September 19th, 2018
•Here, we present a method for generating tissue-specific binary transcription systems in Drosophila by replacing the first coding exon of genes with transcription drivers. The CRISPR/Cas9-based method places a transactivator sequence under the endogenous regulation of a replaced gene, and consequently facilitates transctivator expression exclusively in gene-specific spatiotemporal patterns.
Related Videos
Efficient Gene Transfer in Chick Retinas for Primary Cell Culture Studies: An Ex-ovo Electroporation Approach
Genome-wide Snapshot of Chromatin Regulators and States in Xenopus Embryos by ChIP-Seq
Generating CRISPR/Cas9 Mediated Monoallelic Deletions to Study Enhancer Function in Mouse Embryonic Stem Cells
Identification of Critical Conditions for Immunostaining in the Pea Aphid Embryos: Increasing Tissue Permeability and Decreasing Background Staining
Microinjection for Transgenesis and Genome Editing in Threespine Sticklebacks
Dissection and Staining of Drosophila Pupal Ovaries
Application of RNAi and Heat-shock-induced Transcription Factor Expression to Reprogram Germ Cells to Neurons in C. elegans
Thawing, Culturing, and Cryopreserving Drosophila Cell Lines
Hemogenic Reprogramming of Human Fibroblasts by Enforced Expression of Transcription Factors
In Vitro Culture Strategy for Oocytes from Early Antral Follicle in Cattle
Copyright © 2024 MyJoVE Corporation. 판권 소유