields. The main advantage of this techniq

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Genes essential for survival pose technical hurdles for creating mutant lines. Leapfrogging circumvents lethality by combining genome editing with primordial germ cell transplantation to create wild-type animals carrying germline mutations. Leapfrogging also permits the efficient generation of homozygous null mutants in the F1 generation. Here, the transplantation step is demonstrated.

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