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A Highly Scalable Approach to Perform Ecological Surveys of Selfing Caenorhabditis Nematodes

2.4K Views

09:10 min

March 1st, 2022

DOI :

10.3791/63486-v

March 1st, 2022


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Caenorhabditis Nematodes

Rozdziały w tym wideo

0:04

Introduction

0:34

Collection Preparation

1:21

Field Collection

2:21

Plating Out Field Collections and Isolating Nematodes from Collections

4:06

Exporting S-Plates from Fulcrum

4:41

Check for Proliferation on S-Plates

5:26

Lysis of Isofemale Lines

6:22

PCR of SSU and ITS2 Sequences

7:00

Identifying Nematodes and Processing the Collection Data

8:04

Results: Identifying Nematodes with Sanger Sequencing and Sequence BLAST

8:40

Conclusion

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