Method validation is a crucial process in analytical chemistry designed to confirm that a given method consistently produces reliable and high-quality results. This process is essential when a method is applied to different sample matrices or when procedural modifications are made, ensuring that the results meet acceptable standards across various applications.
Key parameters for method validation include:
To assess these parameters, it is necessary to differentiate between random and systematic errors, which can arise from both the method and the analyst performing the analysis. The precision of a method, often related to random errors, can be evaluated using the standard deviation of repeated measurements, reflecting consistency under the same conditions.
Systematic errors often influence accuracy or how close results are to the true value. These can be assessed through various means, such as using standard references, blanks, or varying sample sizes. Additionally, independent testing with alternative methods or conducting the same analysis in different laboratories can reveal systematic errors stemming from the method or the analyst, respectively.
Overall, method validation provides confidence that an analytical method will yield dependable results, regardless of changes in sample types or procedural adjustments.
From Chapter 9:
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