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Assessing Protein Interactions in Live-Cells with FRET-Sensitized Emission

2.9K Views

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09:15 min

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April 22nd, 2021

DOI :

10.3791/62241-v

April 22nd, 2021

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2,907 Views

György Vámosi¹, Sarah Miller², Molika Sinha², Maria Kristha Fernandez², Gabor Mocsár¹, Malte Renz²

¹Department of Biophysics and Cell Biology, Faculty of Medicine, University of Debrecen, ²Gynecologic Oncology Division, Stanford University School of Medicine

章

文字起こし

要約

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FRET

FRET sensitized Emission

Protein Interactions

Live cell Imaging

Donor Quenching

Acceptor Sensitized Emission

Crosstalk

Fluorescent Proteins

Detection Efficiency

Donor acceptor Fusion Protein

EGFP mCherry

Cell Transfection

Confocal Microscopy

Environmental Chamber

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