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Human Peripheral Blood Neutrophil Isolation for Interrogating the Parkinson's Associated LRRK2 Kinase Pathway by Assessing Rab10 Phosphorylation

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12:49 min

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March 21st, 2020

DOI :

10.3791/58956-v

March 21st, 2020

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7,250 Views

Ying Fan*¹, Francesca Tonelli*¹, Shalini Padmanabhan², Marco A.S. Baptista², Lindsey Riley², Danielle Smith³, Connie Marras⁴, Andrew Howden⁵, Dario R. Alessi¹, Esther Sammler¹^,⁶

¹MRC Protein Phosphorylation and Ubiquitylation Unit, School of Life Sciences, University of Dundee, ²The Michael J. Fox Foundation for Parkinson's Research, ³Department of Medical and Molecular Genetics, Indiana University School of Medicine, ⁴Morton and Gloria Shulman Movement Disorders Centre and the Edmond J. Safra Program in Parkinson's Disease, Toronto Western Hospital, University of Toronto, ⁵Division of Cell Signalling and Immunology, School of Life Sciences, University of Dundee, ⁶Division of Molecular and Clinical Medicine, School of Medicine, Ninewells Hospital and Medical School, University of Dundee

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Neutrophil Isolation

LRRK2 Kinase

Rab10 Phosphorylation

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