September 1st, 2017
•This work presents a step-by-step protocol for the unbiased stereological estimation of dopaminergic neuronal cell numbers in the mouse substantia nigra using standard microscopy equipment (i.e., a light microscope, a motorized object table (x, y, z plane), and public domain software for digital image analysis.
Related Videos
Imaging Odor-Evoked Activities in the Mouse Olfactory Bulb using Optical Reflectance and Autofluorescence Signals
In ovo Electroporation in Chick Midbrain for Studying Gene Function in Dopaminergic Neuron Development
Comprehensive Profiling of Dopamine Regulation in Substantia Nigra and Ventral Tegmental Area
Directed Dopaminergic Neuron Differentiation from Human Pluripotent Stem Cells
The Neuromuscular Junction: Measuring Synapse Size, Fragmentation and Changes in Synaptic Protein Density Using Confocal Fluorescence Microscopy
Optical Control of a Neuronal Protein Using a Genetically Encoded Unnatural Amino Acid in Neurons
Assay to Measure Nucleocytoplasmic Transport in Real Time within Motor Neuron-like NSC-34 Cells
The Optical Fractionator Technique to Estimate Cell Numbers in a Rat Model of Electroconvulsive Therapy
Live Images of GLUT4 Protein Trafficking in Mouse Primary Hypothalamic Neurons Using Deconvolution Microscopy
A Microbiomechanical System for Studying Varicosity Formation and Recovery in Central Neuron Axons
Copyright © 2024 MyJoVE Corporation. 판권 소유