Standardized Rat Coronary Ring Preparation and Real-Time Recording of Dynamic Tension Changes Along Vessel Diameter

Podsumowanie

The present protocol describes the wire myograph technique for measuring vascular reactivity of the rat coronary artery.

Streszczenie

As a key event of cardiovascular system diseases, coronary artery disease (CAD) has been widely regarded as the main culprit of atherosclerosis, myocardial infarction, and angina pectoris, which seriously threaten the life and health of people all over the world. However, how to record the dynamic biomechanical characteristics of isolated blood vessels has long puzzled people. Meanwhile, precise positioning and isolation of coronary arteries to measure in vitro dynamic vascular tension changes have become a trend in CAD drug development. The present protocol describes the macroscopic identification and microscopic separation of rat coronary arteries. The contraction and dilation function of the coronary artery ring along the vessel diameter was monitored using the established multi myograph system. The standardized and programmed protocols of coronary ring tension measurement, from sampling to data acquisition, tremendously improve the repeatability of the experimental data, which ensures the authenticity of vascular tension records after physiological, pathological, and drug intervention.

Wprowadzenie

Coronary artery disease (CAD) has been widely recognized and concerned as a typical and representative cardiovascular disease, being the leading cause of death in both developed and developing countries^1,2. As a blood and oxygen supply route for normal cardiac physiological function, circulating blood enters and nourishes the heart through two main coronary arteries and a blood vascular network on the surface of the myocardium^3,4. Cholesterol and fat deposits in the coronary arteries cut off the heart's blood supply and the violent inflammatory res....

Protokół

The animal protocol was reviewed and approved by the Management Committee from Chengdu University of Traditional Chinese Medicine (Record No. 2021-11). Male Sprague Dawley (SD) rats (260-300 g, 8-10 weeks old) were used for the present study. The rats were kept in an animal chamber and were free to drink and eat during the experiment.

1. Solution preparation

1. Prepare physiological salt solution (PSS) by dissolving 118 mM of NaCl, 4.7 mM of K⁺, 2.5 mM of CaCl₂, 1.2 mM of KH₂PO₄, 1.2 mM of MgCl₂∙6H₂O, 25 mM of NaHCO₃, 11 mM of D-gluc....

Wyniki

Anatomically positioned, rat coronary arteries distributed and hidden deep in myocardial tissue were not easily recognized. By comparing the coronary arteries of humans (Figure 1A) and rats (Figure 1B), rapid and accurate separation of rat coronary arteries was conducted according to the sampling process in Figure 2. After precisely locating the right auricle, pulmonary artery, and apex from the front under an optical microscope, th.......

Dyskusje

The disturbance of coronary microcirculation, which involves a wide range of patients with CAD, has been gradually recognized and concerned the basis for adequate myocardium perfusion. Considering the serious complications of sudden coronary heart disease and cardiovascular disease, timely drug prevention and treatment are extremely important for a clinical individual with CAD¹⁷. Inevitably, the secrecy of coronary artery anatomy and the complexity of its physiological structure have severely rest.......

Materiały

Name	Company	Catalog Number	Comments
Apigenin	Sangon Biotech Co., Ltd., Shanghai, China	150731
CaCl2	Sangon Biotech Co., Ltd., Shanghai, China	A501330
D-glucose	Sangon Biotech Co., Ltd., Shanghai, China	A610219
HEPES	Xiya Reagent Co., Ltd., Shandong, China	S3872
KCl	Sangon Biotech Co., Ltd., Shanghai, China	A100395
KH2PO4	Sangon Biotech Co., Ltd., Shanghai, China	A100781
LabChart Professional version 8.3	ADInstruments, Australia	—
MgCl2·6H2O	Sangon Biotech Co., Ltd., Shanghai, China	A100288
Multi myograph system	Danish Myo Technology, Aarhus, Denmark	620M
NaCl	Sangon Biotech Co., Ltd., Shanghai, China	A100241
NaHCO3	Sangon Biotech Co., Ltd., Shanghai, China	A100865
Steel wires	Danish Myo Technology, Aarhus, Denmark	400447
U46619	Sigma, USA	D8174