Name: transurethral instillation procedure in adult male mouse
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Description: Watch this Scientific Journal Video about Transurethral Instillation Procedure in Adult Male Mouse at JoVE.com

The authors do not have any acknowledgements.

All the procedures with animals, including the method and duration of anesthesia, as well as postoperative care, were discussed with a veterinarian and approved by the Institutional Animal Care and Use Committee (IACUC) at the affiliated institution. In this manuscript, all procedures were performed with 8 to 15-week-old C57BL/6 male mice and the protocol follows the animal care guidelines approved by the IACUC at the University of Colorado.
1. Preparation of Tubing
NOTE: To instill the inoculum, a needle attached to the polyethylene (PE) tubing (Figure 1A) or a small gauge (&#60;24 G) angiocatheter can be used (Figure 1G). The use of an angiocatheter requires no preparation other than sterilization with 70% ethanol (EtOH). The inoculum is the substance that is instilled/injected/deposited into the urinary tract. This can be a solution containing bacteria, virus, or a chemical. This is prepared ahead of time and per the research protocol. For example, if the study aims to evaluate the effects of E. coli infection on the lower urinary tract, the researcher will need to prepare the inoculum containing bacteria, which can be inoculated/instilled in the lower urinary tract.
<ol>
	<li>Preparation of the needle attached to PE tubing is simple and consists of the following: Choose the size of PE tubing based on the target organ and the age of an animal. For an animal younger than 12 weeks, use PE-10 tubing (outside diameter is 0.024 inches and inside diameter is 0.011 inches). PE-10 tubing is suggested to be applicable for (1) the primary seeding of inoculum in the anterior prostate (AP) and seminal vesicles (SV), or (2) the primary seeding of inoculum in the bladder of an animal younger than 12 weeks of age. PE tubing with a larger diameter of up to size PE 50 (outside diameter up to 0.038 inches and inside diameter up to 0.023 inches) can be used for the primary seeding of inoculum in the bladder of the older animals.</li>
	<li>Cut approximately 2.5 inches of PE tubing.</li>
	<li>Select a needle that will fit the selected PE diameter to be used. A 27 G needle can fit into the lumen of PE-10 tubing, and a 22 G needle fits the lumen of PE-50 tubing.
	<ol>
		<li>Prepare the needle prior to insertion into the PE tubing by rubbing the sharp edge of the needle on a commercially available oil stone (Figure 1A-B) to make it dull, or use a commercially available blunt needle.</li>
	</ol>
	</li>
	<li>Introduce the dull needle (Figure 1C) into the lumen of the PE tubing. Avoid damage to the lumen or entry into a false lumen by ensuring the needle remains in the center of lumen. (Figure 1D-E). If the PE tubing is damaged during insertion of the dull needle, cut the damaged end and restart the process.</li>
	<li>Place the needle-attached PE tubing in 70% of EtOH for sterilization until its use for the procedure. Flush the 70% EtOH through the needle-PE tubing to sterilize the lumen and to ensure there is no leakage from the PE tubing from damage during the process of needle insertion. If there is leakage, discarded the needle-PE tubing. 
	NOTE: The tip of the PE tubing needs to be smooth prior to catheterization. To avoid issues with introducing the PE tubing into the urethra, it is recommended to cut the tip of the PE tubing immediately prior to catheterization (Figure 1F). The suggested minimum length of the PE tubing is 1.5 inches, which includes the part surrounding the needle.</li>
</ol>
2. Catheterization Procedure
<ol>
	<li>Prior to induction of anesthesia, empty the bladder of mouse.
	<ol>
		<li>Apply gentle pressure and massage to the lower abdomen of the mouse. These maneuvers typically lead to spontaneous voiding. A full bladder may result in the dilution or immediate leakage any instilled inoculum. 
		NOTE: An additional option to ensure an empty bladder in a mouse is to deprive the animal of water for at least 1 hour before the start of the transurethral instillation procedure. Water deprivation should be approved by the institution as part of the animal protocol.</li>
	</ol>
	</li>
	<li>Use an anesthesia machine with isoflurane flow (2%) to induce anesthesia.
	<ol>
		<li>Once the animal is anesthetized, place it on a warm heating blanket and cover it with a blue pad with its nose inside a nosecone for the maintenance of continuous anesthesia.</li>
		<li>Confirm that the animal is under anesthesia by checking the pedal reflex (toe pinch).</li>
	</ol>
	</li>
	<li>Once the pedal reflex is absent, proceed by retracting the prepuce.
	<ol>
		<li>Press the pubic region with both the thumb and index finger of one hand (Figures 2A and 2B), and pull out the glands penis using the thumb and index finger of the other hand (Figure 2C). When this is accomplished correctly, the prepuce, glans penis, and penile body remain exposed. (Figure 2D and Figure 2F). Do not use forceps to pull out glands penis as this will result in damage to the tissue.</li>
	</ol>
	</li>
	<li>Lubricate the tip of the catheter and the glans with sterile surgical lubricant prior to insertion of catheter. This will facilitate insertion of the PE tubing. 
	NOTE: Individually packaged squares of surgical lubricant, instead of one large tube, are recommended for each use to maintain sterile conditions.</li>
	<li>Gently hold the tip of the penis using dull forceps and gently squeeze the glans to cause the urethral meatus to open.</li>
	<li>Hold the syringe with the needle-attached PE-tubing or PE tubing itself and insert the PE tubing through the urethral meatus. 
	NOTE: A tripod grip of the syringe with the needle-attached PE-tubing or the PE tubing itself using the thumb, index, and middle fingers is recommended for stability. 
	NOTE: The path of catheterization should be curved due to the natural curvature of the urethra surrounded by the glans penis and penile body in the adult male mouse as shown in Figure 3A and Figure 3B. The catheterized PE tubing may meet resistance during catheterization, therefore, it is recommended to adjust the angle by tilting the tubing up or down to help advance the PE tubing further to the target organ. If needed, the PE tubing can be slightly rotated to help it maneuver through the various sharp turns of urethra. When there is too much resistance, the tubing should be pulled back and then advance again with minimal pressure. Pulling the PE-tubing out completely and using more lubrication may be helpful.</li>
	<li>Perform instillation of the inoculum once the PE-tubing has reached the desired organ by pushing the needle plunger to instill the desired volume of the inoculum (5-200 &#181;L volume is suggested).
	<ol>
		<li>For instillation of the inoculum into the urinary bladder, advance the PE tubing until approximately 0.75 inches of PE tubing is inserted (Figure 3A). 
		NOTE: For instillation of the inoculum in the AP and SV, generally, the inserted tubing meets resistance once it reaches the point of opening into the anterior prostate. By adjusting the angle by tilting the tubing up or down, the PE tubing will advance further until approximately 1 inch of PE tubing has been inserted (Figure 3B).</li>
	</ol>
	</li>
</ol>
3. In Vivo Imaging of the Lower Urinary Tract and Kidneys
NOTE: The purpose of in vivo imaging in this manuscript is: (1) to confirm the spatial localization of the inoculum; and (2) to assess the incidence of reflux of the inoculum to the kidneys. For this purpose, either an anesthetized or euthanized mouse can be used.
<ol>
	<li>To visualize the lower urinary tract of an adult male mouse, perform lower abdominal midline laparotomy to expose the bladder, prostate, and seminal vesicles.
	<ol>
		<li>Incise the skin in the midline from the pubic bone to just below the xiphoid process using scissors.</li>
		<li>Elevate the skin off the subcutaneous tissue.</li>
		<li>Identify the avascular linea alba located in the midline, and incise this to enter the peritoneal cavity where all the organs are located.</li>
		<li>Confirm the spatial localization by visualization of the blue dye or another suitable tracking agent.</li>
	</ol>
	</li>
</ol>

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The authors have nothing to disclose.

This manuscript describes in detail a method of transurethral instillation in adult male mouse. Differential primary seeding area can be reached by using PE tubing of different diameters. PE-50 is recommended for successfully reaching the urinary bladder lumen, while a larger diameter PE-10 is used to reach the AP and SV (Figure 3A-B). In addition to the choice of size of PE tubing and target organ for inoculation, other factors, such as the volume of instilled inoculum, als...

The transurethral instillation approach has been used in previous studies as one of the methods to create rodent models of bladder pathologies1,2,3,4 and can be used to evaluate the effectiveness of locally delivered treatments in mice. Although animal models cannot fully recapitulate human pathologies, identification of the underlying mechanisms in animal studies provides a better understanding of the human LUT disorders such as interstitial cystitis/bladder pain syndrome, neurogenic cystitis, autoimmune cystitis, and prostatic inflammation5.
The transurethral instillation procedure performed on an adult male mouse is more technically challenging than intravesical instillation in an adult female mouse6. The naturally curved anatomy of the male urethra along with its small diameter make it technically difficult to accomplish the transurethral insertion of a catheter. Therefore, detailed instructions for the transurethral induction of mouse urinary tract infection6 and LUT inflammation7,8,9,10,11,12,13,14,15 via bladder-inserted catheter were previously outlined for female mice only. This manuscript aims to provide a step-by-step description of the technique for transurethral instillation of substances in male mice including the video clips, images, and illustrations. Transurethral instillation procedures in an adult male mouse can be performed with variable inoculates similar to that previously described in the female mouse7 and merged with additional techniques such as electromyogram recordings of visceromotor responses (VMR)16.

<table><tbody><tr><td>BD INTRAMEDIC Polyethylene Tubing/ PE 10</td><td>BD Medical</td><td>63019-004</td><td></td></tr><tr><td>BD INTRAMEDIC Polyethylene Tubing/ PE 50</td><td>BD Medical</td><td>63019-048</td><td></td></tr><tr><td>BD Insyte-N Autoguard/ Angiocath</td><td>BD Medical</td><td>311411</td><td></td></tr><tr><td>Ethanol (EtOH)</td><td>Fisher Scientific</td><td>BP2818500</td><td></td></tr><tr><td>BD Needle Only 22 Gauge (G) 1 inch</td><td>Becton Dickson</td><td>205155</td><td></td></tr><tr><td>BD Needle Only 27 Gauge (G) 1/2 inch</td><td>Becton Dickson</td><td>305129</td><td></td></tr><tr><td>Luer stub, 22 G X 0.5 in / Blunt Needle</td><td>Instech</td><td>LS22</td><td></td></tr><tr><td>Luer stub, 27 G X 0.5 in / Blunt Needle</td><td>Instech</td><td>LS27</td><td></td></tr><tr><td>6&quot; Long x 2&quot; Wide x 1&quot; Thick, Aluminum Oxide Sharpening Stone / Oil Stone</td><td>Norton</td><td>40204034</td><td></td></tr><tr><td>Surgical Lubricant Sterile Bacteriostatic Folilpac</td><td>Surgilube</td><td>281020543</td><td></td></tr><tr><td>Isoflurane</td><td>Fluriso</td><td>13985-528-60</td><td></td></tr><tr><td>SomnoSuite Low-Flow Anesthesia System</td><td>Kent Scientific</td><td>SS-01</td><td></td></tr><tr><td>Dissecting Forceps, Curved</td><td>vwr</td><td>82027-392</td><td></td></tr><tr><td>BD 1 ml Syringe</td><td>BD Medical</td><td>309626</td><td></td></tr><tr><td>India Ink Solution., 0.2% in PBS buffer</td><td>Alfa Aesar</td><td>J61007</td><td></td></tr><tr><td>8~12 weeks old male C57B/6 Mice</td><td>Jackson Lab</td><td>#000664</td><td></td></tr></tbody></table>

transurethral instillation procedure in adult male mouse

Transurethral instillation can be used to deliver different solutions with active ingredients (e.g., drugs, chemicals, bacteria, and viruses) locally into the urinary bladder to either induce animal models of bladder pathologies or evaluate the effectiveness of intravesical treatments. Most rodent models of lower urinary tract (LUT) pathologies are induced in female mice due to ease of intravesical instillation of the substances via the female urethra. However, due to anatomical differences between the female and male LUT, transurethral instillation in a male mouse has been deemed a very challenging procedure, and it has not been previously described. In this manuscript, we provide a detailed description of how to prepare polyethylene (PE) tubing for subsequent insertion into the urethra of a male mouse. In addition, we discuss the ideal types of PE tubing to be used depending on the desired site of inoculation. Furthermore, we describe point by point how to prepare an animal for a successful transurethral instillation to avoid injury to the urethra and ensure the delivery of the solution to the desired location. The procedure is started by retracting the prepuce and the glans to expose the opening of the urethral meatus. Next, the glans are grasped by blunt non-crushing forceps to stabilize the penis and the PE tubing. The PE tubing is first inserted into the urethral meatus parallel to the animal body, then its angle is adjusted by tilting the catheter to maneuver it to follow the natural curvature of the urethra. This technique can be used to induced murine models of bladder pathologies and/or evaluate the effectiveness of intravesical treatments in male mice.

Transurethral instillation of blue dye via PE 50 tubing resulted in instillation of the dye into the urinary bladder (Figure 4A), and via PE 10 tubing in colorization of the SV and AP (Figure 4B), respectively. To evaluate whether the performed transurethral instillation causes an instant reflux of the inoculum to the kidneys or not, the kidney and ureter were observed followed by instillation of 100 &#181;L of the blue dye and f...

Watch this Scientific Journal Video about Transurethral Instillation Procedure in Adult Male Mouse at JoVE.com

Transurethral Instillation Procedure in Adult Male Mouse

Transurethral instillation is a challenging procedure and has not been well described in the literature. The aim of this manuscript is to describe a technique for transurethral insertion of a catheter for intravesical delivery of liquids with active substances into the urinary bladder and/or prostate in adult male mice.

Transurethral instillation can be used to deliver different solutions with active ingredients (e.g., drugs, chemicals, bacteria, and viruses) locally into ...

transurethral-instillation-procedure-in-adult-male-mouse

Research

JoVE Journal

Immunology and Infection

15.5K Views.  University of Colorado School of Medicine. Transurethral instillation is a challenging procedure and has not been well described in the literature. The aim of this manuscript is to describe a technique for transurethral insertion of a catheter for intravesical delivery of liquids with active substances into the urinary bladder and/or prostate in adult male mice.

Video: Transurethral Instillation Procedure in Adult Male Mouse

Transurethral Induction of Mouse Urinary Tract Infection

Uropathogenic bacterial strains of interest are grown on agar. Generally, uropathogenic E. coli (UPEC) and other strains can be grown overnight on Luria-Bertani (LB) agar at 37&deg;C in ambient air. UPEC strains grow as yellowish-white translucent colonies on LB agar. Following confirmation of appropriate colony morphology, single colonies are then picked to be cultured in broth. LB broth can be used for most uropathogenic bacterial strains. Two serial, overnight LB broth cultures can be employed to enhance expression of type I pili, a well-defined virulence factor for uropathogenic bacteria. Broth cultures are diluted to the desired concentration in phosphate buffered saline (PBS). Eight to 12 week old female mice are placed under isoflurane anesthesia and transurethrally inoculated with bacteria using polyethylene tubing-covered 30 gauge syringes. Typical inocula, which must be empirically determined for each bacterial/mouse strain combination, are 106 to 108 cfu per mouse in 10 to 50 microliters of PBS. After the desired infection period (one day to several weeks), urine samples and the bladder and both kidneys are harvested. Each organ is minced, placed in PBS, and homogenized in a Blue Bullet homogenizer. Urine and tissue homogenates are serially diluted in PBS and cultured on appropriate agar. The following day, colony forming units are counted.

This video will demonstrate methods to transurethrally induce mouse urinary tract infections and quantify the extent of resulting infections.

Uropathogenic bacterial strains of interest are grown on agar. Generally, uropathogenic E. coli (UPEC) and other strains can be grown overnight on ...

Mouse Bladder Wall Injection

Mouse bladder wall injection is a useful technique to orthotopically study bladder phenomena, including stem cell, smooth muscle, and cancer biology. Before starting injections, the surgical area must be cleaned with soap and water and antiseptic solution. Surgical equipment must be sterilized before use and between each animal. Each mouse is placed under inhaled isoflurane anesthesia (2-5% for induction, 1-3% for maintenance) and its bladder exposed by making a midline abdominal incision with scissors. If the bladder is full, it is partially decompressed by gentle squeezing between two fingers. The cell suspension of interest is intramurally injected into the wall of the bladder dome using a 29 or 30 gauge needle and 1 cc or smaller syringe. The wound is then closed using wound clips and the mouse allowed to recover on a warming pad. Bladder wall injection is a delicate microsurgical technique that can be mastered with practice.

Mouse bladder wall injection is a useful approach to orthotopically study bladder stem cell and cancer biology. This delicate microsurgical method can be mastered with careful technique and practice.

Mouse bladder wall injection is a useful technique to orthotopically study bladder phenomena, including stem cell, smooth muscle, and cancer biology. ...

Medicine

Urinary Tract Infection in a Small Animal Model: Transurethral Catheterization of Male and Female Mice

Urinary tract infections (UTI) are extremely common worldwide, incurring significant morbidity and healthcare-associated expenses. Small animal models, which accurately reflect disease establishment and progression, permit dissection of host-pathogen interactions and generation of immunity to infection. In mice, intravesical instillation of uropathogenic E. coli, the causative agent in more than 85% of community acquired UTI, recapitulates many of the stages of infection observed in humans. Until recently, however, UTI could only be modeled in female animals. This limitation has hindered the study of sex-related differences in UTI, as well as other bladder pathologies, such as cancer. Here, we describe a method to instill male mice that allows direct comparison between female and male animals and provide a detailed protocol to assess bladder tissue by flow cytometry as a means to better understand host responses to infection. Together, these approaches will aid in the identification of host factors that contribute to sex biases observed in UTI and other bladder-associated diseases.

The established model of transurethral catheterization of mice allows the study of bladder pathologies, including urinary tract infection, but can only be performed in females. A new model of male transurethral instillation, presented here, will enable research in an area marked by strong clinical and epidemiological differences between the sexes.

Urinary tract infections (UTI) are extremely common worldwide, incurring significant morbidity and healthcare-associated expenses. Small animal models, ...

Longitudinal Follow-Up of Urinary Tract Infections and Their Treatment in Mice using Bioluminescence Imaging

Urinary tract infections (UTI) rank among the most common bacterial infections in humans and are routinely treated with empirical antibiotics. However, due to increasing microbial resistance, the efficacy of the most used antibiotics has declined. To find alternative treatment options, there is a great need for a better understanding of the UTI pathogenesis and the mechanisms that determine UTI susceptibility. In order to investigate this in an animal model, a reproducible, non-invasive assay to study the course of UTI is indispensable.
For years, the gold standard for the enumeration of bacterial load has been the determination of Colony Forming Units (CFU) for a particular sample volume. This technique requires post-mortem organ homogenates and serial dilutions, limiting data output and reproducibility. As an alternative, bioluminescence imaging (BLI) is gaining popularity to determine the bacterial load. Labeling pathogens with a lux operon allow for the sensitive detection and quantification in a non-invasive manner, thereby enabling longitudinal follow-up. So far, the adoption of BLI in UTI research remains limited.
This manuscript describes the practical implementation of BLI in a mouse urinary tract infection model. Here, a step-by-step guide for culturing bacteria, intravesical instillation and imaging is provided. The in vivo correlation with CFU is examined and a proof-of-concept is provided by comparing the bacterial load of untreated infected animals with antibiotic-treated animals. Furthermore, the advantages, limitations, and considerations specific to the implementation of BLI in an in vivo UTI model are discussed. The implementation of BLI in the UTI research field will greatly facilitate research on the pathogenesis of UTI and the discovery of new ways to prevent and treat UTI.

This manuscript describes the intravesical administration of uropathogenic bacteria with a lux operon to induce a urinary tract infection in mice and subsequent longitudinal in vivo analysis of the bacterial load using bioluminescence imaging.

Urinary tract infections (UTI) rank among the most common bacterial infections in humans and are routinely treated with empirical antibiotics. However, ...

Ultrasonography of the Adult Male Urinary Tract for Urinary Functional Testing

The incidence of clinical benign prostatic hyperplasia (BPH) and lower urinary tract symptoms (LUTS) is increasing due to the aging population, resulting in a significant economic and quality of life burden. Transgenic and other mouse models have been developed to recreate various aspects of this multifactorial disease; however, methods to accurately quantitate urinary dysfunction and the effectiveness of new therapeutic options are lacking. Here, we describe a method that can be used to measure bladder volume and detrusor wall thickness, urinary velocity, void volume and void duration, and urethral diameter. This would allow for the evaluation of disease progression and treatment efficacy over time. Mice were anesthetized with isoflurane, and the bladder was visualized by ultrasound. For non-contrast imaging, a 3D image was taken of the bladder to calculate volume and evaluate shape; the bladder wall thickness was measured from this image. For contrast-enhanced imaging, a catheter was placed through the dome of the bladder using a 27-gauge needle connected to a syringe by PE50 tubing. A bolus of 0.5 mL of contrast was infused into the bladder until a urination event occurred. Urethral diameter was determined at the point of the Doppler velocity sample window during the first voiding event. Velocity was measured for each subsequent event yielding a flow rate. In conclusion, high frequency ultrasound proved to be an effective method for assessing bladder and urethral measurements during urinary function in mice. This technique may be useful in the assessment of novel therapies for BPH/LUTS in an experimental setting.

We describe the use of high frequency ultrasound with contrast imaging as a method to measure bladder volume, bladder wall thickness, urine velocity, void volume, void duration, and urethral diameter. This strategy can be used to assess voiding dysfunction and treatment efficacy in various mouse models of lower urinary tract dysfunction (LUTD).

The incidence of clinical benign prostatic hyperplasia (BPH) and lower urinary tract symptoms (LUTS) is increasing due to the aging population, resulting ...

Novel Method of Plasmid DNA Delivery to Mouse Bladder Urothelium by Electroporation

Genetically engineered mouse models (GEMMs) are extremely valuable in revealing novel biological insights into the initiation and progression mechanisms of human diseases such as cancer. Transgenic and conditional knockout mice have been frequently used for gene overexpression or ablation in specific tissues or cell types in vivo. However, generating germline mouse models can be time-consuming and costly. Recent advancements in gene editing technologies and the feasibility of delivering DNA plasmids by viral infection have enabled rapid generation of non-germline autochthonous mouse cancer models for several organs. The bladder is an organ that has been difficult for viral vectors to access, due to the presence of a glycosaminoglycan layer covering the urothelium. Here, we describe a novel method developed in lab for efficient delivery of DNA plasmids into the mouse bladder urothelium in vivo. Through intravesical instillation of pCAG-GFP DNA plasmid and electroporation of surgically exposed bladder, we show that the DNA plasmid can be delivered specifically into the bladder urothelial cells for transient expression. Our method provides a fast and convenient way for overexpression and knockdown of genes in the mouse bladder, and can be applied to building GEMMs of bladder cancer and other urological diseases.

We describe a novel method for the delivery of DNA plasmid into the urothelial cells of mouse bladder in vivo through urethra catheterization and electroporation. It offers a fast and convenient way for generating autochthonous mouse models of bladder diseases.

Genetically engineered mouse models (GEMMs) are extremely valuable in revealing novel biological insights into the initiation and progression mechanisms ...

Biology

Modeling Orthotopic Bladder Tumor in Mouse Model: A Procedure for Intravesical Administration of Cancer Cells into Murine Bladder

Source: Tham, S. M. et al. <a href="https://www.jove.com/video/55078" target="_blank">A Murine Orthotopic Bladder Tumor Model and Tumor Detection System.</a>&#160;J. Vis. Exp.&#160;(2017)
In this video, we describe the intravesical procedure of delivering cancer cells into the bladder of a mouse model. This procedure helps generate an orthotopic bladder tumor model that can be used to develop therapeutic strategies.

Modelowanie ortotopowego guza pęcherza moczowego w modelu mysim: procedura dopęcherzowego podawania komórek rakowych do mysiego pęcherza moczowego

Source: Tham, S. M. et al. A Murine Orthotopic Bladder Tumor Model and Tumor Detection System.&#160;J. Vis. Exp.&#160;(2017)
In this video, we describe ...

JoVE Encyclopedia of Experiments

Cancer Research

Urinary Tract Cancer

In vivo studies

Urine Depletion and Bladder Rinsing in Mouse Model: A Catheter-based Procedure to Empty and Wash Murine Urinary Bladder

Source: Yu, C. et al. <a href="https://www.jove.com/video/57649" target="_blank">Novel Method of Plasmid DNA Delivery to Mouse Bladder Urothelium by Electroporation.</a> J. Vis. Exp. (2018)
In this video, we describe a catheter-based procedure to empty and rinse the urinary bladder of a mouse model. The prepped mouse can then be used for further experimental procedures.

Wyczerpanie moczu i płukanie pęcherza moczowego w modelu mysim: procedura oparta na cewniku do opróżniania i mycia mysiego pęcherza moczowego

Source: Yu, C. et al. Novel Method of Plasmid DNA Delivery to Mouse Bladder Urothelium by Electroporation. J. Vis. Exp. (2018)
In this video, we describe ...

Transurethral Catheterization and Bacterial Inoculation to Generate a Urinary Tract Infection Model in Male Mice

Source: Zychlinsky Scharff, A., et al. <a href="https://app.jove.com/v/54432">Urinary Tract Infection in a Small Animal Model: Transurethral Catheterization of Male and Female Mice.</a> J. Vis. Exp. (2017).
This video demonstrates the establishment of uropathogenic Escherichia coli infection in a female mouse bladder through transurethral catheter instillation. The injected bacteria successfully establish a urinary tract infection within the bladder, providing a valuable platform for studying healthcare-associated diseases related to the urinary tract.

Cewnikowanie przezcewkowe i inokulacja bakteryjna w celu wygenerowania modelu infekcji dróg moczowych u samców myszy

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board.
1. ...

Immunology

Immunopathology

Host-Pathogen Interaction Models

Establishment of a Urinary Tract Infection Model in Female Mice

Source: Zychlinsky Scharff, A. et al., <a href="https://app.jove.com/v/54432">Urinary Tract Infection in a Small Animal Model: Transurethral Catheterization of Male and Female Mice.</a>&#160;J. Vis. Exp. (2017)
This video demonstrates the establishment of uropathogenic Escherichia coli infection in a female mouse bladder through transurethral catheter instillation. The injected bacteria successfully establish a urinary tract infection within the bladder, providing a valuable platform for studying healthcare-associated diseases related to the urinary tract.