Ex Vivo Culture of FACS Sorted Hematopoietic Stem Cells
4:00
Measurement of Mitochondrial Mass and Membrane Potential
6:21
Results: Mitochondrial Profiles
7:18
Conclusion
필기록
This protocol is a simple flow cytometry-based assay allowing measurement of mitochondrial membrane potential and mitochondrial mass in living cells. Standard metabolic assays fail when working with rare populations such as the hematopoietic stem
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Here we describe a reliable method to measure mitochondrial mass and membrane potential in ex vivo cultured hematopoietic stem cells and T cells.