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Use of Anti-phospho-girdin Antibodies to Visualize Intestinal Tuft Cells in Free-Floating Mouse Jejunum Cryosections

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06:26 min

March 21st, 2018

March 21st, 2018

11,255 Views

0:04

Title

0:40

Dissection and Isolation of Jejunum

2:40

Immunofluorescence of Tuft Cells Using Free-floating Cryosections

4:42

Results: Representative Fluorescence Images of Mouse Intestinal Tuft Cells

5:55

Conclusion

필기록

Kuga et al. discovered that phosphorylation-status specific antibodies against the actin binding protein girdin phosphorylated at tyrosine 1798 (pY1798) can be used to label tuft cells (TCs). This protocol allows robust visualization of TCs using immunofluorescent staining of free-floating jejunum cryosections with pY1798 antibodies.

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