Cardiovascular disease remains the number one cause of death worldwide. Traditional in vitro models rely on monolayer culture. However, the heart, specifically the heart muscle or the myocardium, is complex in its 3D anisotropy and cellular compos
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The goal of this protocol is to explain and demonstrate the development of a three-dimensional (3D) microfluidic model of highly aligned human cardiac tissue, composed of stem cell-derived cardiomyocytes co-cultured with cardiac fibroblasts (CFs) within a biomimetic, collagen-based hydrogel, for applications in cardiac tissue engineering, drug screening, and disease modeling.