Preparation of Late-stage Embryos for Immunostaining
4:19
LacZ Staining and Immunostaining
7:20
Preparing Embryos to Image the ISN Nerves in Abdominal Segments 7 to A9
10:03
Results: Intersegmental Nerve Bundle (ISNb) Innervation at A3 and A4
11:21
Conclusion
필기록
The overall goal of this experiment is to analyze the motor neuron projection patterns in late stage embryos of Drosophila melanogaster. This method can help answer key questions in the field of neural development, such as motor axon guidance and
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This work details a standard immunohistochemistry method to visualize motor neuron projections of late stage-16 Drosophila melanogaster embryos. The filleted preparation of fixed embryos stained with FasII antibody provides a powerful tool to characterize the genes required for motor axon pathfinding and target recognition during neural development.