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Single-cell Profiling of Developing and Mature Retinal Neurons

DOI :

10.3791/3824-v

April 19th, 2012

April 19th, 2012

13,980 Views

1Department of Genetics, Development and Cell Biology, Neuroscience Program, Iowa State University

A method for the isolation of single retinal cells and subsequent amplification of their cDNAs is described. Single-cell transcriptomics reveals the degree of cellular heterogeneity present in a tissue and uncovers new marker genes for rare cell populations. The accompanying protocol can be adjusted to suit many different cell types.

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Single cell Profiling

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