Preparation of Enantiopure Non-Activated Aziridines and Synthesis of Biemamide B, D, and epiallo-Isomuscarine

Podsumowanie

In this study, we prepare both enantiomers of aziridine-2-carboxylate, which are used in the asymmetric synthesis of alkaloids, including biemamide B and D, and (-)-epiallo-isomuscarine.

Streszczenie

Nitrogen-containing heterocycle aziridines are synthetically very valuable for the preparation of azacyclic and acyclic molecules. However, it is very difficult and laborious to make aziridines in optically pure forms on a large scale to apply asymmetric synthesis of aza compounds. Fortunately, we successfully achieved both enantiomers (2R)- and (2S)-aziridine-2-carboxylates with the electron-donating α-methylbenzyl group at the ring nitrogen as non-activated aziridines. These starting aziridines have two distinct functional groups-highly reactive three-membered ring and versatile carboxylate. They are applicable in ring-opening or ring-transformation with aziridine and in functional group transformation to others from carboxylate. Both of these enantiomers were utilized in the preparation of biologically important amino acyclic and/or aza-heterocyclic compounds in an asymmetric manner. Specifically, this report describes the first expedient asymmetric synthesis of both enantiomers of 5, 6-dihydrouracil-type marine natural products biemamide B and D as potential TGF-β inhibitors. This synthesis consisted of regio- and the stereoselective ring-opening reaction of aziridine-2-carboxylate and subsequent formation of 4-aminoteterahydropyrimidine-2,4-dione. One more example in this protocol dealt a highly stereoselective Mukaiyama reaction of aziridine-2-carboxylate and silyl enol ether, following intramolecular aziridine ring-opening to provide easy and facile access to (-)-epiallo-isomuscarine.

Wprowadzenie

Small rings consisting of cyclopropanes, oxiranes, and aziridines are found in various compounds such as natural products and drugs^1,2. They are primarily used as starting materials exploiting their ring strain. Among the three-ring compounds, aziridine has been studied less extensively due to its instability and uncontrollable reactivity³. As shown in the electrostatic potential maps (Figure 1), a group attached to the aziridine ring-nitrogen, whether electron-donating or electron-attracting, makes the basicity of nitrogen different. This difference provides a striking contrast to the reactivity and selectivity of the corresponding aziridines.

Figure 1: Chemical structures of "activated" and "non-activated" aziridines and electrostatic potential maps of their representative examples N-methylaziridine, and N-acetylaziridine⁴. This figure has been modified with permission from Ranjith et al.⁴. Please click here to view a larger version of this figure.

When the ring nitrogen has an electron-withdrawing group, such as sulfonate, phosphonate, and carbamate, we call it "activated" aziridine. This is readily reactive with nucleophiles to compensate for its instability with a limited scope of regiochemistry. These activated aziridines are prepared through various catalytic methods and used as a starting material. Much of recent aziridine chemistry has dealt with these activated aziridines. However, activated aziridines suffer certain restrictions resulting from their instability and limited reaction scope of the ring opening. On the other hand, aziridines bearing electron-donating substituents, like alkyl or substituted alkyl groups, at the ring nitrogen called "non-activated"⁴, are relatively stable under most circumstances and can be left on the bench for a long time without significant decomposition. The nucleophilic ring-opening reactions of non-activated aziridine occur via the formation of aziridinium ions. Most reactions of aziridine ring-opening and ring transformations proceed in a highly regiochemical manner. However, very few literature reports discuss the preparation of optically pure non-activated aziridines with substituents at the C2 or C3 positions^5,6.

This paper shows the successful preparation of α-methylbenzyl group-containing chiral aziridine-2-carboxylate derivatives, specifically (-)-mentholyl (1R)-phenylethylaziridine-2-carboxylates as its diastereomeric mixture, from the reaction of 2,3-dibromopropionate and (1R)-phenylethylamine. From this diastereomeric mixture, enantiopure (1R)-phenylethyl-(2R)- and (2S)-aziridine-2-carboxylates as their (-)-mentholyl esters were obtained in optically pure forms by selective recrystallization from MeOH and n-pentane on multi-hundred-kilo scales (Figure 1)⁷. These (-)-mentholyl esters can be easily converted into their ethyl or methyl esters by transesterification in the presence of magnesium or potassium carbonate⁷. These compounds can also be prepared easily on a laboratory scale from the reactions of alkyl 2,3-dibromopropionates or the vinyl triflate of α-ketoester with chiral 2-phenylethylamine followed by separation of the diastereomeric mixture using simple flash column chromatography⁸.

Once we have enantiopure chiral aziridine-2-carboxylate, we can synthesize various cyclic and acyclic nitrogen-containing biologically important target molecules based on functional group transformations of carboxylate and highly regio- and stereoselective aziridine-ring opening reactions^6,9,10. The first expedient asymmetric synthesis was applied for both enantiomers of 5, 6-dihydrouracil-type marine natural products biemamide B and D as potential TGF-β inhibitors^11,12. Secondly, the diastereoselective synthesis of β-(aziridin-2-yl)-β-hydroxy ketones was achieved by Mukaiyama aldol reaction of optically pure 1-(1-phenylethyl)-aziridine-2-carboxaldehyde and various enol silanes in the presence of ZnCl₂, in high yield (>82%) with almost perfect stereoselectivity (98:2 dr) via a chelation-controlled transition state. These were used for the asymmetric synthesis of epiallo-isomuscarine alkaloids^13,14,15.

Protokół

1. Synthesis of the diastereomeric mixture of chiral aziridine (-)-mentholyl ester derivative (1)

1. Add 2,3-dibromopropane (-)-menthol ester 1a (5.0 g, 13.58 mM, 1.0 equiv) and a magnetic stirring bar into an oven-dried 250 mL two-neck round-bottom flask under nitrogen (N₂) atmosphere.
2. Add anhydrous acetonitrile (60 mL) to the reaction flask using an airtight syringe.
3. Then cool the reaction mixture at 0 °C using an ice bath and stir the reaction mixture for 5 min.
4. Add potassium carbonate (5.6 g, 40.74 mM, 3.0 equiv) into the reaction mixture at the same temperature and allow to stir for 30 min.
5. Add (2R)-phenylethylamine (2.0 mL, 16.29 nM, 1.2 equiv) in a dropwise manner at room temperature (RT) and allow the reaction mixture to stir for 12 h.
6. Monitor the progress of reaction using thin layer chromatography by using 9:1 v/v hexane:ethyl acetate (EtOAc; R_f = 0.4) as an eluent.
7. After the reaction is complete, filter the mixture over filter paper (pore size 70 mm).
8. Then, add water (30 mL) into the organic filtrate and extract the organic layer with Et₂O (2 x 50 mL) two times using a separating funnel.
9. Dry the combined organic extracts over 7.5 g of anhydrous Na₂SO₄ and concentrate in vacuo (<15 mbar) using a rotary evaporator.
   NOTE: Now a crude mixture of diastereomeric chiral aziridine containing both isomers of (R)-(1R,2S,5R)-2-isopropyl-5-methylcyclohexyl1-((R)-1-phenylethyl)aziridine-2-carboxylate and (S)-(1R,2S,5R)-2-isopropyl-5-methylcyclohexyl1-((R)-1-phenylethyl)aziridine-2-carboxylate (4.1 g, 90%) is obtained.
10. Isolation of chiral aziridine (R)-(1R,2S,5R)-2-isopropyl-5-methylcyclohexyl 1-((R)-1- phenylethyl)aziridine-2-carboxylate (2) by a selective crystallization method
    1. Add 8.7 g of the crude mixture of chiral aziridine (-)-Menthol ester 1 derivative and dissolve in 70 mL of methanol to an oven-dried 250 mL single-neck round-bottom flask.
    2. Now warm the reaction mixture up to 70 °C by using a hot water bath, then cool the reaction mixture at -10 °C until solid crystal forms.
    3. Filter the solid compound over a filter paper (pore size 70 mm) to obtain 2.2 g of (R)-(1R,2S,5R)-2-isopropyl-5-methylcyclohexyl 1-((R)-1-phenylethyl)aziridine-2-carboxylate (2) ester.
    4. Concentrate the filtrate solution again in vacuo (<15 mbar) using a rotary evaporator, dissolve 50 mL of ethanol in the remaining reaction mixture and recrystallize at -10 °C to obtain 1.2 g of (R)-(1R,2S,5R)-2-isopropyl-5-methylcyclohexyl 1-((R)-1-phenylethyl)aziridine-2-carboxylate (2).
    5. At this time, use the other alcohol ethanol in the same way as methanol.
    6. After recrystallization, again filter over a filter paper (pore size 70 mm), concentrate the remaining crude 5.3 g of filtrate solution completely in vacuo (<15 mbar) using a rotary evaporator, and add 50 mL of a pentane hydrocarbon solvent.
    7. Keep the remaining reaction solution at -15 °C.
       NOTE: Now a solid compound of nearly 1.9 g of (S)-(1R,2S,5R)-2-isopropyl-5-methylcyclohexyl1-((R)-1-phenylethyl)aziridine-2-carboxylate ester (3) is obtained.
    8. After obtaining crystals, concentrate the solution again in vacuo (<15 mbar) using a rotary evaporator and dissolve it in 30 mL of pentane hydrocarbon solvent.
    9. Recrystallize again at -15 °C to obtain 0.8 g of (S)-(1R,2S,5R)-2-isopropyl-5-methylcyclohexyl1-((R)-1-phenylethyl)aziridine-2-carboxylate ester (2').
11. Obtaining (R)-ethyl 1-((R)-1-phenylethyl)aziridine-2-carboxylate (3)
    1. Add (R)-(1R,2S,5R)-2-isopropyl-5-methylcyclohexyl1-((R)-1-phenylethyl)aziridine-2-carboxylate (2) (0.167 g, 0.57 mM) and a magnetic stir-bar into an oven-dried 25 mL two-neck round-bottom flask under nitrogen (N₂) atmosphere.
    2. Add 1.8 mL of ethanol to the reaction flask using an airtight syringe and stir it at RT.
    3. Then add potassium carbonate (0.40 g, 20.28 mmol, 4.0 equiv) and stir it at RT for 2 days.
    4. Monitor the progress of reaction using thin layer chromatography (eluent, 8:2 v/v, hexane:ethyl acetate (EtOAc), R_f = 0.6).
    5. After the reaction is completed, filter the mixture over filter paper (pore size 70 mm), then add water (5 mL) into the organic filtrate and extract the organic layer with CH₂Cl₂ (2 x 15 mL) two times by using a separating funnel.
    6. Dry the combined organic extracts over 3.0 g of anhydrous Na₂SO₄ and concentrate in vacuo (<15 mbar) using a rotary evaporator.
    7. Purify the crude product by normal phase column chromatography on silica gel (70-230 mesh) to afford a pure product (R)-ethyl 1-((R)-1-phenylethyl)aziridine-2-carboxylate (3) (950 mg, 88%). R_f (30% EtOAc/hexane = 0.50).

2. Regio and stereoselective aziridine ring-opening by azide nucleophile for the total synthesis of biemamide B and biemamide D

1. Synthesis of (R)-ethyl 2-azido-3-(((R)-1-phenylethyl)amino)propanoate (5)
   1. Transfer (500 mg, 2.20 mM, 1.0 equiv) of chiral (S)-ethyl 1-((R)-1-phenylethyl)aziridine-2-carboxylate (4) and a magnetic stir-bar into an oven-dried 50 mL two-neck round-bottom flask under open atmosphere.
   2. Add 50% aqueous ethanol (15 mL) to the reaction mixture.
   3. Cool the reaction mixture at 0 °C and add concentrated sulfuric acid (36 N) dropwise to maintain nearly pH 4.0, and stir for 5 min.
   4. Add sodium azide (370 mg, 5.70 mM, 2.5 equiv) at 0 °C and allow the reaction mixture to stir for 10 min at the same temperature and then warm at RT.
   5. Then, add AlCl_3∙6H₂O (55 mg, 0.22 mM, 0.1 equiv) as a catalyst at the same RT and allow to stir for an additional 3 h.
   6. Monitor the progress of reaction using thin layer chromatography (eluent, 6:4 v/v, hexane:ethyl acetate (EtOAc), R_f = 0.2).
   7. Quench the reaction mixture with two portions of 20 mL saturated NaHCO₃.
   8. Then filter the crude mixture over a celite pad with ethanol (2 x 10 mL).
   9. Concentrate the reaction mixture in vacuo (<15 mbar) using a rotary evaporator.
   10. Extract the organic layer with CH₂Cl₂ (2 x 50 mL) two times using a separating funnel.
   11. Then dry the combined organic layer over 5.0 g of anhydrous Na₂SO₄ for 5 min.
   12. Concentrate the crude organic layer in vacuo (<15 mbar) using a rotary evaporator to afford a crude azide product.
   13. Purify the crude product with normal phase column chromatography on silica gel (70-230 mesh) by eluting with 40% EtOAc/hexane (R_f = 0.20) to afford 490 mg (90% yield) of (R)-ethyl 2-azido-3-(((R)-1-phenylethyl)amino)propanoate (5) as a viscous liquid.
2. Synthesis of (9H-Fluoren-9-yl)methyl(3-(((R)-3-methyl-2,4-dioxo-1-((R)-1 phenylethyl)hexahydro pyrimidin-5-yl)amino)-3-oxopropyl)carbamate (7)
   1. Transfer 150 mg of chiral (R)-5-amino-3-methyl-1-((R)-1-phenylethyl) dihydropyrimidine-2,4(1H,3H)-dione (6) (150 mg, 0.60 mM, 1.0 equiv) and a magnetic stir-bar into an oven-dried 25 mL two-neck round-bottom flask under N₂ atmosphere.
   2. Add dry CH₂Cl₂ (15.0 mL) to the reaction flask using an airtight syringe.
   3. Then cool the reaction mixture at 0 °C using an ice bath and stir the reaction mixture for 5 min.
   4. Add Fmoc-beta-alanine (377 mg, 1.20 mM, 2.0 equiv) and DIPEA (0.67 mL, 3.64 mM, 6.0 equiv) at 0 °C and stir it for 5 min.
   5. Add EDCI (347 mg, 1.82 mM, 3.0 equiv) and HOBt (165 mg, 1.21 mM, 2.0 equiv) to the reaction mixture at 0 °C and allow to stir for 10 min at the same temperature.
   6. Keep the reaction mixture at RT and allow to stir for an additional 8 h.
   7. Monitor the progress of reaction using thin layer chromatography using (2:8 v/v, hexane:ethyl acetate (EtOAc), R_f = 0.4) as an eluent.
   8. Quench the reaction mixture with water (10 mL).
   9. Wash the combined organic layer with brine (15 mL), then extract the organic layer with CH₂Cl₂ (2 x 20 mL) two times using a separating funnel.
   10. Then dry the combined organic layer over 5.0 g of anhydrous Na₂SO₄ for 5 min and concentrate it in vacuo (<15 mbar) using a rotary evaporator.
   11. Purify the crude product with normal phase column chromatography on silica gel (70-230 mesh) by eluting with 80% EtOAc/hexane (R_f = 0.40) to afford 295 mg of (7) (90% yield).

3. Stereoselective Mukaiyama aldol reaction with chiral aziridine-2-carboxaldehyde and Its regio and stereoselective aziridine ring-opening by internal hydroxy nucleophile for the total synthesis of (-)- epiallo-somuscarine (17)

1. Synthesis of (S)-4-hydroxy-4-((R)-1-((R)-1-phenylethyl)aziridin-2-yl)butan-2-one (12)
   1. Transfer (R)-1-((R)-1-phenylethyl)aziridine-2-carbaldehyde (10) (140 mg, 0.8 mM, 1.0 equiv) and a magnetic stir-bar into an oven-dried 25 mL two-neck round-bottom flask under N₂ atmosphere.
   2. Add dry CH₃CN (4.0 mL) to the reaction flask using an airtight syringe.
   3. Then cool the reaction mixture at -20 °C using an ice-acetone bath and stir the reaction mixture for 5 min.
   4. Add ZnCl₂ anhydrous (108 mg, 0.8 mM, 1.0 equiv) to the reaction mixture at -20 °C and allow to stir for 5 min.
   5. Then add trimethyl(prop-1-en-2-yloxy)silane (11) (104 mg, 0.8 mM, 1.0 equiv) dissolved in dry CH₃CN (3.0 mL) to the reaction mixture at -20 °C in a dropwise manner and allow the reaction mixture to stir for 1 h at the same temperature.
   6. Monitor the progress of reaction using thin layer chromatography using 8:2 v/v hexane:ethyl acetate (EtOAc), R_f = 0.2) as an eluent.
   7. Quench the reaction mixture with saturated NaHCO₃ (4 mL).
   8. Extract the organic layer with EtOAc (2 x 15 mL) two times using a separating funnel.
   9. Dry the combined organic layer with 3.0 g of anhydrous Na₂SO₄ and concentrate it in vacuo (<15 mbar) using a rotary evaporator.
   10. Purify the crude product with normal phase column chromatography on silica gel (70-230 mesh) by eluting with 80% EtOAc/hexane, (R_f = 0.20) to afford 58 mg of (S)-4-hydroxy-4-((R)-1-((R)-1-phenylethyl)aziridin-2-yl)butan-2-one) (12) (85% yield).
2. Synthesis of (R)-N-(((2R,3S,5R)-3-((tert-butyldimethylsilyl)oxy)-5-methyltetra hydrofuran-2-yl)methyl)-1-phenylethanamine (15)
   1. Transfer (S)-4-((tert-butyldimethylsilyl)oxy)-4-((R)-1-((R)-1-phenylethyl) aziridin-2-yl)butan-2-one (13) (400 mg, 1.15 mM, 1.0 equiv) and a magnetic stir-bar into an oven-dried 25 mL two-neck round-bottom flask under N₂ atmosphere.
   2. Add anhydrous THF (50 mL) to the reaction flask using an airtight syringe.
   3. Cool the reaction mixture at -78 °C using a dry ice-acetone bath and allow to stir for 5 min.
   4. Then add lithium tri-sec-butylborohydride (L-selectride) (1 M solution in THF) (2.3 mL, 2.0 equiv) dropwise into the reaction mixture at -78 °C and allow to stir for another 25 min.
   5. Warm the reaction mixture to RT and allow to stir for 8 h.
   6. Monitor the progress of the reaction by TLC using 30% EtOAc/hexane (R_f = 0.40) as an eluent.
   7. After the complete consumption of compound 13, quench the reaction mixture with 0.1 M of NaOH (5 mL).
   8. Extract the organic layer with EtOAc (3 x 15 mL), then wash with brine (15 mL).
   9. Dry the organic layer over 3.0 g of anhydrous Na₂SO₄ and concentrate in vacuo (<15 mbar) using a rotary evaporator.
   10. Purify the crude product with normal phase column chromatography on silica gel (70-230 mesh, eluent, 8:2 v/v, hexane:ethyl acetate (EtOAc), R_f = 0.2) to afford a pure compound (15) (382 mg, 95% yield).
3. Synthesis of (-)-epiallo-isomuscarine iodide (17)
   1. Transfer compound 16 (20 mg, 0.15 mM, 1.0 equiv) and a magnetic stir bar into an oven-dried 10 mL round-bottom flask under N₂ atmosphere.
   2. Add 3 mL of EtOAc reaction flask using an airtight syringe.
   3. Then add Methyl iodide (0.4 mL, 3.0 mM) to the reaction mixture at RT.
   4. Add 1,2,2,6,6-pentamethylpiperidine (PMP) (0.05 mL, 0.3 mM, 2.0 equiv) to the reaction mixture at 0 °C.
   5. Then, keep the reaction mixture at RT and allow to stir for an additional 16 h.
   6. Evaporate the solvent in vacuo (<15 mbar) using a rotary evaporator to give the crude product.
   7. Wash three times by adding (3 x 5 mL) 10% MeOH in EtOAc to the crude reaction mixture.
   8. Then wash the crude mixture with n-Pentane (5 mL) and concentrate under vacuum (<15 mbar) using a rotary evaporator to achieve pure (-)-epiallo-isomuscarine iodide (17) (32 mg, 68%).

4. Characterization of all products

1. Characterize all the new compounds by ¹H, ¹³C NMR spectroscopy, and high-resolution mass spectrometry (HRMS)^7,8,11.

Wyniki

Here, we report the synthesis of enantiopure aziridine-2-carboxylates. The diastereomeric mixture of (R)-(1R,2S,5R)-2-isopropyl-5-methylcyclohexyl1-((R)-1-phenylethyl)aziridine-2-carboxylate (2) and (S)-(1R,2S,5R)-2-isopropyl-5-methylcyclohexyl1-((R)-1-phenylethyl)aziridine-2-carboxylate (3) (4.1 g, 90%) were prepared in quantitative yield from 2,3 -dibromopropane (-)-mentholyl ester and (1R

Dyskusje

Aziridines as nitrogen-containing three-membered heterocycles have enormous potential for synthetic starting martials or intermediates to prepare nitrogen-rich organic molecules. Based on the group bearing at the ring nitrogen, they are classified as "activated" and "non-activated" aziridines whose chemical reactivity and selectivity are different. However, very limited methods are available to prepare this valuable aziridine in an optically active form.

The protocol in this paper describes a...

Materiały

Name	Company	Catalog Number	Comments
(2R)-1-[(1R)-1-Phenylethyl]-2-aziridinecarboxylic acid (-)-menthol ester, 98%	Sigma-Aldrich	57054-0
(2S)-1-[(1R)-1-Phenylethyl]-2-aziridinecarboxylic acid (-)-menthol ester	Sigma-Aldrich	57051-6
1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride	TCI	424331-25 g	CAS No: 25952-53-8
1,4-Dioxane	SAMCHUN	D0654-1 kg	CAS No: 123-91-1
1-Hydroxybenzotriazole hydrate	Aldrich	219-989-7-50 g	CAS No: 123333-53-9
2,6-Lutidine	Alfa Aesar	A10478-AP, 500 mL	CAS No: 108-48-5
Acetonitrile	SAMCHUN	A0127-18  L	CAS No: 75-05-8
Acetonitrile-d3	Cambridge Isotope Laboratories,	15G-744-25 g	CAS No: 2206-26-0
Aluminum chloride hexahydrate	Aldrich	231-208-1, 500 g	CAS No : 7784-13-6
Bruker AVANCE III HD (400 MHz) spectrometer	Bruker	NA
Chloroform-d	Cambridge Isotope Laboratories,	100 g	CAS No: 865-49-6
Dichloromethane	SAMCHUN	M0822-18 L	CAS No: 75-09-2
Dimethyl sulfoxide-d6	Cambridge Isotope Laboratories,	25 g	CAS No: 2206-27-1
Ethanol	EMSURE	1009831000,1L	CAS No: 64-17-5
Ethyl acetate	SAMCHUN	E0191-18 L	CAS No: 141-78-6
High resolution mass spectra/MALDI-TOF/TOF Mass Spectrometry	AB SCIEX	4800 Plus	High resolution mass spectra
JASCO P-2000	JASCO	P-2000	For optical rotation
Lithium aluminum hydride	TCI	L0203-100 g	CAS No: 16853-85-3
L-Selectride, 1 M solution in THF	Acros	176451000, 100 mL	CAS No: 38721-52-7
Methanol	SAMCHUN	M0585-18 L	CAS No: 67-56-1
N-[(9H-Fluoren-9-ylmethoxy)carbonyl]-β-alanine	TCI	F08825G-5 g	CAS No: 35737-10-1
N-Ethyldiisopropylamine	Aldrich	230-392-0, 100 mL	CAS No: 7087-68-5
n-Hexane	SAMCHUN	H0114-18 L	CAS No: 110-54-3
Ninhydrin	Alfa Aesar	A10409-250 g	CAS No: 485-47-2
p-Anisaldehyde	aldrich	A88107-5 g	CAS No: 123-11-5
Phosphomolybdic acid hydrate	TCI	P1910-100 g	CAS No: 51429-74-4
Sodium azide	D.S.P	703301-500 g	CAS No: 26628-22-8
Sodium Hydride 60% dispersion in mineral oil	Sigma-Aldrich	452912-100 G	CAS No: 7646-69-7
Sodium hydroxide	DUKSAN	A31226-1 kg	CAS No: 1310-73-2
Sodium sulfate	SAMCHUN	S1011-1 kg	CAS No: 7757-82-6
Thin Layer Chromatography (TLC)	Merck	100390
Tert-Butyldimethylsilyl trifluoromethanesulfonate, 98%	Aldrich	274-102-0, 25 g	CAS NO: 69739-34-0
Tetrahydrofuran	SAMCHUN	T0148-18 L	CAS No: 109-99-9
Triethylethylamine	DAEJUNG	8556-4400-1 L	CAS No: 121-44-8
UV light	Korea Ace Sci	TN-4C	254 nm
Zinc chloride, anhydrous, 98+%	Alfa Aesar	A16281-22100 g	CAS No : 7646-85-7