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Inselspital, Bern University Hospital, University of Bern

8 ARTICLES PUBLISHED IN JoVE

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Medicine

The Rabbit Blood-shunt Model for the Study of Acute and Late Sequelae of Subarachnoid Hemorrhage: Technical Aspects
Lukas Andereggen 3,4,5, Volker Neuschmelting 1,6, Michael von Gunten 7, Hans Rudolf Widmer 5, Jukka Takala 1, Stephan M. Jakob 1, Javier Fandino 1,2, Serge Marbacher 1,2
1Department of Intensive Care Medicine, University and Bern University Hospital (Inselspital), 2Department of Neurosurgery, Kantonsspital Aarau, 3Laboratories for Neuroscience Research in Neurosurgery, Boston Children's Hospital, 4Harvard Medical School, Boston Children's Hospital, 5Department of Neurosurgery, University and Bern University Hospital (Inselspital), 6Department of Neurosurgery, University Hospital Cologne, 7Institute of Pathology, Länggasse Bern

The experimental intracranial pressure-controlled blood shunt subarachnoid hemorrhage (SAH) model in the rabbit combines the standard procedures — subclavian artery cannulation and transcutaneous cisterna magna puncture, which enables close mimicking of human pathophysiological conditions after SAH. We present step-by-step instructions and discuss key surgical points for successful experimental SAH creation.

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Medicine

Discovering Middle Ear Anatomy by Transcanal Endoscopic Ear Surgery: A Dissection Manual
Lukas Anschuetz 1,2, Livio Presutti 2, Daniele Marchioni 3, Marco Bonali 2, Wilhelm Wimmer 1,4, Domenico Villari 2, Marco Caversaccio 1,4
1Department of Otorhinolaryngology, Head and Neck Surgery, Inselspital, Bern University Hospital, University of Bern, 2Department of Otorhinolaryngology, Head & Neck Surgery, University Hospital of Modena, 3Department of Otorhinolaryngology, Head & Neck Surgery, University Hospital of Verona, 4Artificial Hearing Research, Artorg Center for Biomedical Engineering, University of Bern

The aim of this article is to describe the methodology of exclusively endoscopic cadaveric middle ear dissection. Moreover, we aim to provide a comprehensive guide to endoscopic middle ear anatomy.

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Neuroscience

Arterial Pouch Microsurgical Bifurcation Aneurysm Model in the Rabbit
Stefan Wanderer 1,2, Claudia Waltenspuel 2, Basil E. Grüter 1,2, Fabio Strange 1,2, Sivani Sivanrupan 2, Luca Remonda 3, Hans Rudolf Widmer 4, Daniela Casoni 5, Lukas Andereggen 1,2, Javier Fandino 1,2, Serge Marbacher 1,2
1Department of Neurosurgery, Kantonsspital Aarau, 2Cerebrovascular Research Group, Department for BioMedical Research, University of Bern, 3Division of Neuroradiology, Department of Radiology, Kantonsspital Aarau, 4Department of Neurosurgery, Neurocenter and Regenerative Neuroscience Cluster, Inselspital, Bern University Hospital, University of Bern, 5Department for Biomedical Research, Faculty of Medicine, University of Bern

Developing and testing endovascular devices for intracranial aneurysm treatment is still of great importance. Most aneurysm models used today miss either the important characteristics of an arterial degenerated wall or the hemodynamics of a true bifurcation. Therefore, we aimed to design a novel arterial pouch bifurcation model in rabbits.

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Medicine

The Microscopic Transcanal Approach in Stapes Surgery Revisited
Georgios Mantokoudis 1, Stefan Weder 1, Lukas Anschuetz 1, Sandra Gunser 1, Urs Borner 1, Marco D. Caversaccio 1
1Department of Otorhinolaryngology, Head and Neck Surgery, lnselspital, Bern University Hospital, University of Bern

This article describes the microscopic transcanal technique for stapes surgery, providing step-by-step surgical instructions for familiarizing surgeons with this approach.

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Medicine

Performing Intracochlear Electrocochleography During Cochlear Implantation
Klaus Schuerch 1,2, Manuel Waser 1, Georgios Mantokoudis 1, Lukas Anschuetz 1, Wilhelm Wimmer 1,2, Marco Caversaccio 1,2, Stefan Weder 1
1Department of Otorhinolaryngology, Head and Neck Surgery, Inselspital, Bern University Hospital, University of Bern, 2Hearing Research Laboratory, ARTORG Center for Biomedical Engineering Research, University of Bern

Electrocochleography (ECochG) measures inner ear potentials generated in response to acoustic stimulation. In cochlear implant (CI) candidates, such inner ear potentials can be measured directly with the implant electrodes. In this video, we systematically explain how to perform ECochG recordings during CI surgery.

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Medicine

Endoscopic Cholesteatoma Surgery
Sven Beckmann 1, Georgios Mantokoudis 1, Stefan Weder 1, Urs Borner 1, Marco Caversaccio 1, Lukas Anschuetz 1
1Department of Otorhinolaryngology, Head and Neck Surgery, Inselspital, Bern University Hospital, University of Bern

The present protocol describes a step-by-step guide for the complete endoscopic removal of epitympanic cholesteatoma with different techniques for cholesteatoma dissection and bone removal for epitympanectomy.

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Neuroscience

Using a Cell-Tracer Injection to Investigate the Origin of Neointima-Forming Cells in a Rat Saccular Side Wall Model
Stefan Wanderer *1,2, Basil E. Grüter *1,2, Jeannine Kümin 1,2, Gwendoline Boillat 1,2, Sivani Sivanrupan 2, Kristina Catalano 1,2, Michael von Gunten 3, Hans Rudolf Widmer 4, Serge Marbacher 1,2,5, Lukas Andereggen 1,2,5
1Department of Neurosurgery, Kantonsspital Aarau, 2Cerebrovascular Research Group, Department for BioMedical Research, University of Bern, 3Institute of Pathology Laenggasse, 4Department of Neurosurgery, Neurocenter and Regenerative Neuroscience Cluster, Inselspital, Bern University Hospital, University of Bern, 5Faculty of Medicine, University of Bern

We performed a one-point, lipophilic cell-tracer injection to track endothelial cells, followed by an arteriotomy and suturing of sidewall aneurysms on the abdominal rat aorta. Neointima formation seemed dependent on the parent artery in decellularized aneurysms and was promoted by the recruitment from aneurysm wall cells in vital cell-rich walls.

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Medicine

Robotic Cochlear Implantation for Direct Cochlear Access
Marco Caversaccio 1,2, Georgios Mantokoudis 1, Franca Wagner 3, Philipp Aebischer 1,2, Stefan Weder 1,2, Wilhelm Wimmer 1,2
1Department of Otorhinolaryngology, Head and Neck Surgery, Inselspital, Bern University Hospital, University of Bern, 2Hearing Research Laboratory, ARTORG Center for Biomedical Engineering Research, University of Bern, 3Department of Diagnostic and Interventional Neuroradiology, Inselspital, Bern University Hospital

Robotic cochlear implantation is a procedure for minimally invasive inner ear access. Compared to conventional surgery, robotic cochlear implantation involves additional steps that need to be carried out in the operating room. In this article we give a description of the procedure and highlight the important aspects of robotic cochlear implantation.

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