Harvesting and Embedding the VCCC for Transverse Immunofluorescence
9:11
Results: Myoendothelial Junction Formation in Vascular Cell Co-cultures
10:18
Conclusion
필기록
The overall goal of this technique is to culture endothelial and smooth muscle cells together in-vitro to create myoendothelial junctions that occur in small diameter resistance arteries. This method can help answer key questions in the cardiovasc
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A cell culture model of resistance arteries is described, allowing for the dissection of signaling pathways in endothelium, smooth muscle, or between endothelium and smooth muscle (the myoendothelial junction). The selective application of agonists or protein isolation, electron microscopy, or immunofluorescence can be utilized using this cell culture model.