A Streamlined and Standardized Procedure for Generating High-Titer, High-Quality Adeno-Associated Virus Vectors Utilizing a Cell Factory Platform

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1.2K Views

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05:51 min

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May 3rd, 2024

DOI :

10.3791/66741-v

May 3rd, 2024

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Ting Zhang¹, Vinitha Dhamotharan¹, Yu Xiao¹^,², Sydne Ballengee¹^,³, Jill Pollon¹^,⁴, George K. Gittes¹

¹Division of Pediatric Surgery, Department of Surgery, Children's Hospital of Pittsburgh, University of Pittsburgh School of Medicine, ²North Allegheny High School, ³Wright State University Boonshoft School of Medicine, ⁴College of Engineering, University of Michigan

Podsumowanie

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Adeno Associated Virus AAV

High titer Production

Cell Factory Platform

Purification Process

Scalable Culture Conditions

Two phase Partitioning

Yield And Purity

HEK293T Cells

Gene Therapy Development

Preclinical Studies

SDS PAGE

Transmission Electron Microscopy TEM

Cell Stacks CF10

Gradient Ultracentrifugation

Rozdziały w tym wideo

0:00

Introduction

1:50

Transfection and Harvesting Adeno‐Associated Virus Vectors Using HEK293T Cells for Gene Therapy

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