Determining Virus Titer and Transduction Efficacy with Flow Cytometry
4:27
Lentiviral Transduction of Target Cells and Conditional Induction of Cas9 Mediated Gene Editing
6:06
Results: Analysis of Reversibility and Rapidity of Destabilized DD-Cas9 Protein
7:24
Conclusion
필기록
This method provides fast, temperature controlled CRISPR-Cas9 gene editing under a small molecule, Shield-1. It also offers less off-target effects, lower cell toxicity, and also higher internally controlled gene editing when comparing it to const
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Here, we describe a genome-editing tool based on the temporal and conditional stabilization of clustered regularly interspaced short palindromic repeat- (CRISPR-) associated protein 9 (Cas9) under the small molecule, Shield-1. The method can be used for cultured cells and animal models.