Study of Dendritic Cell Development by Short Hairpin RNA-Mediated Gene Knockdown in a Hematopoietic Stem and Progenitor Cell Line In vitro
3.3K Views
•
06:12 min
•
March 7th, 2022
DOI :
March 7th, 2022
•Rozdziały w tym wideo
0:04
Introduction
0:43
Preparation of Immortalized HematopoieticStem and Progenitor Cell (iHSPC) Lines and Lentiviral Transduction
2:08
In Vitro Differentiation of the Stable Knockdown iHSPC Cell Lines
3:05
Flow Cytometric Analysis of the Differentiated Dendritic Cells
4:01
Results: Dendritic Cell Differentiation Following Short Hairpin RNA-Mediated Tcf4 and Id2 Knockdown
5:33
Conclusion
Powiązane Filmy
Generation of Multivirus-specific T Cells to Prevent/treat Viral Infections after Allogeneic Hematopoietic Stem Cell Transplant
17.1K Views
Analysis of Pulmonary Dendritic Cell Maturation and Migration during Allergic Airway Inflammation
14.9K Views
PRP as a New Approach to Prevent Infection: Preparation and In vitro Antimicrobial Properties of PRP
21.2K Views
A Tetracycline-regulated Cell Line Produces High-titer Lentiviral Vectors that Specifically Target Dendritic Cells
14.4K Views
Identifying DNA Mutations in Purified Hematopoietic Stem/Progenitor Cells
12.9K Views
Assessing the Development of Murine Plasmacytoid Dendritic Cells in Peyer's Patches Using Adoptive Transfer of Hematopoietic Progenitors
11.1K Views
Development and Identification of a Novel Subpopulation of Human Neutrophil-derived Giant Phagocytes In Vitro
15.1K Views
A Suction Blister Protocol to Study Human T-cell Recall Responses In Vivo
11.9K Views
Simultaneous Study of the Recruitment of Monocyte Subpopulations Under Flow In Vitro
6.8K Views
Induced Differentiation of M Cell-like Cells in Human Stem Cell-derived Ileal Enteroid Monolayers
8.5K Views
Copyright © 2025 MyJoVE Corporation. Wszelkie prawa zastrzeżone