Tissue Sampling for Ribonucleic Acid (RNA) Extraction and Homogenization
3:22
RNA Extraction from Tissue
5:47
Digestion of Remaining DNA
6:51
Results: LPS Injection Induces the Expression of Pro-inflammatory Cytokines in C57Bl/6 Mice
7:43
Conclusion
필기록
The overall goal of this experiment is to present a model that mimics the entrance of microbial-derived products after an intestinal barrier breach. This model can be used to investigate immune responses after invasion of microorganisms. This meth
이 콘텐츠에 액세스하려면 로그인하거나 무료 평가판을 시작하세요
Here a protocol to mimic the entrance of bacterial-derived compounds after intestinal barrier breach is presented. A low sublethal dose of lipopolysaccharide was injected systemically into mice, which were monitored for 24 h post-injection. The expression of pro-inflammatory cytokines was determined at several time points in spleen, liver, and colon.