March 5th, 2013
•A rapid and simple way to generate human cell lines with inducible and reversible cDNA overexpression or shRNA-mediated knock-down of the gene of interest. This method enables researchers to reliably and highly reproducibly manipulate cell lines that are difficult to alter by transient transfection methods or conventional knockdown/knockout strategies.
Related Videos
Passaging HuES Human Embryonic Stem Cell-lines with Trypsin.
Generation of Stable Transgenic C. elegans Using Microinjection
The Preparation of Primary Hematopoietic Cell Cultures From Murine Bone Marrow for Electroporation
Staining of Proteins in Gels with Coomassie G-250 without Organic Solvent and Acetic Acid
Generation of Human CD40-activated B cells
RNA Isolation from Embryonic Zebrafish and cDNA Synthesis for Gene Expression Analysis
Using the Gene Pulser MXcell Electroporation System to Transfect Primary Cells with High Efficiency
Using an Automated Cell Counter to Simplify Gene Expression Studies: siRNA Knockdown of IL-4 Dependent Gene Expression in Namalwa Cells
Transduction of Human Cells with Polymer-complexed Ecotropic Lentivirus for Enhanced Biosafety
Preparation of Cell-lines for Conditional Knockdown of Gene Expression and Measurement of the Knockdown Effects on E4orf4-Induced Cell Death
Copyright © 2024 MyJoVE Corporation. 판권 소유