A Luciferase-fluorescent Reporter Influenza Virus for Live Imaging and Quantification of Viral Infection

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25.0K Views

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05:21 min

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August 14th, 2019

DOI :

10.3791/59890-v

August 14th, 2019

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Kevin Chiem¹, Javier Rangel-Moreno², Aitor Nogales¹^,³, Luis Martinez-Sobrido¹

¹Department of Microbiology and Immunology, University of Rochester School of Medicine and Dentistry, ²Division of Allergy/Immunology and Rheumatology, Department of Medicine, University of Rochester, ³Center for Animal Health Research, INIA-CISA

Podsumowanie

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Intranasal Inoculation

Nluc Luciferase Substrate

Mice Handling

Ex Vivo Imaging

Region Of Interest ROI

Imaging Parameters

Tissue Acquisition

Rozdziały w tym wideo

0:04

Title

1:04

Mouse Infection

1:32

Bioluminescence Monitoring of Mice Infected with BIRFLU

2:32

Fluorescence Analysis in Mice Infected with BIRFLU

3:32

Results: BIRFLU Characterization and Tracking

4:58

Conclusion

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