August 4th, 2016
•This report describes a CUBIC protocol to clarify full thickness mouse skin biopsies, and visualize protein expression patterns, proliferating cells, and sebocytes at the single cell resolution in 3D. This method enables accurate assessment of skin anatomy and pathology, and of abnormal epidermal phenotypes in genetically modified mouse lines.
Tags
Related Videos
Method for Whole Mount Antibody Staining in Chick
Zebrafish Whole Mount High-Resolution Double Fluorescent In Situ Hybridization
Staining of Proteins in Gels with Coomassie G-250 without Organic Solvent and Acetic Acid
Whole-mount Immunohistochemical Analysis for Embryonic Limb Skin Vasculature: a Model System to Study Vascular Branching Morphogenesis in Embryo
Studying Proteolysis of Cyclin B at the Single Cell Level in Whole Cell Populations
RNA In situ Hybridization in Whole Mount Embryos and Cell Histology Adapted for Marine Elasmobranchs
High Resolution Whole Mount In Situ Hybridization within Zebrafish Embryos to Study Gene Expression and Function
Analysis of Global RNA Synthesis at the Single Cell Level following Hypoxia
An Efficient Method for Quantitative, Single-cell Analysis of Chromatin Modification and Nuclear Architecture in Whole-mount Ovules in Arabidopsis
Visualizing Clathrin-mediated Endocytosis of G Protein-coupled Receptors at Single-event Resolution via TIRF Microscopy
Copyright © 2024 MyJoVE Corporation. 판권 소유