Antigens Protected Functional Red Blood Cells By The Membrane Grafting Of Compact Hyperbranched Polyglycerols

18.0K Views

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11:31 min

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January 2nd, 2013

DOI :

10.3791/50075-v

January 2nd, 2013

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Rafi Chapanian¹^,², Iren Constantinescu¹^,³, Donald E. Brooks¹^,²^,⁴, Mark D. Scott¹^,²^,³, Jayachandran Kizhakkedathu¹^,²^,⁴

¹Centre for Blood Research, University of British Columbia , ²Department of Pathology and Laboratory Medicine, University of British Columbia , ³Canadian Blood Services, University of British Columbia , ⁴Department of Chemistry, Life Sciences Centre, University of British Columbia

Summary

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Keywords Antigen Protection

Red Blood Cells

Membrane Grafting

Hyperbranched Polyglycerol

Alloantibodies

Universal Red Blood Donor

Transfusion Safety

Chapters in this video

0:05

Title

1:28

Succinimidyl Succinate-Hyperbranched Polyglycerol Modification (SS-HPG)

3:30

Whole Blood Collection and Separation of Red Blood Cells (RBC)

4:26

HPG Grafting to RBC

5:28

Characterization of HPG Modified RBC: Complement Mediated Lysis

7:16

Characterization of HPG Modified RBC: The Camouflage of Major and Minor Ag using MTS Cards

8:08

Characterization of HPG Modified RBC: Aqueous Two Phase Partition Measurements

9:14

Characterization of HPG Modified RBC: Flow Cytometry Measurements - Protection of Rhesus-D (RhD) Antigens

10:12

Results: Representative Evaluation of the Camouflage of Surface Antigens and Proteins using Flow Cytometry

10:58

Conclusion

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