Stress Treatment of E. coli Strains MG1655 and CFT073
2:12
Collecting Bacterial Cell Samples
2:43
Extracting the Insoluble Protein Aggregates
4:19
Soluble Protein Sample Preparation
5:30
Separation and Visualization of Extracted Protein Aggregates Using SDS-PAGE
6:49
Results: Visualization of Protein Aggregates After Treatment of Escherichia coli with a Proteotoxic Stressor
7:32
Conclusion
필기록
Exposure to stress can cause protein aggregation and result in significant alterations in the phenotypic behavior of bacteria. The procedure described in this video allows the extraction and visualization of protein aggregates after stress treatme
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This protocol describes the extraction and visualization of aggregated and soluble proteins from Escherichia coli after treatment with a proteotoxic antimicrobial. Following this procedure allows a qualitative comparison of protein aggregate formation in vivo in different bacterial strains and/or between treatments.