Measuring siRNA Release Using Gell Electrophoresis
4:57
Measuring siRNA Release Using Fluorescence Correlation Spectroscopy (FCS)
6:12
Cell Culture and In Vitro siRNA Delivery
8:15
Controlling Gene Silencing in a Spatiotemporal Manner
9:35
Results: Prediction and Spatiotemporal Control of Gene Silencing
11:07
Conclusion
필기록
The overall goal of these methods is to unable the prediction of small interfering RNA mediated gene silencing efficiencies and facilitate control over the resulting protein expression levels, in a spatial temporal manner. This method can elucidat
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We present a novel method that uses photo-responsive block copolymers for more efficient spatiotemporal control of gene silencing with no detectable off-target effects. Additionally, changes in gene expression can be predicted using straightforward siRNA release assays and simple kinetic modeling.