The overall goal of this nanotube pulling methodology is to quantitatively study the interactions between specific proteins of interest and highly curved membranes. This method can help answer key question in the biology of cell membranes, such as
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Many proteins in the cell sense and induce membrane curvature. We describe a method to pull membrane nanotubes from lipid vesicles to study the interaction of proteins or any curvature-active molecule with curved membranes in vitro.