Preparation of Tissue Sections and Methylene Crosslink Hydrolysis
2:40
Tissue Digestion
3:57
Matrix Coating via Sublimation
5:26
Recrystallization and Instrumentation
6:20
Results: Correctly and Incorrectly Prepared Human Brain Tissue
7:07
Conclusion
필기록
The overall goal of this procedure is to reproducibly prepare a tissue sample for peptide mass spectrometry imaging. This method allows novice mass spectrometry users to develop their own imaging mass spectrometry workflows without going through l
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This protocol describes a reproducible and reliable method for the sublimation-based preparation of formalin fixed tissue destined for imaging mass spectrometry.