July 19th, 2007
•Introducing a gene of interest into a cell is a powerful method for elucidating its function in vivo. This protocol describes an efficient method of transfecting a culture of human neural stem/precursor cells (hNSPCs) using the Nucleofector electroporation apparatus made by Amaxa.
Tags
Related Videos
Counting Human Neural Stem Cells
Passaging Human Neural Stem Cells
Immunocytochemistry: Human Neural Stem Cells
Labeling Stem Cells with Fluorescent Dyes for non-invasive Detection with Optical Imaging
Mouse Epidermal Neural Crest Stem Cell (EPI-NCSC) Cultures
Generation of Induced Pluripotent Stem Cells by Reprogramming Human Fibroblasts with the Stemgent Human TF Lentivirus Set
Xenotransplantation of Human Stem Cells into the Chicken Embryo
Lentiviral-mediated Knockdown During Ex Vivo Erythropoiesis of Human Hematopoietic Stem Cells
Transfecting and Nucleofecting Human Induced Pluripotent Stem Cells
Reprogramming Human Somatic Cells into Induced Pluripotent Stem Cells (iPSCs) Using Retroviral Vector with GFP
Copyright © 2024 MyJoVE Corporation. 판권 소유