로그인

JoVE Journal

Immunology and Infection

JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다.

Antigens Protected Functional Red Blood Cells By The Membrane Grafting Of Compact Hyperbranched Polyglycerols

17.9K Views

11:31 min

January 2nd, 2013

January 2nd, 2013

17,949 Views

0:05

Title

1:28

Succinimidyl Succinate-Hyperbranched Polyglycerol Modification (SS-HPG)

3:30

Whole Blood Collection and Separation of Red Blood Cells (RBC)

4:26

HPG Grafting to RBC

5:28

Characterization of HPG Modified RBC: Complement Mediated Lysis

7:16

Characterization of HPG Modified RBC: The Camouflage of Major and Minor Ag using MTS Cards

8:08

Characterization of HPG Modified RBC: Aqueous Two Phase Partition Measurements

9:14

Characterization of HPG Modified RBC: Flow Cytometry Measurements - Protection of Rhesus-D (RhD) Antigens

10:12

Results: Representative Evaluation of the Camouflage of Surface Antigens and Proteins using Flow Cytometry

10:58

Conclusion

The cell membrane modification of red blood cells (RBCs) with hyperbranched polyglycerol (HPG) is presented. Modified RBCs were characterized by aqueous two phase partitioning, osmotic fragility and complement mediated lysis. The camouflage of surface proteins and antigens was evaluated using the flow cytometry and Micro Typing System (MTS) blood phenotyping cards.

당사 웹 사이트에서는 사용자의 경험을 향상시키기 위해 쿠키를 사용합니다.

당사 웹 사이트를 계속 사용하거나 '계속'을 클릭하는 것은 당사 쿠키 수락에 동의하는 것을 의미합니다.