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Presynapse Formation Assay Using Presynapse Organizer Beads and “Neuron Ball” Culture

8.1K Views

10:17 min

August 2nd, 2019

DOI :

10.3791/59893-v

August 2nd, 2019


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Presynapse Formation

Rozdziały w tym wideo

0:04

Title

0:55

Preparation of Neuron Balls as Hanging Drop Culture

3:24

Placing Neuron Balls on PLL-Coated Glass Coverslips and Culture Maintenance

4:29

Applying LRRTM2 Beads on Neuron Ball Culture With or Without Cell Bodies

6:07

Fluorescence Microscopy

7:19

Results: Analysis of Accumulation of Presynaptic Proteins in LRRTM2-Induced Presynapses of Axons of Neuron Ball Culture

9:15

Conclusion

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