July 28th, 2018
•Due to the high lipid content, adipose tissue has been challenging to visualize using traditional histological methods. Adipo-Clear is a tissue clearing technique that allows robust labeling and high-resolution volumetric fluorescent imaging of adipose tissue. Here, we describe the methods for sample preparation, pretreatment, staining, clearing, and mounting for imaging.
Tags
Related Videos
Actin Co-Sedimentation Assay; for the Analysis of Protein Binding to F-Actin
Hi-C: A Method to Study the Three-dimensional Architecture of Genomes.
A System for Culturing Iris Pigment Epithelial Cells to Study Lens Regeneration in Newt
Quantitative Comparison of cis-Regulatory Element (CRE) Activities in Transgenic Drosophila melanogaster
FRET Imaging in Three-dimensional Hydrogels
ACT-PRESTO: Biological Tissue Clearing and Immunolabeling Methods for Volume Imaging
Single-Cell Resolution Three-Dimensional Imaging of Intact Organoids
Exploring Adipose Tissue Structure by Methylsalicylate Clearing and 3D Imaging
Semi-Automated Isolation of the Stromal Vascular Fraction from Murine White Adipose Tissue Using a Tissue Dissociator (Video) | JoVE
Murine Adipose Tissue Excision (Video) | JoVE
Copyright © 2024 MyJoVE Corporation. 판권 소유