Name: portable thermographic screening for detection of acute wallenberg's syndrome
Uploaded: 2019-09-19T12:30:00
Description: Watch this Scientific Journal Video about Portable Thermographic Screening for Detection of Acute Wallenberg's Syndrome at JoVE.com

Not applicable

All methods described here were approved by the Human Research Ethics Committee Institutional Review Board of Kanto Central Hospital.
NOTE: We used a commercially available portable thermal camera and dedicated software (see the Table of Materials), and have constructed our protocols based on the use of these specific instruments.
1. Preparation for Measurements
<ol>
	<li>Charge the thermal camera before use.</li>
	<li>Turn on the...

<ol>
	<li>Kim, J. S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Pure+lateral+medullary+infarction:+Clinical-radiological+correlation+of+130+acute,+consecutive+patients.">Pure lateral medullary infarction: Clinical-radiological correlation of 130 acute, consecutive patients.</a> Brain. 126 (8), 1864-1872 (2003).</li><li>Kameda, W., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Lateral+and+medial+medullary+infarction:+a+comparative+analysis+of+214+patients.">Lateral and medial medullary infarction: a comparative analysis of 214 patients.</a> Stroke. 35 (3), 694-699 (2004).</li><li>Nowak, D. A., Topka, H. R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+clinical+variability+of+Wallenberg's+syndrome:+the+anatomical+correlate+of+ipsilateral+axial+lateropulsion.">The clinical variability of Wallenberg's syndrome: the anatomical correlate of ipsilateral axial lateropulsion.</a> Journal of Neurology. 253 (4), 507-511 (2006).</li><li>Ogawa, K., Suzuki, Y., Oishi, M., Kamei, S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Clinical+study+of+46+patients+with+lateral+medullary+infarction.">Clinical study of 46 patients with lateral medullary infarction.</a> Journal of Stroke and Cerebrovascular Diseases. 24 (5), 1065-1074 (2015).</li><li>Parathan, K. K., Kannan, R., Chitrambalam, P., Aiyappan, S. K., Deepthi, N. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+rare+variant+of+Wallenberg's+syndrome:+Opalski+syndrome.">A rare variant of Wallenberg's syndrome: Opalski syndrome.</a> Journal of Clinical &amp; Diagnostic Research. 8 (7), 8-9 (2014).</li><li>Korpelainen, J. T., Sotaniemi, K. A., Myllyl&#228;, <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Asymmetrical+skin+temperature+in+ischemic+stroke.">Asymmetrical skin temperature in ischemic stroke.</a> Stroke. 26 (9), 1543-1547 (1995).</li><li>Takahashi, M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Utility+of+thermographic+measurements+of+laterality+of+body+surface+temperature+to+prevent+misdiagnosis+of+acute+Wallenberg's+syndrome.">Utility of thermographic measurements of laterality of body surface temperature to prevent misdiagnosis of acute Wallenberg's syndrome.</a> Brain and Behavior. 8, (2018).</li><li>Kim, J. S., Lee, J. H., Lee, M. C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Patterns+of+sensory+dysfunction+in+lateral+medullary+infarction.+Clinical-MRI+correlation.">Patterns of sensory dysfunction in lateral medullary infarction. Clinical-MRI correlation.</a> Neurology. 49 (6), 1557-1563 (1997).</li><li>Glass, T. A., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Outcome+at+30+days+in+the+New+England+Medical+Center+Posterior+Circulation+Registry.">Outcome at 30 days in the New England Medical Center Posterior Circulation Registry.</a> Archives of Neurology. 59 (3), 369-376 (2002).</li><li>Akhtar, N., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Ischaemic+posterior+circulation+stroke+in+State+of+Qatar.">Ischaemic posterior circulation stroke in State of Qatar.</a> European Journal of Neurology. 16 (9), 1004-1009 (2009).</li><li>Fitzek, S., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Time+course+of+lesion+development+in+patients+with+acute+brain+stem+infarction+and+correlation+with+NIHSS+score.">Time course of lesion development in patients with acute brain stem infarction and correlation with NIHSS score.</a> European Journal of Radiology. 39 (3), 180-185 (2001).</li><li>Choi, H. S., Park, S. C., Lee, Y. J., Kang, J. W. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Lateral+medullary+infarction+presenting+with+vertigo+without+other+neurological+signs.">Lateral medullary infarction presenting with vertigo without other neurological signs.</a> Otolaryngology-Head and Neck Surgery. 147 (6), 1162-1163 (2012).</li><li>Oppenheim, C., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=False-negative+diffusion-weighted+MR+findings+in+acute+ischemic+stroke.">False-negative diffusion-weighted MR findings in acute ischemic stroke.</a> American Journal of Neuroradiology. 21 (8), 1434-1440 (2000).</li><li>Tsuyusaki, Y., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=&#34;Invisible&#34;+brain+stem+infarction+at+the+first+day.">&#34;Invisible&#34; brain stem infarction at the first day.</a> Journal of Stroke and Cerebrovascular Diseases. 23 (7), 1903-1907 (2014).</li><li>Tiu, C., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Vertebral+artery+dissection:+a+contemporary+perspective.">Vertebral artery dissection: a contemporary perspective.</a> Maedica. 11 (2), 144-149 (2016).</li><li>Low, P. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=.">.</a> Clinical Autonomic Disorders: Evaluation and Management. , (1997).</li><li>Takahashi, M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Teaching+NeuroImages:+the+half-split+man.">Teaching NeuroImages: the half-split man.</a> Neurology. 87 (11), (2016).</li><li>Vardasco, R., Ring, F., Plassmann, P., Jones, C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Thermal+symmetry+of+the+upper+and+lower+extremities+in+healthy+subjects.">Thermal symmetry of the upper and lower extremities in healthy subjects.</a> Thermology International. 22 (2), 53-60 (2012).</li><li>Nakazato, Y., Shimazu, K., Tamura, N., Hamaguchi, K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+study+of+skin+surface+temperature+in+patients+with+unilateral+cerebral+infarction--with+special+reference+to+central+autonomic+regulation+of+skin+vasomotor+response.">A study of skin surface temperature in patients with unilateral cerebral infarction--with special reference to central autonomic regulation of skin vasomotor response.</a> Clinical Neurology. 35 (7), 758-763 (1995).</li><li>Motobe, K., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Cut-off+value+of+the+ankle-brachial+pressure+index+at+which+the+accuracy+of+brachial-ankle+pulse+wave+velocity+measurement+is+diminished.">Cut-off value of the ankle-brachial pressure index at which the accuracy of brachial-ankle pulse wave velocity measurement is diminished.</a> Circulation Journal. 69 (1), 55-60 (2005).</li><li>International Academy of Clinical Thermography. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=.">.</a> Thermography Guidelines: Standards and protocols in clinical thermographic imaging. , (2002).</li></ol>

Critical steps of this protocol are the establishment the initial suspicion of WS and the decision to acquire thermographic images of the patient. Acquiring thermographic images and assessing the laterality of BST is a simple approach, even for non-neurologists who may examine patients upon initial presentation to the emergency department or a general clinic. If a patient exhibits laterality of BST, especially at multiple locations on the same side, the physician should consider the possibility of WS. Because most WS pat...

Wallenberg&#39;s syndrome (WS) is a type of brainstem infarction. Acute WS patients are sometimes initially misdiagnosed with non-stroke diseases because of the symptomatic and magnetic resonance imaging (MRI) characteristics of WS. To accurately diagnose acute WS, careful neurological examination is necessary, which may be difficult for non-neurologists to whom affected patients initially present. Here, we present a simple, rapid, noninvasive, and cost-effective method for the detection of acute WS using portable thermography.
WS is caused by the infarction of a wedge of the dorsal lateral medulla oblongata, due to occlusion of the vertebral artery or posterior inferior cerebellar artery1,2. WS may be misdiagnosed as a non-stroke disease because of a combination of unique symptomatic and MRI characteristics that contrast with those typically observed in cerebral infarction. Hemiparesis and tactile sensory disturbance, which tend to be observed in patients with other types of cerebral infarction, are rare in WS patients; however, they exhibit various combinations of clinical symptoms, including hoarseness and dysphagia, dissociated sensory disturbance, vertigo, gaze-induced nystagmus, ataxia, and Horner&#39;s syndrome1,2,3,4,5,6,7,. Another unique characteristic of WS patients is the limited severity of symptoms, which is similar to that in other types of brainstem infarctions7,8,9,10,11. Some patients with brainstem infarctions have arrived at the outpatient clinic on foot and reported only minor complaints7. In some patients with WS, vertigo is the only presenting symptom, and it can therefore be difficult to differentiate between WS and auditory vertigo12. Furthermore, WS can affect young patients, due to its potential etiology of artery dissection2. MRI analysis of brainstem infarction, including WS, is unique in that the high-intensity diffusion-weighted imaging signal may be delayed in some patients7,13,14.
The above characteristics are thought to cause misdiagnosis of WS. Dysphagia may cause aspiration pneumonia or asphyxia, and artery dissection may cause subarachnoid hemorrhage15; therefore, overlooking WS may result in the development of life-threatening conditions for the patient. Although careful neurological examination is necessary to prevent the misdiagnosis of WS, it is likely that a patient will first present to a non-neurologist. Therefore, a rapid and simple method for screening of acute WS may be clinically useful.
Previously, we reported that 89% of acute WS patients exhibit laterality of BST, which is presumed to result from disturbance of the central autonomic nervous tract due to infarction at the lateral medulla7. Because this autonomic nervous tract descends from the lateral brainstem (including the ventro-lateral medulla) and contains the connective pathway of sweating and skin blood flow16, disturbance of sweating and vasoconstriction lead to increased BST on the ipsilateral side of WS. In the prior report, we also showed that the laterality of BST can be easily detected within 2 min using thermographic measurement in most patients with WS7,17. Here, we report a method for the detection of laterality of BST using thermography, which may be useful in preventing misdiagnosis of acute WS.

<table border="1" fo:keep-together.within-page="1" fo:keep-with-next.within-page="always">
	<tbody>
		<tr>
			<td>FLIR E5</td>
			<td>FLIR Systems</td>
			<td>P/N: 63905-0501</td>
			<td></td>
		</tr>
		<tr>
			<td>FLIR Tools</td>
			<td>FLIR Systems</td>
			<td></td>
			<td>RRID:SCR_016330</td>
		</tr>
	</tbody>
</table>

portable thermographic screening for detection of acute wallenberg's syndrome

Wallenberg&#39;s syndrome (WS) is a type of brainstem infarction. WS patients often show Horner&#39;s syndrome, dissociated sensory disturbance, truncal ataxia, and hoarseness. However, they rarely show tactile sensory disturbance and paralysis of the extremities. Additionally, acute brainstem infarction is often not apparent in magnetic resonance images. These symptomatic and imaging characteristics sometimes lead to misdiagnosis of WS as a non-stroke disease, including auditory vertigo. Although careful neurological examination is necessary to prevent misdiagnosis of WS, this type of examination may be difficult for non-neurologists to whom affected patients initially present. Lateral differences in body surface temperature (BST) constitute a recognized and widespread symptom of WS. We previously reported that most acute WS patients exhibit lateral differences in BST at multiple locations and that these lateral differences in BST could easily be detected by thermographic measurement. Here, we present the method for use of portable thermography to detect acute WS, using a simple, rapid, noninvasive, and cost-effective approach. To assess lateral differences in BST among patients with suspected WS, BST was measured as soon as possible in the examination room or in the patient&#39;s bedroom. Measurements were performed bilaterally at four locations where images could easily be acquired (face, palm of the hand, abdomen, and dorsum of the foot) using a portable thermal camera. When lateral differences in BST are observed macroscopically, especially in multiple locations on the same side, a diagnosis of WS should be suspected. Macroscopic assessment of BST laterality can be made within 2 min of the acquisition of thermographic images. This method may be useful in preventing misdiagnosis of acute WS as a non-stroke disease, especially when such patients initially present to non-neurologists.

Acquisition of thermographic images and macroscopic assessment to determine whether BST exhibits laterality may be performed within 2 min in most patients. Most acute WS patients exhibit laterality of BST at multiple locations. Some patients exhibit laterality of BST throughout the body (Figure 2A), whereas some exhibit laterality only in a few locations (Figure 2B). The warmer side, as determine...

Watch this Scientific Journal Video about Portable Thermographic Screening for Detection of Acute Wallenberg's Syndrome at JoVE.com

Portable Thermographic Screening for Detection of Acute Wallenberg's Syndrome

Acute Wallenberg&#39;s syndrome can be misdiagnosed as a non-stroke disease, such as auditory vertigo. Thus, careful neurological examination, which is sometimes difficult for non-neurologists, is necessary for precise diagnosis. Here, we present a simple, rapid, noninvasive, and cost-effective method for detection of acute Wallenberg&#39;s syndrome using portable thermography.

Wallenberg&#39;s syndrome (WS) is a type of brainstem infarction. WS patients often show Horner&#39;s syndrome, dissociated sensory disturbance, truncal ...

Wallenberg's syndrome is caused by the infarction of the lateral medulla and is sometimes misdiagnosed as non-stroke disease. Using portable thermography may help prevent the misdiagnosis of this neurological condition. This method is a very simple, rapid, non-invasive and cost-effective technique that allows the possibility of Wallenberg's syndrome to be easily determined.Before beginning the measurements, charge and turn on the thermal camera. Press the center button on the camera to display the menu and select the color in iron or rainbow. Then press the center button on the camera to display the menu again and select measurement and center spot measurement to allow measurement of the body surface temperature in real time.For Wallenberg's syndrome screening select patients with dizziness, vertigo, ptosis, hoarseness, dysphagia, and isochoria or dissociated sensory disturbance as all of the symptoms have been associated with Wallenberg's syndrome. It is important to measure the body surface temperature by thermography in any patient in which Wallenberg's syndrome is suspected. To subjectively determine the laterality of the body surface temperature first palpate patients with suspected Wallenberg's syndrome.To acquire thermographic images instruct the patient to remove their socks and shoes and have the patient remove enough clothing to enable examination of the abdominal region as per their consent. As soon as Wallenberg's syndrome is suspected have the patient assume a supine posture, or sitting position, and have a photographer hand 50 to 100 centimeters from the patient. Then have the photographer acquire one image each at the frontal face, bilateral palm, abdomen, and bilateral dorsum of the foot.Consider body surface temperature to exhibit laterality when the body surface temperature between the right and left sides is markedly different macroscopically and the degree of laterality is greater than a half of a degree Celsius. If laterality of the body surface temperature is not obvious or appears to be less than half of a degree Celsius initialize the thermal imaging and analysis software and open the acquired images to be analyzed. Use the ellipse measurement button to indicate ellipses with diameters of greater than five millimeters at that nasolabial fold on the face, the palm of the hand, the thoracic spine levels eight to 10 approximately five centimeters from the umbilicus of the torso, and at the center of the dorsum of the foot.Then measure the average body surface temperature from each location and compare it to the body surface temperature on the contralateral side of the body. Some patients exhibit laterality of the body surface temperature throughout the body, whereas some exhibit laterality in only a few locations. The warmer side, as determined by the body surface temperature, is ipsolateral to the location of Wallenberg's syndromes.Importantly however, a Wallenberg's syndrome patient with a very small infarction may not exhibit body surface temperature laterality. When a patient does not exhibit a central nervous disorder laterality of the body surface temperature is typically not observed. However, when a patient exhibits vascular stenosis the body surface temperature maybe be lower in the extremities with vascular stenosis than in extremities on the contralateral side, and these patients may exhibit vascular stenosis in both the upper and lower limbs.The precise degree of the body surface temperature can be analyzed with dedicated software and may be required when the laterality of the body surface temperature is not apparent macroscopically. It is important to remember that this method is used for the screening of Wallenberg's syndrome, not for confirming the diagnosis of Wallenberg's syndrome. To confirm a Wallenberg's syndrome diagnosis a careful neurological examination and repeated brain stem MRIs are needed.Consider hospitalization when the possibility of Wallenberg's syndrome cannot be excluded.

Research

JoVE Journal

Neuroscience

Preparation of Acute Subventricular Zone Slices for Calcium Imaging

The subventricular zone (SVZ) is one of the two neurogenic zones in the postnatal brain. The SVZ contains densely packed cells, including neural ...

Dual Electrophysiological Recordings of Synaptically-evoked Astroglial and Neuronal Responses in Acute Hippocampal Slices

Astrocytes form together with neurons tripartite synapses, where they integrate and modulate neuronal activity. Indeed, astrocytes sense neuronal inputs ...

In vivo Postnatal Electroporation and Time-lapse Imaging of Neuroblast Migration in Mouse Acute Brain Slices

In vivo Postnatal Electroporation and Time-lapse Imaging of Neuroblast Migration in Mouse Acute Brain Slices

The subventricular zone (SVZ) is one of the main neurogenic niches in the postnatal brain. Here, neural progenitors proliferate and give rise to ...

Prolonged Incubation of Acute Neuronal Tissue for Electrophysiology and Calcium-imaging

Acute neuronal tissue preparations, brain slices and retinal wholemount, can usually only be maintained for 6 - 8 h following dissection. This limits the ...

Optimized Management of Endovascular Treatment for Acute Ischemic Stroke

This manuscript describes a streamlined protocol for the management of patients with acute ischemic stroke, which aims at the minimization of time from ...

Assay Development for High Content Quantification of Sod1 Mutant Protein Aggregate Formation in Living Cells

Amyotrophic lateral sclerosis (ALS) is a fatal neurodegenerative disease that can be caused by inherited mutations in the gene encoding copper-zinc ...

Screening of Axonal Degeneration in Carpal Tunnel Syndrome Using Ultrasonography and Nerve Conduction Studies

Axonal degeneration, indicative of surgical decompression, may coexist in carpal tunnel syndrome (CTS) as the disease progresses. However, the current ...

Preparation of Acute Human Hippocampal Slices for Electrophysiological Recordings

Epilepsy affects about 1% of the world population and leads to a severe decrease in quality of life due to ongoing seizures as well as high risk for ...

Detection of Abnormal Prion Protein by Immunohistochemistry

Abnormal prion proteins (PrPSc) are the disease-associated isoform of cellular prion protein and diagnostic markers of transmissible spongiform ...

Ultrasound-Guided Needle Release Combined with Corticosteroid Injection

Ultrasound-Guided Needle Release Combined with Corticosteroid Injection for the Treatment of Supinator Syndrome

Author Spotlight: Ultrasound-Guided Needle Release Combined with Corticosteroid Injection for the Treatment of Supinator Syndrome  

The two main branches of the radial nerve (RN) are the deep branch (DBRN) and the superficial branch (SBRN). The RN splits into two main branches at the ...