Results: Isolation of Subcellular Compartments of Murine Rod Photoreceptors for Protein Analysis
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Conclusion
필기록
The two peeling techniques demonstrated here isolate individual rod subcellular compartments, and can help reveal the important physiologic processes occurring in each specialized compartment in healthy and diseased rods. Isolating different rod c
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This protocol presents two techniques to isolate the subcellular compartments of murine rod photoreceptors for protein analysis. The first method utilizes live retinae and cellulose filter paper to separate rod outer segments, while the second employs lyophilized retinae and adhesive tape to peel away rod inner and outer segment layers.