Labeling of S phase by IdU Incorporation and Isolation of Cells
5:19
Cisplatin Labeling and Cell Fixation
6:56
Barcoding of Samples
8:02
Labeling of Cells for Mass Cytometry
9:48
Results: Cell Cycle Specific Analysis of Cellular Protein Expression by Mass Cytometry
11:51
Conclusion
필기록
This protocol is significant for two main reasons. Number one, it allows a user to be able to determine the cell cycle states of skin cells that have been isolated from an animal model. It also allows us to be able to analyze protein expression in
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This protocol describes how to isolate skin keratinocytes from mouse models, to stain with metal-tagged antibodies, and to analyze stained cells by mass cytometry in order to profile the expression pattern of proteins of interest in the different cell cycle phases.