신경 세포 유형의 표면에 대한 유동 세포 계측법 프로토콜 및 세포 내 항원 분석

1:03

Neural Cell Harvesting

3:10

Antibody-independent Intracellular Labeling with Carboxyfluorescein Succinimidyl Ester (CFSE)

4:49

Cell-surface Antigen Staining with Fluorophore-conjugated Antibodies

5:56

Fixation and Membrane Permeabilization

7:35

Intracellular Antigen Staining with Non-fluorescent Primary Antibodies

9:30

Flow Cytometry Analysis

10:37

Results: Multicolor Flow Cytometric Analysis of Surface vs. Introcellular Antigens in Neural Cells

12:36

Conclusion