JoVE Table of Contents: Issue 181, March 2022

A Procedure to Study Stress-Induced Relapse of Heroin Seeking after Punishment-Imposed Abstinence

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Dziennik (Behavior)

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Methods Collections

Assessment of Mouse Judgment Bias through an Olfactory Digging Task

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Dziennik (Behavior)

Exploring Biomolecular Interaction Between the Molecular Chaperone Hsp90 and Its Client Protein Kinase Cdc37 using Field-Effect Biosensing Technology

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Dziennik (Biochemistry)

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Methods Collections

Determination of Glucan Chain Length Distribution of Glycogen Using the Fluorophore-Assisted Carbohydrate Electrophoresis (FACE) Method

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Dziennik (Biochemistry)

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Methods Collections

Bacterial Expression and Purification of Human Matrix Metalloproteinase-3 using Affinity Chromatography

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Dziennik (Biochemistry)

Identification of Hemolytic and Phospholipase Activity in Crude Extracts from Sea Anemones by Straightforward Bioassays

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Dziennik (Biochemistry)

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Methods Collections

Fingerprinting Cardiolipin in Leukocytes by Mass Spectrometry for a Rapid Diagnosis of Barth Syndrome

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Dziennik (Biochemistry)

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Methods Collections

Production of siRNA-Loaded Lipid Nanoparticles using a Microfluidic Device

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Dziennik (Biochemistry)

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Methods Collections

Purification of Ubiquitinated p53 Proteins from Mammalian Cells

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Dziennik (Biochemistry)

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Methods Collections

Quantification of Humic and Fulvic Acids in Humate Ores, DOC, Humified Materials and Humic Substance-Containing Commercial Products

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Dziennik (Biochemistry)

Routine Collection of High-Resolution cryo-EM Datasets Using 200 KV Transmission Electron Microscope

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Dziennik (Biochemistry)

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Methods Collections

Förster Resonance Energy Transfer Mapping: A New Methodology to Elucidate Global Structural Features

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Dziennik (Biochemistry)

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Methods Collections

Alleviation of Diabetic Tendon Injury via Activation of Tendon Fibroblasts Autophagy under Berberine Treatment

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Dziennik (Biochemistry)

$On-Chip Crystallization and Large-Scale Serial Diffraction at Room Temperature$

On-Chip Crystallization and Large-Scale Serial Diffraction at Room Temperature

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Dziennik (Biochemistry)

Deciphering Molecular Mechanism of Histone Assembly by DNA Curtain Technique

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Dziennik (Biochemistry)

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Methods Collections

<em>Ex Vivo</em> Hepatic Perfusion Through the Portal Vein in Mouse

Ex Vivo Hepatic Perfusion Through the Portal Vein in Mouse

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Dziennik (Biochemistry)

Analysis of Lipid Signaling in <em>Drosophila</em> Photoreceptors using Mass Spectrometry

Analysis of Lipid Signaling in Drosophila Photoreceptors using Mass Spectrometry

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Dziennik (Biochemistry)

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Methods Collections

A Reliable Porcine Fascio-Cutaneous Flap Model for Vascularized Composite Allografts Bioengineering Studies

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Dziennik (Bioengineering)

Large-Scale, Automated Production of Adipose-Derived Stem Cell Spheroids for 3D Bioprinting

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Dziennik (Bioengineering)

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Methods Collections

An Open Source Technology Platform to Manufacture Hydrogel-Based 3D Culture Models in an Automated and Standardized Fashion

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Dziennik (Bioengineering)

Light-Controlled Fermentations for Microbial Chemical and Protein Production

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Dziennik (Bioengineering)

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Methods Collections

A Micropatterning Assay for Measuring Cell Chirality

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Dziennik (Bioengineering)

Computational Reconstruction of Pancreatic Islets as a Tool for Structural and Functional Analysis

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Dziennik (Bioengineering)

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Methods Collections

Laser Microdissection for Species-Agnostic Single-Tissue Applications

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Dziennik (Biology)

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Methods Collections

Automated Charting of the Visual Space of Housefly Compound Eyes

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Dziennik (Biology)

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Methods Collections

Mass Production of Entomopathogenic Fungi, <em>Metarhizium robertsii</em> and <em>Metarhizium pinghaense</em>, for Commercial Application Against Insect Pests

Mass Production of Entomopathogenic Fungi, Metarhizium robertsii and Metarhizium pinghaense, for Commercial Application Against Insect Pests

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Dziennik (Biology)

Quantitative Analysis of Viscoelastic Properties of Red Blood Cells Using Optical Tweezers and Defocusing Microscopy

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Dziennik (Biology)

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Methods Collections

An Improved Chemotaxis Assay for the Rapid Identification of Rhizobacterial Chemoattractants in Root Exudates

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Dziennik (Biology)

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Methods Collections

Establishing Human Lung Organoids and Proximal Differentiation to Generate Mature Airway Organoids

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Dziennik (Biology)

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Methods Collections

Assessing Energy Substrate Oxidation <em>In Vitro</em> with <sup>14</sup>CO<sub>2</sub> Trapping

Assessing Energy Substrate Oxidation In Vitro with ¹⁴CO₂ Trapping

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Dziennik (Biology)

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Methods Collections

Construction of Homozygous Mutants of Migratory Locust Using CRISPR/Cas9 Technology

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Dziennik (Biology)

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Methods Collections

Removal of an Internal Translational Start Site from mRNA While Retaining Expression of the Full-Length Protein

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Dziennik (Biology)

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Methods Collections

Isolation and Functional Analysis of Arteriolar Endothelium of Mouse Brain Parenchyma

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Dziennik (Biology)

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Methods Collections

Application of RNA Interference in the Pinewood Nematode, <em>Bursaphelenchus xylophilus</em>

Application of RNA Interference in the Pinewood Nematode, Bursaphelenchus xylophilus

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Dziennik (Biology)

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Methods Collections

Integrative Toolkit to Analyze Cellular Signals: Forces, Motion, Morphology, and Fluorescence

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Dziennik (Biology)

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Methods Collections

Exploring m<sup>6</sup>A and m<sup>5</sup>C Epitranscriptomes upon Viral Infection: an Example with HIV

Exploring m⁶A and m⁵C Epitranscriptomes upon Viral Infection: an Example with HIV

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Dziennik (Biology)

A Flame-Free Method for Sterilizing <em>C. elegans</em> Picks, Spatulas, and Scalpels

A Flame-Free Method for Sterilizing C. elegans Picks, Spatulas, and Scalpels

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Dziennik (Biology)

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Methods Collections

Nitroreductase/Metronidazole-Mediated Ablation and a MATLAB Platform (RpEGEN) for Studying Regeneration of the Zebrafish Retinal Pigment Epithelium

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Dziennik (Biology)

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Methods Collections

Zebrafish Corneal Wound Healing: From Abrasion to Wound Closure Imaging Analysis

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Dziennik (Biology)

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Methods Collections

A Highly Scalable Approach to Perform Ecological Surveys of Selfing <em>Caenorhabditis</em> Nematodes

A Highly Scalable Approach to Perform Ecological Surveys of Selfing Caenorhabditis Nematodes

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Dziennik (Biology)

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Methods Collections

Structure-Based Simulation and Sampling of Transcription Factor Protein Movements along DNA from Atomic-Scale Stepping to Coarse-Grained Diffusion

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Dziennik (Biology)

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Methods Collections

Effective Oral RNA Interference (RNAi) Administration to Adult <em>Anopheles gambiae</em> Mosquitoes

Effective Oral RNA Interference (RNAi) Administration to Adult Anopheles gambiae Mosquitoes

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Dziennik (Biology)

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Methods Collections

Using Real-Time Cell Metabolic Flux Analyzer to Monitor Osteoblast Bioenergetics

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Dziennik (Biology)

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Methods Collections

Reverse Genetic Approach to Identify Regulators of Pigmentation using Zebrafish

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Dziennik (Biology)

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Methods Collections

Real-Time Detection and Capture of Invasive Cell Subpopulations from Co-Cultures

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Dziennik (Cancer Research)

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Methods Collections

Positron Emission Tomography-based Dose Painting Radiation Therapy in a Glioblastoma Rat Model using the Small Animal Radiation Research Platform

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Dziennik (Cancer Research)

Gelatin Zymography to Detect Gelatinase Activity in Melanoma Cells

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Dziennik (Cancer Research)

A Robust Discovery Platform for the Identification of Novel Mediators of Melanoma Metastasis

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Dziennik (Cancer Research)

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Methods Collections

Uncovering Hidden Dynamics of Natural Photonic Structures Using Holographic Imaging

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Dziennik (Chemistry)

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Methods Collections

Identification and Quantification of Decomposition Mechanisms in Lithium-Ion Batteries; Input to Heat Flow Simulation for Modeling Thermal Runaway

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Dziennik (Chemistry)

Atomic Force Microscopy to Study the Physical Properties of Epidermal Cells of Live <em>Arabidopsis</em> Roots

Atomic Force Microscopy to Study the Physical Properties of Epidermal Cells of Live Arabidopsis Roots

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Dziennik (Developmental Biology)

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Methods Collections

Primary Cell Cultures to Study the Regeneration Potential of Murine Müller Glia after MicroRNA Treatment

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Dziennik (Developmental Biology)

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Methods Collections

2D and 3D Human Induced Pluripotent Stem Cell-Based Models to Dissect Primary Cilium Involvement during Neocortical Development

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Dziennik (Developmental Biology)

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Methods Collections

Targeted Laser Ablation in the Embryo of <em>Saccharina latissima</em>

Targeted Laser Ablation in the Embryo of Saccharina latissima

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Dziennik (Developmental Biology)

The Traceable Calibration of Thrust Stand by Electrostatic Force

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Dziennik (Engineering)

Mass-Rearing and Molecular Studies in Tortricidae Pest Insects

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Dziennik (Environment)

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Methods Collections

Methodology to Test Control Agents and Insecticides Against the Coffee Berry Borer <em>Hypothenemus hampei</em>

Methodology to Test Control Agents and Insecticides Against the Coffee Berry Borer Hypothenemus hampei

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Dziennik (Environment)

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Methods Collections

Mistletoe Eradicator - A Novel Tool for Simultaneous Mechanical and Chemical Control of Mistletoe

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Dziennik (Environment)

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Methods Collections

Inoculation Strategies to Infect Plant Roots with Soil-Borne Microorganisms

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Dziennik (Environment)

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Methods Collections

Using Next Generation Sequencing to Identify Mutations Associated with Repair of a CAS9-induced Double Strand Break Near the CD4 Promoter

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Dziennik (Genetics)

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Methods Collections

An<em> In Vitro</em> Approach to Study Mitochondrial Dysfunction: A Cybrid Model

An In Vitro Approach to Study Mitochondrial Dysfunction: A Cybrid Model

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Dziennik (Genetics)

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Methods Collections